Comparative genotoxicity of water processed by three drinking water treatment plants with different water treatment procedures

Abstract

• Genotoxicity of substances is unknown in the water after treatment processes.

• Genotoxicity decreased by activated carbon treatment but increased by chlorination.

• Halogenated hydrocarbons and aromatic compounds contribute to genotoxicity.

• Genotoxicity was assessed by umu test; acute and chronic toxicity by ECOSAR.

• Inconsistent results confirmed that genotoxicity cannot be assessed by ECOSAR.

Advanced water treatment is commonly used to remove micropollutants such as pesticides, endocrine disrupting chemicals, and disinfection byproducts in modern drinking water treatment plants. However, little attention has been paid to the changes in the genotoxicity of substances remaining in the water following the different water treatment processes. In this study, samples were collected from three drinking water treatment plants with different treatment processes. The treated water from each process was analyzed and compared for genotoxicity and the formation of organic compounds. The genotoxicity was evaluated by an umu test, and the acute and chronic toxicity was analyzed through Ecological Structure- Activity Relationship (ECOSAR). The results of the umu test indicated that biological activated carbon reduced the genotoxicity by 38%, 77%, and 46% in the three drinking water treatment plants, respectively, while chlorination increased the genotoxicity. Gas chromatograph-mass spectrometry analysis revealed that halogenated hydrocarbons and aromatic compounds were major contributors to genotoxicity. The results of ECOSAR were not consistent with those of the umu test. Therefore, we conclude that genotoxicity cannot be determined using ECOSAR .

Graphical abstract

Keywords

Drinking water / Treatment process / Genotoxicity / Umu test / Ecological Structure-Activity Relationship

References

[1] Ao X, Liu W , Sun W, Cai M, Ye Z, Yang C, Li C (2018). Medium pressure UV-activated peroxymonosulfate for ciprofloxacin degradation: Kinetics, mechanism, and genotoxicity. Chemical Engineering Journal, 345: 87–97

[2] Brinkmann C, Eisentraeger A (2008). Completely automated short-term genotoxicity testing for the assessment of chemicals and characterisation of contaminated soils and waste waters. Environmental Science and Pollution Research International, 15(3): 211–217

[3] Chai Q W, Hu A, Qian Y K, Ao X W, Liu W J, Yang H W, Xie Y F (2018). A comparison of genotoxicity changes in reclaimed wasterwater from different disinfection processes. Chemosphere, 191: 335–341

[4] Chen T, Xu Y, Liu Z, Zhu S, Shi W, Cui F (2016). Evaluation of drinking water treatment combined filter backwash water recycling technology based on comet and micronucleus assay. Journal of Environmental Sciences (China), 42: 61–70

[5] Cheng X, Chen L, Sun R, Jing Y (2019). Identification of regional water resource stress based on water quantity and quality: A case study in a rapid urbanization region of China. Journal of Cleaner Production, 209: 216–223

[6] Chow C W K, Van Leeuwen J A, Drikas M, Fabris R, Spark K M, Page D W (1999). The impact of the character of natural organic matter in conventional treatment with alum. Water Science and Technology, 40(9): 97–104

[7] De Jesus Gaffney V, Almeida C M M, Rodrigues A, Ferreira E, Benoliel M J, Cardoso V V(2015). Occurrence of pharmaceuticals in a water supply system and related human health risk assessment. Water Research, 72: 199–208

[8] Esplugas S, Bila D M, Krause L G T, Dezotti M (2007). Ozonation and advanced oxidation technologies to remove endocrine disrupting chemicals (EDCs) and pharmaceuticals and personal care products (PPCPs) in water effluents. Journal of Hazardous Materials, 149(3): 631–642

[9] Gert-Jan, De Maagd, Tonkes (2000). Selection of genotoxicity tests for risk assessment of effluents. Environ. Toxicol. 15(2): 81–90

[10] Giuliani T , Koller T, Würgler F E, Widmer R M (1996). Detection of genotoxic activity in native hospital waste water by the umuC test. Mutation Research/Genetic Toxicology, 368(1): 49–57

[11] Grimmett P E, Munch J W(2013). Development of EPA Method 525.3 for the analysis of semivolatiles in drinking water. Analytical Methods, 5(1): 151–163

[12] Hamer B, Bihari N, Reifferscheid G, Zahn R, Müller W E G, Batel R(2000). Evaluation of the SOS/umu-test post-treatment assay for the detection of genotoxic activities of pure compounds and complex environmental mixtures. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 466(2): 161–171

[13] Han Y, Li N, Oda Y, Ma M, Rao K, Wang Z, Jin W, Hong G, Li Z, Luo Y (2016). Evaluation of genotoxic effects of surface waters using a battery of bioassays indicating different mode of action. Ecotoxicology and Environmental Safety, 133: 448–456

