Aerobic granular sludge formation based on substrate availability: Effects of flow pattern and fermentation pretreatment

Abstract

• Penetration depth and substrate characters affect AGS formation and performance.

• The relationship between substrate gradient and particle size affects AGS stability.

• The fermentation process is proposed as a pretreatment to improve AGS stability.

The influences of flow patterns (mixed-flow and plug-flow) and fermentation pretreatment on aerobic granular sludge (AGS) formation with various substrate availability levels were investigated by running four identical laboratory-scale sequencing batch reactors (R1–R4), comparing two anaerobic feeding strategies and three kinds of substrates. R1 achieved faster granulation with a fast influent fill step followed by a modified anaerobic mixed-flow phase, but the AGS showed poorer stability with a cracked structure and a high suspended solids concentration in the effluent. The anaerobic plug-flow feeding mode (with influent fed slowly from the bottom) in R2 provided deeper penetration depth for the substance to reach the core of AGS and accordingly strengthen AGS stability. An acidogenic up-flow sludge bed reactor was introduced as a pretreatment to improve the AGS performance by enhancing glucose pre-fermentation (R4). AGS fed with mixed volatile fatty acids (VFA) after glucose fermentation showed similar performance compared with the reactor fed with acetate in the aspects of stability, structure, size distribution and nitrogen removal efficiency, and 74% similarity in the microbial community. For actual wastewater with low VFA concentrations, fermentation treatment was suggested as a promising pretreatment for stable AGS granulation and operation.

Graphical abstract

Keywords

Anaerobic plug-flow feeding mode / Anaerobic mixed-flow mode / Fermentation pretreatment / Substrate gradient / Penetration depth

References

[1] Adav S S, Lee D J, Show K Y, Tay J H (2008). Aerobic granular sludge: Recent advances. Biotechnology Advances, 26(5): 411–423

[2] Amorim C L, Maia A S, Mesquita R B R, Rangel A N O S S, Loosdrecht M C M V, Tiritan M E, Castro P M L (2014). Performance of aerobic granular sludge in a sequencing batch bioreactor exposed to ofloxacin, norfloxacin and ciprofloxaci. Water Research, 50: 101–113

[3] APHA (1998). Standard Methods for the Examination of Water and Wastewater, 20th ed. Washington, DC: American Public Health Association/American Water Works Association/Water Environment Federation

[4] Beun J J, Loosdrecht M C M V, Heijnen J J (2002). Aerobic granulation in a sequencing batch airlift reactor. Water Research, 36(3): 702–712

[5] Beun J J, Verhoef E V,Loosdrecht M C M V, Heijnen J J (2000). Stoichiometry and kinetics of poly-beta-hydroxybutyrate metabolism under denitrifying conditions in activated sludge cultures. Biotechnology and Bioengineering, 68(5): 496–507

[6] Caporaso J G, Kuczynski J, Stombaugh J, Bittinger K, Bushman F D, Costello E K, Fierer N, Peña A G, Goodrich J K, Gordon J I, Huttley G A, Kelley S T, Knights D, Koenig J E, Ley R E, Lozupone C A, Mcdonald D, Muegge B D, Pirrung M, Reeder J, Sevinsky J R, Turnbaugh P J, Walters W A, Widmann J, Yatsunenko T, Zaneveld J, Knight R (2010). QIIME allows analysis of high-throughput community sequencing data. Nature Methods, 7(5): 335–336

[7] Cydzik-Kwiatkowska A (2015). Bacterial structure of aerobic granules is determined by aeration mode and nitrogen load in the reactor cycle. Bioresource Technology, 181: 312–320

[8] He Q, Song Q, Zhang S, Zhang W, Wang H (2018). Simultaneous nitrification, denitrification and phosphorus removal in an aerobic granular sequencing batch reactor with mixed carbon sources: reactor performance, extracellular polymeric substances and microbial successions. Chemical Engineering Journal, 331: 841–849

[9] Kreuk M K D, Loosdrecht M C M V (2004). Selection of slow growing organisms as a means for improving aerobic granular sludge stability. Water Science and Technology, 49(11–12): 9–17

[10] Kreuk M K D, Heijnen J J, Loosdrecht M C M V (2005). Simultaneous COD, nitrogen, and phosphate removal by aerobic granular sludge. Biotechnology and Bioengineering, 90(6): 761–769

[11] Kreuk M K, Kishida N, Loosdrecht M C MV( 2007). Aerobic granular sludge – state of the art. Water Science and Technology, 55(8–9): 75–81

[12] Lee D J, Chen Y Y, Show K Y, Whiteley C G, Tay J H (2010). Advances in aerobic granule formation and granule stability in the course of storage and reactor operation. Biotechnology Advances, 28(6): 919–934

