Influence of hydraulic retention time on behavior of antibiotics and antibiotic resistance genes in aerobic granular reactor treating biogas slurry

Highlights

Longer HRT can enhance degradation rate of sulfamethoxazole in granular reactor.

Longer HRT can reduce accumulated concentrations of TCs and QNs in sludge.

Longer HRT may have increased relative abundances of ARGs in aerobic granules.

Abstract

The behavior of antibiotics and the corresponding resistance genes in aerobic granular reactors for treating biogas slurry under different hydraulic retention times (10.7 h, R1; 8 h, R2) was investigated in this study. The results indicated that the hydraulic retention time could affect the effluent concentrations and removal efficiencies of sulfonamides. The average removal rates of tetracyclines, fluoroquinolones, and sulfonamides were 63%, 46%, and 90% in R1, and 62%, 46%, and 86% in R2, respectively. Although the removal efficiencies of tetracyclines and fluoroquinolones were similar in both reactors, the respective accumulated concentrations of tetracyclines and fluoroquinolones in R1 were 7.00 and 11.15 µg/g SS, which were lower than those in R2 (8.92 and 13.37 µg/g SS, respectively). The difference in the relative abundance of target antibiotic resistance genes between both reactors was not significant, yet the average relative abundances of all target resistance genes in R1 were higher than those in R2 after 45 days of operation. The results of this study suggested that a longer hydraulic retention time could enhance the antibiotic removal ability of aerobic granular sludge, yet it may also increase the risk of surplus sludge utilization from a resistance genes point of view.

Graphical abstract

Keywords

Aerobic granules / Swine wastewater / Tetracyclines / Fluoroquinolones / Sulfonamides

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Acknowledgements

This work was supported by Science and Technology Project of Fujian Province (No. 2018Y0083); Research fund of Key Laboratory of Environmental Biotechnology, Fujian Province University (No. EBL2018008); National Natural Science Foundation of China (Grant No. 51878582); and STS Project of Fujian-CAS (No. 2016T3006).

Supplementary material

is available in the online version of this article at https://doi.org/10.1007/s11783-019-1115-6 and is accessible for authorized users.

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