The impact of ultrasonic treatment on activity of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in activated sludge

Abstract

Conditions for ultrasonic treatment to achieve partial nitritation are optimized.

Ultrasound reduces metabolic activity and releases intracellular metabolites.

Mechanical shearing is essential to inhibit nitrite oxidation.

The ultrasonic treatment of sludge has been considered as an effective method to facilitate the partial nitritation of municipal sewage. This study aims to reveal the effects of ultrasound on ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB). The impact factors including ultrasonic irradiation time and intensity, sludge concentration, thermal effect and released free radicals were studied. The maximized difference between the changes in AOB and NOB activities were obtained with 10 g mixed liquor suspended solids (MLSS)/L, using 0.9 kJ/mL ultrasonic energy density and 12 h interval time. The increased ultrasonic intensity destroyed the floc structure of activated sludge, increased the microbial death, and decreased the cellular ATP level. Further, the mechanism exploration indicated that the mechanical shearing could be a critical factor in achieving the nitritation with inhibitory effect on nitrite oxidation.

Graphical abstract

Keywords

Ultrasonic treatment / Optimal control / Nitrifying bacteria / Mechanism analysis

References

[1] Bashari M, Eibaid A, Wang J, Tian Y, Xu X, Jin Z (2013). Influence of low ultrasound intensity on the degradation of dextran catalyzed by dextranase. Ultrasonics Sonochemistry, 20(1): 155–161

[2] Blackburne R, Yuan Z, Keller J (2008). Demonstration of nitrogen removal via nitrite in a sequencing batch reactor treating domestic wastewater. Water Research, 42(8–9): 2166–2176

[3] Cao Y, van Loosdrecht M C M, Daigger G T (2017). Mainstream partial nitritation-anammox in municipal wastewater treatment: Status, bottlenecks, and further studies. Applied Microbiology and Biotechnology, 101(4): 1365–1383

[4] Chisti Y (2003). Sonobioreactors: Using ultrasound for enhanced microbial productivity. Trends in Biotechnology, 21(2): 89–93

[5] Ciccolini L, Taillandier P, Wilhem A M, Delmas H, Strehaiano P (1997). Low frequency thermo-ultrasonication of Saccharomyces cerevisiae suspensions: Effect of temperature and of ultrasonic power. Chemical Engineering Journal, 65(2): 145–149

[6] Gao D, Peng Y, Wu W M (2010). Kinetic model for biological nitrogen removal using shortcut nitrification-denitrification process in sequencing batch reactor. Environmental Science & Technology, 44(13): 5015–5021

[7] Gao S, Hemar Y, Ashokkumar M, Paturel S, Lewis G D (2014a). Inactivation of bacteria and yeast using high-frequency ultrasound treatment. Water Research, 60: 93–104

[8] Gao S, Lewis G D, Ashokkumar M, Hemar Y (2014b). Inactivation of microorganisms by low-frequency high-power ultrasound: 1. Effect of growth phase and capsule properties of the bacteria. Ultrasonics Sonochemistry, 21(1): 446–453

[9] Guerrero S, López-Malo A, Alzamora S M (2001). Effect of ultrasound on the survival of Saccharomyces cerevisiae: Influence of temperature, pH and amplitude. Innovative Food Science & Emerging Technologies, 2(1): 31–39

[10] Han Z, Miao Y, Dong J, Shen Z, Zhou Y, Liu S, Yang C (2019). Enhanced nitrogen removal and microbial analysis in partially saturated constructed wetland for treating anaerobically digested swine wastewater. Frontiers of Environmental Science & Engineering, 13(4): 52

[11] Huang G, Chen S, Dai C, Sun L, Sun W, Tang Y, Xiong F, He R, Ma H (2017). Effects of ultrasound on microbial growth and enzyme activity. Ultrasonics Sonochemistry, 37: 144–149

[12] Liu Y, Li X, Kang X, Yuan Y, Du M (2014). Short chain fatty acids accumulation and microbial community succession during ultrasonic-pretreated sludge anaerobic fermentation process: Effect of alkaline adjustment. International Biodeterioration & Biodegradation, 94: 128–133

[13] Ma Y, Peng Y, Wang S, Yuan Z, Wang X (2009). Achieving nitrogen removal via nitrite in a pilot-scale continuous pre-denitrification plant. Water Research, 43(3): 563–572

