Response of indigenous Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based on the sensing of fluorescence probes
Xia Hou, Liping Huang, Peng Zhou, Hua Xue, Ning Li
Response of indigenous Cd-tolerant electrochemically active bacteria in MECs toward exotic Cr(VI) based on the sensing of fluorescence probes
Cell membrane of indigenous Cd-tolerant EAB harbored more cadmium than chromium.
Indigenous Cd-tolerant EAB cytoplasm located more chromium than cadmium.
Simultaneously quantitatively imaging Cd(II) and Cr(III) ions in EAB was achieved.
Current accelerated the harboring of cadmium in EAB at an initial 2 h.
Current directed the accumulation of more chromium in EAB over time.
Electrochemically active bacteria (EAB) on the cathodes of microbial electrolysis cells (MECs) can remove metals from the catholyte, but the response of these indigenous EAB toward exotic metals has not been examined, particularly from the perspective of the co-presence of Cd(II) and Cr(VI) in a wastewater. Four known indigenous Cd-tolerant EAB of Ochrobactrum sp X1, Pseudomonas sp X3, Pseudomonas delhiensis X5, and Ochrobactrum anthropi X7 removed more Cd(II) and less Cr(VI) in the simultaneous presence of Cd(II) and Cr(VI), compared to the controls with individual Cd(II) or single Cr(VI). Response of these EAB toward exotic Cr(VI) was related to the associated subcellular metal distribution based on the sensing of fluorescence probes. EAB cell membrane harbored more cadmium than chromium and cytoplasm located more chromium than cadmium, among which the imaging of intracelluler Cr(III) ions increased over time, contrary to the decreased trend for Cd(II) ions. Compared to the controls with single Cd(II), exotic Cr(VI) decreased the imaging of Cd(II) ions in the EAB at an initial 2 h and negligibly affected thereafter. However, Cd(II) diminished the imaging of Cr(III) ions in the EAB over time, compared to the controls with individual Cr(VI). Current accelerated the harboring of cadmium at an initial 2 h and directed the accumulation of chromium in EAB over time. This study provides a viable approach for simultaneously quantitatively imaging Cd(II) and Cr(III) ions in EAB and thus gives valuable insights into the response of indigenous Cd-tolerant EAB toward exotic Cr(VI) in MECs.
Microbial electrolysis cell / Electrochemically active bacteria / Cd-tolerant bacteria / Cd(II) and Cr(VI) / Fluorescence probe
[1] |
American Public Health Association (1998). In: American Water Works Association, Water Pollution Control Federation, eds. Standard methods for the examination of water and wastewater. 20th ed. Washington, DC
|
[2] |
Antonioli P, Lampis S, Chesini I, Vallini G, Rinalducci S, Zolla L, Righetti P G (2007). Stenotrophomonas maltophilia SeITE02, a new bacterial strain suitable for bioremediation of selenite-contaminated environmental matrices. Applied and Environmental Microbiology, 73(21): 6854–6863
CrossRef
Pubmed
Google scholar
|
[3] |
Banerjee I, Burrell B, Reed C, West A C, Banta S (2017). Metals and minerals as a biotechnology feedstock: Engineering biomining microbiology for bioenergy applications. Current Opinion in Biotechnology, 45: 144–155
CrossRef
Pubmed
Google scholar
|
[4] |
Cao L, Sun W, Zhang Y, Feng S, Dong J, Zhang Y, Rittmann B E (2017). Competition for electrons between reductive dechlorination and denitrification. Frontiers of Environmental Science & Engineering, 11(6): 14
