Vacuum promotes metabolic shifts and increases biogenic hydrogen production in dark fermentation systems

Abstract

The successful operation of any type of hydrogen-producing bioreactor depends on the performance of the microorganisms present in the system. Both substrate and partial gas pressures are crucial factors affecting dark fermentation metabolic pathways. The main objective of this study was to evaluate the impact of both factors on hydrogen production using anaerobic granular sludge as inoculum and, secondly, to study the metabolic shifts of an anaerobic community subjected to low partial gas pressures. With this goal in mind, seven different wastewater (four synthetic media, two industrial wastewater, and one domestic effluent) and the effect of applying vacuum on the systems were analyzed. The application of vacuum promoted an increase in the diversity of hydrogen-producing bacteria, such as Clostridium, and promoted the dominance of acetoclastic- over hydrogenotrophic methanogens. The application of different media promoted a wide variety of metabolic pathways. Nevertheless, reduction of the hydrogen partial pressure by application of vacuum lead to further oxidation of reaction intermediates irrespective of the medium used, which resulted in higher hydrogen and methane production, and improved the COD removal. Interestingly, vacuum greatly promoted biogenic hydrogen production from a real wastewater, which opens possibilities for future application of dark fermentation systems to enhance biohydrogen yields.

Keywords

dark fermentation / biohydrogen / wastewaters / vacuum

References

[1] Stronach S M, Rudd T, Lester J N. Anaerobic Digestion Processes in Industrial Wastewater Treatment. Biotechnology Monographs. Berlin: Germany Springer-Verlag, 1986

[2] Hu B, Chen S. Pretreatment of methanogenic granules for immobilized hydrogen fermentation. International Journal of Hydrogen Energy, 2007, 32(15): 3266–3273

[3] Mizuno O, Dinsdale R, Hawkes F R, Hawkes D L, Noile T. Enhancement of hydrogen production by nitrogen gas sparging. Bioresource Technology, 2000, 73(1): 59–65

[4] Karlsson A, Vallin L, Ejelertsson J. Effects of temperature hydraulic retention time and hydrogen extraction rate on hydrogen production from the fermentation food industry residues and manure. International Journal of Hydrogen Energy, 2008, 33(3): 953–962

[5] Liang T M, Cheng S S, Wu K L. Behavioral study on hydrogen fermentation reactor installed with silicone rubber membrane. International Journal of Hydrogen Energy, 2002, 27(11–12): 1157–1165

[6] Clark I C, Zhang R H, Upadhyaya S K. The effect of low pressure and mixing on biological hydrogen production via anaerobic fermentation. International Journal of Hydrogen Energy, 2012, 37(15): 11504–11513

[7] Lee K S, Tseng T S, Liu Y W, Hsiao Y D. Enhancing the performance of dark fermentative hydrogen production using a reduced pressure fermentation strategy. International Journal of Hydrogen Energy, 2012, 37(20): 15556–15562

[8] Show K Y, Lee D J, Chang J S. Bioreactor and process design for biohydrogen production. Bioresource Technology, 2011, 102(18): 8524–8533

[9] Tang G L, Huang J, Sun Z J, Tang Q Q, Yan C H, Liu G Q. Biohydrogen production from cattle wastewater by enriched anaerobic mixed consortia: influence of fermentation temperature and pH. Journal of Bioscience and Bioengineering, 2008, 106(1): 80–87

[10] Fang H H P, Liu H, Zhang T. Characterization of a hydrogen-producing granular sludge. Biotechnology and Bioengineering, 2002, 78(1): 44–52

[11] Lee K S, Wu J F, Lo Y S, Lo Y C, Lin P J, Chang J S. Anaerobic hydrogen production with an efficient carrier-induced granular sludge bed bioreactor. Biotechnology and Bioengineering, 2004, 87(5): 648–657

[12] Hawkes F R, Hussy I, Kyazze G, Dinsdale R, Hawkes D L. Continuous dark fermentative hydrogen production by mesophilic microflora: Principles and progress. International Journal of Hydrogen Energy, 2007, 32(2): 172–184

[13] Niu K, Zhang X, Tan W S, Zhu M L. Characterization of fermentative hydrogen production with Klebsiella pneumoniae ECU-15 isolated from anaerobic sewage sludge. International Journal of Hydrogen Energy, 2010, 35(1): 71–80