[13] Liu J, Li J, Wang X, Zhang Q, Littleton H (2017). Rapid aerobic granulation in an SBR treating piggery wastewater by seeding sludge from a municipal WWTP. Journal of Environmental Sciences-China, 51: 332–341

[14] Morgenroth E, Sherden T, Loosdrecht M C M V, Heijnen J J, Wilderer P A (1997). Aerobic granular sludge in a sequencing batch reactor. Water Research, 31(12): 3191–3194

[15] Nowka B, Daims H, Spieck E (2015). Comparison of oxidation kinetics of nitrite-oxidizing bacteria: Nitrite availability as a key factor in niche differentiation. Applied and Environmental Microbiology, 81(2): 745–753

[16] Picioreanu C, Loosdrecht M C M V, Heijnen J (1998). Mathematical modeling of biofilm structure with a hybrid differential-discrete cellular automaton approach. Biotechnology and Bioengineering, 58(1): 101–116

[17] Pronk M, Abbas B, Al-Zuhairy S H, Kraan R, Kleerebezem R, Loosdrecht M C M V (2015). Effect and behaviour of different substrates in relation to the formation of aerobic granular sludge. Applied Microbiology and Biotechnology, 99(12): 5257–5268

[18] Reino C, Suarez-Ojeda M E, Perez J, Carrera J (2016). Kinetic and microbiological characterization of aerobic granules performing partial nitritation of a low-strength wastewater at 10°C. Water Research, 101: 147–156

[19] Rocktäschel T, Klarmann C, Helmreich B, Ochoa J, Boisson P, Sorensen K H, Horn H (2013). Comparison of two different anaerobic feeding strategies to establish a stable aerobic granulated sludge bed. Water Research, 47(17): 6423–6431

[20] Show K Y, Lee D J, Tay J H (2012). Aerobic granulation: Advances and challenges. Applied Biochemistry and Biotechnology, 167(6): 1622–1640

[21] Sinclair L, Osman O A, Bertilsson S, Eiler A (2015). Microbial community composition and diversity via 16S rRNA gene amplicons: Evaluating the illumina platform. PLoS One, 10(2): e0116955

[22] Sun H, Yu P, Li Q, Ren H, Liu B, Ye L, Zhang X X (2017). Transformation of anaerobic granules into aerobic granules and the succession of bacterial community. Applied Microbiology and Biotechnology, 101(20): 7703–7713

[23] Tay J H, Liu Q S, Liu Y (2001). The effects of shear force on the formation, structure and metabolism of aerobic granules. Applied Microbiology and Biotechnology, 57 (1–2): 227–233

[24] Tay J H, Liu Q S, Liu Y (2002). Characteristics of aerobic granules grown on glucose and acetate in sequential aerobic sludge blanket reactors. Environmental Technology, 23(8): 931–936

[25] Tijhuis L, Loosdrecht M C M V, Heijnen J J (1994). Formation and growth of heterotrophic aerobic biofilms on small suspended particles in airlift reactors. Biotechnology and Bioengineering, 44(5): 595–608

[26] Wagner J, Weissbrodt D G, Manguin V, Ribeiro da Costa R H, Morgenroth E, Derlon N (2015). Effect of particulate organic substrate on aerobic granulation and operating conditions of sequencing batch reactors. Water Research, 85: 158–166

[27] Wang L, Deng S, Wang S, Su H (2017). Analysis of aerobic granules under the toxic effect of ampicillin in sequencing batch reactors: Performance and microbial community. Journal of Environmental Management, 204(1): 152–159

[28] Wang X, Hu M, Xia Y, Wen X, Ding K (2012). Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China. Applied and Environmental Microbiology, 78(19): 7042–7047

[29] Xing X, Yu Y, Xi H, Song G, Wang Y, Zuo J, Zhou Y (2018). Reduction of wastewater toxicity and change of microbial community in a hydrolysis acidification reactor pre-treating trimethylolpropane wastewater. Frontiers of Environmental Science & Engineering, 12(6): 12

[30] Yuan Q, Gong H, Xi H, Xu H, Jin Z, Ali N, Wang K (2019). Strategies to improve aerobic granular sludge stability and nitrogen removal based on feeding mode and substrate. Journal of Environmental Sciences (China), 84: 144–154

[31] Zhang Q, Hu J, Lee D J (2016). Aerobic granular processes: Current research trends. Bioresource Technology, 210: 74–80

[32] Zheng M, Zheng M, Wu Y, Ma H, Wang K (2015). Effect of pH on types of acidogenic fermentation of fruit and vegetable wastes. Biotechnology and Bioprocess Engineering, 20(2): 298–303

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