CrossRef
Google scholar
|
[5] |
Chen Y, Shen J, Huang L, Pan Y, Quan X (2016). Enhanced Cd(II) removal with simultaneous hydrogen production in biocathode microbial electrolysis cells in the presence of acetate or NaHCO3. International Journal of Hydrogen Energy, 41(31): 13368–13379
CrossRef
Google scholar
|
[6] |
Chien C C, Jiang M H, Tsai M R, Chien C C (2011). Isolation and characterization of an environmental cadmium- and tellurite-resistant Pseudomonas strain. Environmental Toxicology and Chemistry, 30(10): 2202–2207
CrossRef
Pubmed
Google scholar
|
[7] |
Chien C C, Lin B C, Wu C H (2013). Biofilm formation and heavy metal resistance by an environmental Pseudomonas sp. Biochemical Engineering Journal, 78(SI): 132‒137
|
[8] |
Deb S, Ahmed S F, Basu M (2013). Metal accumulation in cell wall: A possible mechanism of cadmium resistance by Pseudomonas stutzeri. Bulletin of Environmental Contamination and Toxicology, 90(3): 323–328
CrossRef
Pubmed
Google scholar
|
[9] |
Grąz M, Pawlikowska-Pawlęga B, Jarosz-Wilkołazka A (2015). Intracellular distribution of cadmium during the growth of Abortiporus biennis on cadmium-amended media. Canadian Journal of Microbiology, 61(8): 545–554
CrossRef
Pubmed
Google scholar
|
[10] |
Hasany M, Mahdi Mardanpour M, Yaghmaei S (2016). Biocatalysts in microbial electrolysis cells: A review. International Journal of Hydrogen Energy, 41(3): 1477–1493
CrossRef
Google scholar
|
[11] |
Huang L, Gan L, Wang N, Quan X, Logan B E, Chen G (2012). Mineralization of pentachlorophenol with enhanced degradation and power generation from air cathode microbial fuel cells. Biotechnology and Bioengineering, 109(9): 2211–2221
CrossRef
Pubmed
Google scholar
|
[12] |
Huang L, Jiang L, Wang Q, Quan X, Yang J, Chen L (2014). Cobalt recovery with simultaneous methane and acetate production in biocathode microbial electrolysis cells. Chemical Engineering Journal, 253: 281–290
CrossRef
Google scholar
|
[13] |
Huang L, Wang Q, Jiang L, Zhou P, Quan X, Logan B E (2015). Adaptively evolving bacterial communities for complete and selective reduction of Cr(VI), Cu(II)and Cd(II) in biocathode bioelectrochemical systems. Environmental Science & Technology, 49(16): 9914–9924 doi:10.1021/acs.est.5b00191
Pubmed
|
[14] |
Huang L, Wang Q, Quan X, Liu Y, Chen G (2013). Bioanodes/biocathodes formed at optimal potentials enhance subsequent pentachlorophenol degradation and power generation from microbial fuel cells. Bioelectrochemistry (Amsterdam, Netherlands), 94: 13–22
CrossRef
Pubmed
Google scholar
|
[15] |
Huang L, Xue H, Zhou Q, Zhou P, Quan X (2018). Imaging and distribution of Cd(II) ions in electrotrophs and its response to current and electron transfer inhibitor in microbial electrolysis cells. Sensors and Actuators. B, Chemical, 255: 244–254
CrossRef
Google scholar
|
[16] |
Iskander S M, Brazil B, Novak J T, He Z (2016). Resource recovery from landfill leachate using bioelectrochemical systems: Opportunities, challenges, and perspectives. Bioresource Technology, 201: 347–354
CrossRef
Pubmed
Google scholar
|
[17] |
Jang A, Seo Y, Bishop P L (2005). The removal of heavy metals in urban runoff by sorption on mulch. Environmental Pollution, 133(1): 117–127
CrossRef
Pubmed
Google scholar
|
[18] |
Kong F, Liang B, Yun H, Cheng H, Wang A, Ren N (2015). Cathodic degradation of antibiotics: Characterization and pathway analysis. Water Research,72(SI): 281‒292.