[14] Garcia Mancha N, Puyol D, Monsalvo V M, Rajhi H, Mohedano A F, Rodriguez J J. Anaerobic treatment of wastewater from used industrial oil recovery. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 2012, 87(9): 1320–1328

[15] Sanz J L, Rodríguez N, Amils R. The action of antibiotics on the anaerobic digestion process. Applied Microbiology and Biotechnology, 1996, 46(5–6): 587–592

[16] Standard Methods for the Examination of Water and Wastewater. Washington DC: American Public Health Association/American Water Works Association/Water Environment Federation, 1998

[17] Fang H H P, Zhang T, Liu H. Microbial diversity of a mesophilic hydrogen-producing sludge. Applied Microbiology and Biotechnology, 2002, 58(1): 112–118

[18] Lo Y C, Chen W M, Hung C H, Chen S D, Chang J S. Dark H₂ fermentation from sucrose and xylose using H₂-producing indigenous bacteria: feasibility and kinetic studies. Water Research, 2008, 42(4–5): 827–842

[19] Kanso S, Dasri K, Tingthong S, Watanapokasin R Y. Diversity of cultivable hydrogen-producing bacteria isolated from agricultural soils, waste water sludge and cow dung. International Journal of Hydrogen Energy, 2011, 36(14): 8735–8742

[20] Toh H, Sharma V K, Oshima K, Kondo S, Hattori M, Ward F B, Free A, Taylor T D. Complete genome sequences of Arcobacter butzleri ED-1 and Arcobacter sp. strain L, both isolated from a microbial fuel cell. Journal of Bacteriology, 2011, 193(22): 6411–6412

[21] Liu W T, Chan O C, Fang H H P. Characterization of microbial community in granular sludge treating brewery wastewater. Water Research, 2002, 36(7): 1767–1775

[22] Liu F, Fang B. Optimization of bio-hydrogen production from biodiesel wastes by Klebsiella pneumoniae. Biotechnology Journal, 2007, 2(3): 374–380

[23] Sun W, Sierra-Alvarez R, Milner L, Field J A. Anaerobic oxidation of arsenite linked to chlorate reduction. Applied and Environmental Microbiology, 2010, 76(20): 6804–6811

[24] Diaz E E, Stams F, Amils R, Sanz J L. Phenotypic properties and microbial diversity of methanogenic granules from a full-scale UASB reactor treating brewery wastewaters. Applied and Environmental Microbiology, 2006, 72(7): 4942–4949

[25] Jun Y S, Yu S H, Ryu K G, Lee T J. Kinetic study of pH effects on biological hydrogen production by a mixed culture. Journal of Microbiology and Biotechnology, 2008, 18(6): 1130–1135

[26] Nicolau J M, Guwy A, Dinsdale R, Premier G, Esteves S. Production of hydrogen from sewage biosolids in a continuously fed bioreactor: Effect of hydraulic retention time and sparging. International Journal of Hydrogen Energy, 2010, 35(2): 469–478

[27] Mead G C. The amino acid-fermenting clostridia. Journal of General Microbiology, 1971, 67(1): 47–56

[28] Staples C A, Williams J B, Craig G R, Roberts K M. Fate, effects and potential environmental risks of ethylene glycol: a review. Chemosphere, 2001, 43(3): 377–383

[29] Antonopoulou G, Gaval N, Skiadas I V, Lyberatos G. Influence of pH fermentative hydrogen production from sweet sorghum extract. International Journal of Hydrogen Energy, 2010, 35(5): 1921–1928

[30] Lay J J, Fan K S, Chang J L, Ku C H. Influence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock sludge. International Journal of Hydrogen Energy, 2003, 28(12): 1361–1367

[31] Hussy I, Hawkes F R, Dinsdale R, Hawkes D L. Continuous fermentative hydrogen production from a wheat starch co-product by mixed microflora. Biotechnology and Bioengineering, 2003, 84(6): 619–626

[32] Kalia V C, Purohit H J. Microbial diversity and genomics in aid of bioenergy. Journal of Industrial Microbiology & Biotechnology, 2008, 35(5): 403–419

[33] Speece R E. Anaerobic Biotechnology (for Industrial wastewater). Nashville, Tennessee: Archae Press, 1996

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