[14] Marchesini G, Fasolato L, Novelli E, Balzan S, Contiero B, Montemurro F, Andrighetto I, Segato S (2015). Ultrasonic inactivation of microorganisms: A compromise between lethal capacity and sensory quality of milk. Innovative Food Science & Emerging Technologies, 29: 215–221

[15] Nickel K, Neis U (2007). Ultrasonic disintegration of biosolids for improved biodegradation. Ultrasonics Sonochemistry, 14(4): 450–455

[16] Peng Y, Zhu G (2006). Biological nitrogen removal with nitrification and denitrification via nitrite pathway. Applied Microbiology and Biotechnology, 73(1): 15–26

[17] Pitt W G, McBride M O, Lunceford J K, Roper R J, Sagers R D (1994). Ultrasonic enhancement of antibiotic action on gram-negative bacteria. Antimicrobial Agents and Chemotherapy, 38(11): 2577–2582

[18] Piyasena P, Mohareb E, McKellar R C (2003). Inactivation of microbes using ultrasound: A review. International Journal of Food Microbiology, 87(3): 207–216

[19] Quan X, Huang K, Li M, Lan M, Li B (2018). Nitrogen removal performance of municipal reverse osmosis concentrate with low C/N ratio by membrane-aerated biofilm reactor. Frontiers of Environmental Science & Engineering, 12(6): 5

[20] Raso J, Pagán R, Condón S, Sala F J (1998). Influence of temperature and pressure on the lethality of ultrasound. Applied and Environmental Microbiology, 64(2): 465–471

[21] Rokhina E V, Lens P, Virkutyte J (2009). Low-frequency ultrasound in biotechnology: State of the art. Trends in Biotechnology, 27(5): 298–306

[22] Song X, Liu R, Chen L, Dong B, Kawagishi T (2017). Advantages of intermittently aerated SBR over conventional SBR on nitrogen removal for the treatment of digested piggery wastewater. Frontiers of Environmental Science & Engineering, 11(3): 13

[23] Song Y, Hahn T, Thompson I P, Mason T J, Preston G M, Li G, Paniwnyk L, Huang W E (2007). Ultrasound-mediated DNA transfer for bacteria. Nucleic Acids Research, 35(19): e129

[24] Subhedar P B, Gogate P R (2014). Enhancing the activity of cellulase enzyme using ultrasonic irradiations. Journal of Molecular Catalysis. B, Enzymatic: 101: 108–114

[25] Turk O, Mavinic D S (1986). Preliminary assessment of a shortcut in nitrogen removal from wastewater. Canadian Journal of Civil Engineering, 13(6): 600–605

[26] Wang M, Li R, Zhao Q (2019). Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline, thermal hydrolysis and ultrasonic pretreatments. Frontiers of Environmental Science & Engineering, 13(3): 43

[27] Wang Q, Kuninobu M, Kakimoto K, I-Ogawa H, Kato Y (1999). Upgrading of anaerobic digestion of waste activated sludge by ultrasonic pretreatment. Bioresource Technology, 68(3): 309–313

[28] Wu S, Zheng M, Dong Q, Liu Y, Wang C (2018). Evaluating the excess sludge reduction in activated sludge system with ultrasonic treatment. Water Science & Technology, 77(9–10): 2341–2347

[29] Yang Q, Liu X, Peng C, Wang S, Sun H, Peng Y (2009). N₂O production during nitrogen removal via nitrite from domestic wastewater: Main sources and control method. Environmental Science & Technology, 43(24): 9400–9406

[30] Zheng M, Duan H, Dong Q, Ni B J, Hu S, Liu Y, Huang X, Yuan Z (2019a). Effects of ultrasonic treatment on the ammonia-oxidizing bacterial (AOB) growth kinetics. Science of the Total Environment, 690: 629–635

[31] Zheng M, Liu Y C, Xin J, Zuo H, Wang C W, Wu W M (2016). Ultrasonic treatment enhanced ammonia-oxidizing bacterial (AOB) activity for nitritation process. Environmental Science & Technology, 50(2): 864–871

[32] Zheng M, Liu Y C, Xu K N, Wang C W, He H, Zhu W, Dong Q (2013). Use of low frequency and density ultrasound to stimulate partial nitrification and simultaneous nitrification and denitrification. Bioresource Technology, 146: 537–542

[33] Zheng M, Wu S, Dong Q, Huang X, Yuan Z, Liu Y (2019b). Achieving mainstream nitrogen removal via the nitrite pathway from real municipal wastewater using intermittent ultrasonic treatment. Ultrasonics Sonochemistry, 51: 406–411

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