[14] Han Y, Ma M, Li R, Hou C, Huang Y, Oda Y, Wang Z (2018). Chlorination, chloramination and ozonation of carbamazepine enhance cytoxicity and genotoxicity: Multi-endpoint evaluation and identification of its genotoxic transformation products. Journal of Hazardous Materials, 342: 679–688

[15] Kameya , Nagato , Nakagawa , Yamashita , Kobayashi , Fujie (2011). Quantification of umu genotoxicity level of urban river water. Water Science and Technology, 63(3): 410–415

[16] Kapelewska J, Kotowska U, Karpińska J, Kowalczuk D, Arciszewska A, Świrydo A (2018). Occurrence, removal, mass loading and environmental risk assessment of emerging organic contaminants in leachates, ground waters and wastewaters. Microchemical Journal, 137: 292–301

[17] Klüver N, Bittermann K, Escher B I (2019). ECOSAR for baseline toxicity and classification of specific modes of action of ionizable organic chemicals in the zebrafish embryo toxicity test. Aquatic Toxicology (Amsterdam, Netherlands), 207: 110–119

[18] Koivusalo M, Pukkala E, Vartiainen T, Jaakkola J J K, Hakulinen T (1997). Drinking water chlorination and cancer: A historical cohort study in Finland. Cancer Causes & Control, 8(2): 192–200

[19] Koivusalo M, Vartiainen T (1997). Drinking water chlorination by-products and cancer. Reviews on Environmental Health, 12(2): 81–90

[20] Li D, Liu S (2019). Water Quality Monitoring and Management. London: Academic Press

[21] Li L, Zhang P, Zhu W, Han W, Zhang Z (2005). Comparison of O₃-BAC, UV/O₃-BAC and TiO₂/UV/O₃-BAC processes for removing organic pollutants in secondary effluents. Journal of Photochemistry and Photobiology A Chemistry, 171(2): 145–151

[22] Li L, Zhu W, Zhang P, Lu P, Zhang Q, Zhang Z (2007). UV/O₃-BAC process for removing organic pollutants in secondary effluents. Desalination, 207(1–3): 114–124

[23] Li M, Wei D, Du Y (2016a). Genotoxicity of quinolone antibiotics in chlorination disinfection treatment: formation and ECOSAR simulation. Environmental Science and Pollution Research International, 23(20): 20637–20645

[24] Li W, Wu R, Duan J, Saint C P, van Leeuwen J (2016b). Impact of prechlorination on organophosphorus pesticides during drinking water treatment: Removal and transformation to toxic oxon byproducts. Water Research, 105: 1–10

[25] Liu J L, Wong M H (2013). Pharmaceuticals and personal care products (PPCPs): A review on environmental contamination in China. Environment International, 59: 208–224

[26] Liu W J, Gao J W. (2009). Study on the Genotoxicity of Source Water and Drinking Water During Chlorine and Chloramine Disinfection Using the Umu-Test. Progress in Environmental Science and Technology, Vol Ii, Pts A and B, ed. S.C. Li, et al. 283–288

[27] Liu Y, He X, Duan X, Fu Y, Dionysiou D D (2015). Photochemical degradation of oxytetracycline: Influence of pH and role of carbonate radical. Chemical Engineering Journal, 276: 113–121

[28] Liviac D, Wagner E D, Mitch W A, Altonji M J, Plewa M J (2010). Genotoxicity of water concentrates from recreational pools after various disinfection methods. Environmental Science & Technology, 44(9): 3527–3532

[29] Mohd Zainudin F, Abu Hasan H, Sheikh Abdullah S R. (2018). An overview of the technology used to remove trihalomethane (THM), trihalomethane precursors, and trihalomethane formation potential (THMFP) from water and wastewater. Journal of Industrial and Engineering Chemistry, 57: 1–14

[30] Nie X, Liu W, Zhang L, Liu Q (2017). Genotoxicity of drinking water treated with different disinfectants and effects of disinfection conditions detected by umu-test. Journal of Environmental Sciences (China), 56: 36–44

[31] Oda Y (2017). Advanced Approaches to Model Xenobiotic Metabolism in Bacterial Genotoxicologyin vitro. Cham: Springer

[32] Oda Y, Funasaka K, Kitano M, Nakama A, Yoshikura T (2004). Use of a high‐throughput umu‐microplate test system for rapid detection of genotoxicity produced by mutagenic carcinogens and airborne particulate matter. Environmental and Molecular Mutagenesis, 43(1): 10–19

[33] Okunola A A, Babatunde E E, Chinwe D, Pelumi O, Ramatu S G (2016). Mutagenicity of automobile workshop soil leachate and tobacco industry wastewater using the Ames Salmonella fluctuation and the SOS chromotests. Toxicology and Industrial Health, 32(6): 1086–1096

