Reconsideration on the effect of nitrogen on mixed culture polyhydroxyalkanoate production toward high organic loading enrichment history
Zhiqiang Chen, Lizhi Zhao, Ye Ji, Qinxue Wen, Long Huang
Reconsideration on the effect of nitrogen on mixed culture polyhydroxyalkanoate production toward high organic loading enrichment history
Effect of nitrogen on mixed culture PHA production was reconsidered.
Enrichment history of PHA accumulating culture was discussed.
Higher PHA content and biomass growth were achieved in presence of nitrogen.
Enrichment strategy toward higher PHA accumulation was investigated.
Microbial community succession in PHA accumulation phase was investigated.
In most of the operating strategies for mixed microbial cultures polyhydroxyalkanoate (PHA) production, moderate organic loads and low nitrogen concentrations are used, however, the real waste streams contain variable concentrations of carbon and nitrogen. To evaluate the effect of enrichment history on PHA producer and production the various carbon and nitrogen levels were utilized during the accumulation phase. Different operating strategies were applied in three sequencing batch reactors (SBRs) subjected to aerobic dynamic feeding. The maximum PHA production of the enriched cultures under nutrient excess, limitation and starvation (Cmol/Nmol ratio of 8, 40 and ∞, respectively) was evaluated in batch assays. A higher PHA content and biomass growth were achieved in the nutrients presence in comparison to the nutrient starvation condition. The cultures from the SBR treated under short sludge retention time, high organic loading rate, short cycle length (SBR#3) and nutrient excess reached the maximum PHA content (54.9%) and biomass increase (38.9%). Under nutrient limitation, the negative biomass growth was observed under nutrient starvation because of the sampling loss. The succession of microbial communities in SBRs and batch assays was analyzed using terminal restriction fragment length polymorphism. The SBR#3 had the best overall PHA production performance considering its high PHA content and productivity in all nutrient content, it indicates that nitrogen has a substantial impact on PHA yield especially when high organic loading rate enrichment history is involved.
Polyhydroxyalkanoate (PHA) / Organic loading rate / Nitrogen content / Biomass growth / Enrichment history
[1] |
Albuquerque M G E, Carvalho G, Kragelund C, Silva A F, Barreto Crespo M T, Reis M A M, Nielsen P H (2013). Link between microbial composition and carbon substrate-uptake preferences in a PHA-storing community. The ISME Journal, 7(1): 1–12
CrossRef
Pubmed
Google scholar
|
[2] |
Albuquerque M G E, Eiroa M, Torres C, Nunes B R, Reis M A M (2007). Strategies for the development of a side stream process for polyhydroxyalkanoate (PHA) production from sugar cane molasses. Journal of Biotechnology, 130(4): 411–421
CrossRef
Pubmed
Google scholar
|
[3] |
Bengtsson S, Werker A, Christensson M, Welander T (2008). Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater. Bioresource Technology, 99(3): 509–516
CrossRef
Pubmed
Google scholar
|
[4] |
Cai M, Chua H, Zhao Q, Sin N, Ren J (2009). Optimal production of polyhydroxyalkanoates (PHA) in activated sludge fed by volatile fatty acids (VFAs) generated from alkaline excess sludge fermentation. Bioresource Technology, 100(3): 1399–1405
Pubmed
|
[5] |
Chen Z, Huang L, Wen Q, Guo Z (2015). Efficient polyhydroxyalkanoate (PHA) accumulation by a new continuous feeding mode in three-stage mixed microbial culture (MMC) PHA production process. Journal of Biotechnology, 209: 68–75
CrossRef
Pubmed
Google scholar
|
[6] |
Chung K Y, Han S S, Kim H K, Choi G S, Kim I S, Lee S S, Woo S H, Lee K H, Kim J J (2006). Inhibitory effect of the selected heavy metals on the growth of the phosphorus accumulating microorganism, Acinetobacter sp. Korean Journal of Environmental Agriculture, 25(1): 40–46
CrossRef
Google scholar
|
[7] |
Dionisi D, Beccari M, Di Gregorio S, Majone M, Papini M P, Vallini G (2005). Storage of biodegradable polymers by an enriched microbial community in a sequencing batch reactor operated at high organic load rate. Journal of Chemical Technology & Biotechnology Biotechnology, 80(11): 1306–1318
CrossRef
Google scholar
|
[8] |
Dionisi D, Majone M, Papa V, Beccari M (2004). Biodegradable polymers from organic acids by using activated sludge enriched by aerobic periodic feeding. Biotechnology and Bioengineering, 85(6): 569–579
CrossRef
Pubmed
Google scholar
|
[9] |
Dionisi D, Majone M, Vallini G, Di Gregorio S, Beccari M (2006). Effect of the applied organic load rate on biodegradable polymer production by mixed microbial cultures in a sequencing batch reactor. Biotechnology and Bioengineering, 93(1): 76–88
CrossRef
Pubmed
Google scholar
|
[10] |
Divyashree M S, Rastogi N K, Shamala T R (2009). A simple kinetic model for growth and biosynthesis of polyhydroxyalkanoate in Bacillus flexus. New Biotechnology, 26(1–2): 92–98
CrossRef
Pubmed
Google scholar
|
[11] |
Gurieff N, Lant P (2007). Comparative life cycle assessment and financial analysis of mixed culture polyhydroxyalkanoate production. Bioresource Technology, 98(17): 3393–3403
