Influences and mechanisms of nanofullerene on the horizontal transfer of plasmid-encoded antibiotic resistance genes between E. coli strains

Highlights

• Sub-inhibitory levels of nC₆₀ promote conjugative transfer of ARGs.

• nC₆₀ can induce ROS generation, oxidative stress and SOS response.

• nC₆₀ can increase cell membrane permeability and alter gene expression.

• Results provide evidence of nC₆₀ promoting antibiotic resistance dissemination.

Abstract

The spread and development of antibiotic resistance globally have led to severe public health problems. It has been shown that some non-antibiotic substances can also promote the diffusion and spread of antibiotic resistance genes (ARGs). Nanofullerene (nC₆₀) is a type of nanomaterial widely used around the world, and some studies have discovered both the biological toxicity and environmental toxicity of nC₆₀. In this study, cellular and molecular biology techniques were employed to investigate the influences of nC₆₀ at sub-minimum inhibitory concentrations (sub-MICs) on the conjugation of ARGs between the E. coli strains. Compared with the control group, nC₆₀ significantly increased the conjugation rates of ARGs by 1.32‒10.82 folds within the concentration range of 7.03‒1800 mg/L. This study further explored the mechanism of this phenomenon, finding that sub-MICs of nC₆₀ could induce the production of reactive oxygen species (ROS), trigger SOS-response and oxidative stress, affect the expression of outer membrane proteins (OMPs) genes, increase membrane permeability, and thus promote the occurrence of conjugation. This research enriches our understanding of the environmental toxicity of nC₆₀, raises our risk awareness toward nC₆₀, and may promote the more rational employment of nC₆₀ materials.

Graphical abstract

Keywords

Nanofullerene / Sub-minimum inhibitory concentrations / Antibiotic resistance genes / Conjugation / Molecular biological techniques

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 91843301, 21527814 and 91643106).

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-020-1287-0 and is accessible for authorized users.

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