|
[19] |
Kotaś J, Stasicka Z (2000). Chromium occurrence in the environment and methods of its speciation. Environmental Pollution, 107(3): 263–283
CrossRef
Pubmed
Google scholar
|
[20] |
Li W W, Sheng G P, Liu X W, Yu H Q (2011). Recent advances in the separators for microbial fuel cells. Bioresource Technology, 102(1): 244–252
CrossRef
Pubmed
Google scholar
|
[21] |
Lutfor Rahman M, Sarkar S M, Mohd Yusoff M (2016). Efficient removal of heavy metals from electroplating wastewater using polymer ligands. Frontiers of Environmental Science & Engineering, 10(2): 352–361 doi:10.1007/s11783-015-0783-0
|
[22] |
Mao J, Wang L, Dou W, Tang X, Yan Y, Liu W (2007). Tuning the selectivity of two chemosensors to Fe(III) and Cr(III). Organic Letters, 9(22): 4567–4570
CrossRef
Pubmed
Google scholar
|
[23] |
Miretzky P, Saralegui A, Fernandez C A (2004). Aquatic macrophytes potential for simultaneous heavy metal removal. Chemosphere, 57(8): 997–1005
CrossRef
Pubmed
Google scholar
|
[24] |
Mukherjee P, Roy P (2016). Genomic potential of Stenotrophomonas maltophilia in bioremediation with an assessment of its multifaceted role in our environment. Frontiers in Microbiology, 7: 967
CrossRef
Pubmed
Google scholar
|
[25] |
Nancharaiah Y V, Mohan S V, Lens P N L (2016). Biological and bioelectrochemical recovery of critical and scarce metals. Trends in Biotechnology, 34(2): 137–155
CrossRef
Pubmed
Google scholar
|
[26] |
Osborn M J, Munson R (1974). Separation of the inner (cytoplasmic) and outer membranes of Gram-negative bacteria. Methods in Enzymology, 31: 642–653
CrossRef
Pubmed
Google scholar
|
[27] |
Pal R, Tewari S, Rai J P N (2009). Metals sorption from aqueous solutions by Kluyveromyces marxianus: Process optimization, equilibrium modeling and chemical characterization. Biotechnology Journal, 4(10): 1471–1478
CrossRef
Pubmed
Google scholar
|
[28] |
Qian Y, Huang L, Pan Y, Quan X, Lian H, Yang J (2018). Dependency of migration and reduction of mixed Cr2O72‒, Cu2+ and Cd2+ on electric field, ion exchange membrane and metal concentration in microbial fuel cells. Separation and Purification Technology, 192: 78–87
CrossRef
Google scholar
|
[29] |
Ren S T, Li M C, Sun J Y, Bian Y H, Zuo K C, Zhang X Y, Liang P, Huang X (2017). A novel electrochemical reactor for nitrogen and phosphorus recovery from domestic wastewater. Frontiers of Environmental Science & Engineering, 11(4): 17
CrossRef
Google scholar
|
[30] |
Rossbach S, Kukuk M L, Wilson T L, Feng S F, Pearson M M, Fisher M A (2000). Cadmium-regulated gene fusions in Pseudomonas fluorescens. Environmental Microbiology, 2(4): 373–382
CrossRef
Pubmed
Google scholar
|
[31] |
Saraswat S, Rai J P N (2010). Heavy metal adsorption from aqueous solution using Eichhornia crassipes dead biomass. International Journal of Mineral Processing, 94(3‒4): 203–206
CrossRef
Google scholar
|
[32] |
Shen J, Huang L, Zhou P, Quan X, Puma G L (2017). Correlation between circuital current, Cu(II) reduction and cellular electron transfer in EAB isolated from Cu(II)-reduced biocathodes of microbial fuel cells. Bioelectrochemistry (Amsterdam, Netherlands), 114: 1–7
CrossRef
Pubmed
Google scholar
|
[33] |
Siunova T V, Siunov A V, Kochetkov V V, Boronin A M (2009). The cnr-like operon in strain Comamonas sp. encoding resistance to cobalt and nickel. Russian Journal of Genetics, 45(3): 336–341
CrossRef
Pubmed
Google scholar
|
[34] |