CrossRef
Pubmed
Google scholar
|
[12] |
Hao J, Wang X, Wang H (2017). Investigation of polyhydroxyalkanoates (PHAs) biosynthesis from mixed culture enriched by valerate-dominant hydrolysate. Frontiers of Environmental Science & Engineering, 11(1): 5 doi.org/10.1007/s11783-017-0896-8
|
[13] |
Huang L, Chen Z, Wen Q, Zhao L, Lee D J, Yang L, Wang Y (2018). Insights into Feast-Famine polyhydroxyalkanoate (PHA)-producer selection: Microbial community succession, relationships with system function and underlying driving forces. Water Research, 131: 167–176
CrossRef
Pubmed
Google scholar
|
[14] |
Inoue D, Suzuki Y, Uchida T, Morohoshi J, Sei K (2016). Polyhydroxyalkanoate production potential of heterotrophic bacteria in activated sludge. Journal of Bioscience and Bioengineering, 121(1): 47–51
CrossRef
Pubmed
Google scholar
|
[15] |
Jia Q, Xiong H, Wang H, Shi H, Sheng X, Sun R, Chen G (2014). Production of polyhydroxyalkanoates (PHA) by bacterial consortium from excess sludge fermentation liquid at laboratory and pilot scales. Bioresource Technology, 171: 159–167
CrossRef
Pubmed
Google scholar
|
[16] |
Jiang Y, Chen Y, Zheng X (2009). Efficient polyhydroxyalkanoates production from a waste-activated sludge alkaline fermentation liquid by activated sludge submitted to the aerobic feeding and discharge process. Environmental Science & Technology, 43(20): 7734–7741
CrossRef
Pubmed
Google scholar
|
[17] |
Jiang Y, Marang L, Tamis J, van Loosdrecht M C M, Dijkman H, Kleerebezem R (2012). Waste to resource: Converting paper mill wastewater to bioplastic. Water Research, 46(17): 5517–5530
CrossRef
Pubmed
Google scholar
|
[18] |
Kang Z, Du L, Kang J, Wang Y, Wang Q, Liang Q, Qi Q (2011). Production of succinate and polyhydroxyalkanoate from substrate mixture by metabolically engineered Escherichia coli. Bioresource Technology, 102(11): 6600–6604
CrossRef
Pubmed
Google scholar
|
[19] |
Kumar M, Ghosh P, Khosla K, Thakur I S (2018). Recovery of polyhydroxyalkanoates from municipal secondary wastewater sludge. Bioresource Technology, 255: 111–115
CrossRef
Pubmed
Google scholar
|
[20] |
Laycock B, Arcos-Hernandez M V, Langford A, Pratt S, Werker A, Halley P J, Lant P A (2014). Crystallisation and fractionation of selected polyhydroxyalkanoates produced from mixed cultures. New Biotechnology, 31(4): 345–356
CrossRef
Pubmed
Google scholar
|
[21] |
Li R, Zhang H, Qi Q (2007). The production of polyhydroxyalkanoates in recombinant Escherichia coli. Bioresource Technology, 98(12): 2313–2320
CrossRef
Pubmed
Google scholar
|
[22] |
Li Y(2009). Study on isolation and fermentation condition of PHA producing bacteria base on acid-producing wastewater. Dissertation for the Master Degree. Harbin: Harbin Institute of Technology (in Chinese)
|
[23] |
Obruca S, Benesova P, Kucera D, Petrik S, Marova I (2014). Biotechnological conversion of spent coffee grounds into polyhydroxyalkanoates and carotenoids. New Biotechnology, 31: S39–S40
CrossRef
Google scholar
|
[24] |
Oliveira C S S, Silva C E, Carvalho G, Reis M A (2017). Strategies for efficiently selecting PHA producing mixed microbial cultures using complex feedstocks: Feast and famine regime and uncoupled carbon and nitrogen availabilities. New Biotechnology, 37(Pt A): 69–79
CrossRef
Pubmed
Google scholar
|
[25] |
Reddy C S K, Ghai R, Rashmi V C, Kalia
CrossRef
Pubmed
Google scholar
|
[26] |
Serafim L S, Lemos P C, Albuquerque M G E, Reis M A M (2008). Strategies for PHA production by mixed cultures and renewable waste materials. Applied Microbiology and Biotechnology, 81(4): 615–628
CrossRef
Pubmed
Google scholar
|
[27] |
Valentino F, Beccari M, Fraraccio S, Zanaroli G, Majone M (2014). Feed frequency in a sequencing batch reactor strongly affects the production of polyhydroxyalkanoates (PHAs) from volatile fatty acids. New Biotechnology, 31(4): 264–275
CrossRef
Pubmed
Google scholar
|
[28] |
Valentino F, Karabegovic L, Majone M, Morgan-Sagastume F, Werker A (2015). Polyhydroxyalkanoate (PHA) storage within a mixed-culture biomass with simultaneous growth as a function of accumulation substrate nitrogen and phosphorus levels. Water Research, 77: 49–63
CrossRef
Pubmed
Google scholar
|
[29] |
Valentino F, Morgan-Sagastume F, Campanari S, Villano M, Werker A, Majone M (2017). Carbon recovery from wastewater through bioconversion into biodegradable polymers. New Biotechnology, 37(Pt A): 9–23
CrossRef
Pubmed
Google scholar
|
[30] |
Wang Q (2012). Research on short chain fatty acids production by excess sludge hydrolysis and acidification. Dissertation for the Master Degree. Beijing: Tsinghua University (in Chinese)
|
[31] |
Wen Q, Chen Z, Tian T, Chen W (2010). Effects of phosphorus and nitrogen limitation on PHA production in activated sludge. Journal of Environmental Sciences-China, 22(10): 1602–1607
CrossRef
Pubmed
Google scholar
|
[32] |
Wen Q, Chen Z, Wang C, Ren N (2012). Bulking sludge for PHA production: Energy saving and comparative storage capacity with well-settled sludge. Journal of Environmental Sciences-China, 24(10): 1744–1752
CrossRef
Pubmed
Google scholar
|
/
〈 | 〉 |