[34] Pulliero A, Cao J, Vasques L R, Pacchierotti F(2015). Genetic and epigenetic effects of environmental mutagens and carcinogens. BioMed Research International, 2015: 1

[35] Reifferscheid G, Arndt C, Schmid C (2005). Further development of the β-lactamase MutaGen assay and evaluation by comparison with Ames fluctuation tests and the umu test. Environmental and Molecular Mutagenesis, 46(2): 126–139

[36] Richardson S D, Plewa M J, Wagner E D, Schoeny R, DeMarini D M (2007). Occurrence, genotoxicity, and carcinogenicity of regulated and emerging disinfection by-products in drinking water: A review and roadmap for research. Mutation Research/Reviews in Mutation Research, 636(1–3): 178–242

[37] Rimayi C, Odusanya D, Weiss J M, de Boer J, Chimuka L (2018). Contaminants of emerging concern in the Hartbeespoort Dam catchment and the Umngeni River estuary 2016 pollution incident, South Africa. Science of the Total Environment, 627: 1008–1017

[38] Sgroi M, Vagliasindi F G A, Snyder S A, Roccaro P (2018). N-Nitrosodimethylamine (NDMA) and its precursors in water and wastewater: A review on formation and removal. Chemosphere, 191: 685–703

[39] Shao S, Wang Y, Shi D, Zhang X, Tang C Y, Liu Z, Li J (2018). Biofouling in ultrafiltration process for drinking water treatment and its control by chlorinated-water and pure water backwashing. Science of the Total Environment, 644: 306–314

[40] Suzuki T, Kitamura S, Khota R, Sugihara K, Fujimoto N, Ohta S (2005). Estrogenic antiandrogenic activities of 17 benzophenone derivatives used as UV stabilizers and sunscreens. Toxicology and Applied Pharmacology, 203(1): 9–17

[41] Toolaram A P, Gutierrez I R, Ahlf W (2012). Modification of the umu-assay (ISO 13829) accounting for cytotoxicity in genotoxicity assessment: A preliminary study. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 747(2): 190–196

[42] UN (2011), Globally Harmonized System of Classification and Labelling of Chemical (GHS). New York: United Nations Publications

[43] Vughs D, Baken K A, Kolkman A, Martijin A J, Voogt P D (2018). Application of effect-directed analysis to identify mutagenic nitrogenous disinfection by-products of advanced oxication drinking water treatment. Environmental Science and Pollution Research International, 25(5): 3951–3964

[44] Wang L S, Hu H Y, Ta C H, Tian J, Wang C, Koichi F (2007). Change in genotoxicity of wastewater during chlorine dioxide and chlorine disinfections and the influence of ammonia nitrogen. Chin. J.Environ. Sci, 28(3): 603–666

[45] Wang L S, Zhang T, Hu H Y (2005). Comparisom of the quality and toxicity of wastewater after chlorine and chlorine dioxide disinfections. Chin. J. Environ. Sci, 26(6): 75–78

[46] Wang X J, Hu X X, Hu C, Wei D B (2011). Sequentiald use of ultraviolet light and chlorine for reclaimed water disinfection. Journal of Environmental Sciences (China), 23(10): 1605–1610

[47] Xie Y F, Zhou H J (2002). Use of BAC for HAA removal–Part2, Column study. Journal- American Water Works Association, 94(5): 126–134

[48] Yang C, Sun W J, Ao X W (2019). Using mRNA to investigate the effect of low-pressure ultraviolet disinfection on the viability of E.coli. Frontiers of Environmental Science & Engineering, 13(2): 2095–2201

[49] Yang W, Zhou H, Cicek N (2014). Treatment of organic micropollutants in water and wastewater by UV-based processes: A literature review. Critical Reviews in Environmental Science and Technology, 44(13): 1443–1476

[50] Zha X, Ma L, Wu J, Liu Y (2016). The removal of organic precursors of DBPs during three advanced water treatment processes including ultrafiltration, biofiltration, and ozonation. Environmental Science and Pollution Research International, 23(16): 16641–16652

[51] Zhang Y, Li S, Fang Q, Duan Y, Ou P, Wang L, Chen Z, Wang F (2019). Implementation of long-term assessment of human health risk for metal contaminated groundwater: A coupled chemical mass balance and hydrodynamics model. Ecotoxicology and Environmental Safety, 180: 95–105

[52] Zhao Z, Sun W, Ray M B, Ray A K, Huang T, Chen J. (2019). Optimization and modeling of coagulation-flocculation to remove algae and organic matter from surface water by response surface methodology. Frontiers of Environmental Science & Engineering, 13(5): 75-90

[53] Zhu Z, Gu WM, An W, Hu J Y (2008), Carcinogen risk assessment of drinking water based on genotoxic activities using SOS/umu test. Asian Journal of Ecotoxicology. 4, 363–369

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