Stephen J R, Chang Y J, Macnaughton S J, Kowalchuk G A, Leung K T, Flemming C A, White D C (1999). Effect of toxic metals on indigenous soil b-subgroup proteobacterium ammonia oxidizer community structure and protection against toxicity by inoculated metal-resistant bacteria. Applied and Environmental Microbiology, 65(1): 95–101
Pubmed
|
[35] |
Tao Y, Xue H, Huang L, Zhou P, Yang W, Quan X, Yuan J (2017). Fluorescent probe based subcellular distribution of Cu(II) ions in living electrotrophs isolated from Cu(II)-reduced biocathodes of microbial fuel cells. Bioresource Technology, 225: 316–325
CrossRef
Pubmed
Google scholar
|
[36] |
Venkata Mohan S, Velvizhi G, Annie Modestra J, Srikanth S (2014). Microbial fuel cell: Critical factors regulating bio-catalyzed electrochemical process and recent advancements. Renewable & Sustainable Energy Reviews, 40: 779–797
CrossRef
Google scholar
|
[37] |
Wang A, Cheng H, Ren N, Cui D, Lin N, Wu W (2012). Sediment microbial fuel cell with floating biocathode for organic removal and energy recovery. Frontiers of Environmental Science & Engineering, 6(4): 569–574
CrossRef
Google scholar
|
[38] |
Wang H, Ren Z J (2014). Bioelectrochemical metal recovery from wastewater: A review. Water Research, 66: 219–232
CrossRef
Pubmed
Google scholar
|
[39] |
Wang M, Tan Q, Chiang J F, Li J (2017a). Recovery of rare and precious metals from urban mines: A review. Frontiers of Environmental Science & Engineering, 11(5): 1
CrossRef
Google scholar
|
[40] |
Wang Q, Huang L, Pan Y, Zhou P, Quan X, Logan B E, Chen H (2016). Cooperative cathode electrode and in situ deposited copper for subsequent enhanced Cd(II) removal and hydrogen evolution in bioelectrochemical systems. Bioresource Technology, 200: 565–571
CrossRef
Pubmed
Google scholar
|
[41] |
Wang Q, Huang L, Quan X, Zhao Q (2017b). Preferable utilization of in-situ produced H2O2 rather than externally added for efficient deposition of tungsten and molybdenum in microbial fuel cells. Electrochimica Acta, 247: 880–890
CrossRef
Google scholar
|
[42] |
Xu L, He M L, Yang H B, Qian X (2013). A simple fluorescent probe for Cd2+ in aqueous solution with high selectivity and sensitivity. Dalton Transactions (Cambridge, England), 42(23): 8218–8222
CrossRef
Pubmed
Google scholar
|
[43] |
Xue H, Zhou P, Huang L, Quan X, Yuan J (2017). Cathodic Cr(VI) reduction by electrochemically active bacteria sensed by fluorescent probe. Sensors and Actuators. B, Chemical, 243: 303–310
CrossRef
Google scholar
|
[44] |
Yang Y, Zhao Q, Feng W, Li F (2013). Luminescent chemodosimeters for bioimaging. Chemical Reviews, 113(1): 192–270
CrossRef
Pubmed
Google scholar
|
[45] |
Yu Q, Fein J B (2017). Enhanced removal of dissolved Hg(II), Cd(II), and Au(III) from water by Bacillus subtilis bacterial biomass containing an elevated concentration of sulfhydryl sites. Environmental Science & Technology, 51(24): 14360–14367
CrossRef
Pubmed
Google scholar
|
[46] |
Zaki S, Farag S (2010). Isolation and molecular characterization of some copper biosorped strains. International Journal of Environmental Science and Technology, 7(3): 553–560
CrossRef
Google scholar
|
[47] |
Zhang F, Yu S, Li J, Li W, Yu H (2016). Mechanisms behind the accelerated extracellular electron transfer in Geobacter sulfurreducens DL-1 by modifying gold electrode with self-assembled monolayers. Frontiers of Environmental Science & Engineering, 10(3): 531–538
CrossRef
Pubmed
Google scholar
|
[48] |
Zhao Q, Yu H, Zhang W, Tetteh Kabutey F, Jiang J, Zhang Y, Wang K, Ding J (2017). Microbial fuel cell with high content solid wastes as substrates: A review. Frontiers of Environmental Science & Engineering, 11(2): 13
CrossRef
Google scholar
|
/
〈 | 〉 |