Ecotoxicity assessment of soil irrigated with domestic wastewater using different extractions

Abstract

The toxicity of soil irrigated with treated domestic wastewater (site A) and untreated gray wastewater (site B) were investigated. Soil extracts were prepared using distilled water, acid solvent (0.1 mol·L^-1 HCl), and organic solvent (acetone:petroleum ether:cyclohexane= 1:1:1) to understand the type of pollutants responsible for the ecotoxicity associated with wastewater irrigation. The soil toxicity was assessed using a luminescence inhibition assay with Vibrio fischeri for acute toxicity, a micronucleus assay with Vicia faba root tips and a single cell gel electrophoresis assay of mice lymphocytes for genotoxicity. The physicochemical properties and the heavy metal (HM) contents of the irrigated soil were also analyzed. The results indicated that the wastewater irrigation at site A had no effects on the soil properties. With the exception of Pb, Zn, Fe, and Mn, the accumulation of HMs (Cu, Ni, and Cr) occurred. However, the irrigation at site A did not result in obvious acute toxicity or genotoxicity in the soil. The soil properties changed greatly, and HMs (Cu, Ni, and Cr) accumulated in site B. There were significant increases in the acute toxic and genotoxic effects in the soils from site B. The ecotoxicity in site B came primarily from organic-extractable pollutants.

Keywords

ecotoxicology / domestic wastewater / soil irrigation / risk assessment / organic extraction

References

[1] Castro E, Mañas M P, Heras J D L. Effects of wastewater irrigation on soil properties and turfgrass growth. Water Science & Technology, 2011, 63(8): 1678–1688

[2] Abdu N, Abdulkadir A, Agbenin J O, Buerkert A. Vertical distribution of heavy metals in wastewater irrigated vegetable garden soils of three West African cities. Nutrient Cycling in Agroecosystems, 2011, 89(3): 387–397

[3] Gibson R, Durán-Álvarez J C, Estrada K L, Chávez A, Jiménez Cisneros B. Accumulation and leaching potential of some pharmaceuticals and potential endocrine disruptors in soils irrigated with wastewater in the Tula Valley, Mexico. Chemosphere, 2010, 81(11): 1437–1445

[4] Zeng L, Wang T, Han W, Yuan B, Liu Q, Wang Y, Jiang G. Spatial and vertical distribution of short chain chlorinated paraffins in soils from wastewater irrigated farmlands. Environmental Science & Technology, 2011, 45(6): 2100–2106

[5] Tarchouna L G, Merdy P, Raynaud M, Pfeifer H R, Lucas Y. Effects of long-term irrigation with treated wastewater. Part I: Evolution of soil physico-chemical properties. Applied Geochemistry, 2010, 25(11): 1703–1710

[6] Aleem A, Malik A. Genotoxic hazards of long-term application of wastewater on agricultural soil. Mutation Research, 2003, 538(1–2): 145–154

[7] Song Y F, Gong P, Wilke B M, Zhang W, Song X Y, Sun T H, Ackland M L. Genotoxicity assessment of soils from wastewater irrigation areas and bioremediation sites using the Vicia faba root tip micronucleus assay. Journal of Environmental Monitoring, 2007, 9(2): 182–186

[8] Yu G, Xiao R, Wang D, Zhou J, Wang Z. Assessing the ecological risk of soil irrigated with wastewater using in vitro cell bioassays. Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances & Environmental Engineering, 2008, 43(14): 1618–1627

[9] Song Y F, Wilke B M, Song X Y, Gong P, Zhou Q X, Yang G F. Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals (HMs) as well as their genotoxicity in soil after long-term wastewater irrigation. Chemosphere, 2006, 65(10): 1859–1868

[10] Qiao M, Chen Y, Wang C X, Wang Z, Zhu Y G. DNA damage and repair process in earthworm after in-vivo and in vitro exposure to soils irrigated by wastewaters. Environmental Pollution, 2007, 148(1): 141–147

[11] Courchesne F, Kruyts N, Legrand P. Labile zinc concentration and free copper ion activity in the rhizosphere of forest soils. Environmental Toxicology and Chemistry, 2006, 25(3): 635–642

[12] Lagomarsino A, Mench M, Marabottini R, Pignataro A, Grego S, Renella G, Stazi S R. Copper distribution and hydrolase activities in a contaminated soil amended with dolomitic limestone and compost. Ecotoxicology and Environmental Safety, 2011, 74(7): 2013–2019

[13] Cabrera G L, Rodriguez D M G. Genotoxicity of soil from farmland irrigated with wastewater using three plant bioassays. Mutation Research, 1999, 426(2): 211–214

[14] Ehrlichmann H, Dott W, Eisentraeger A. Assessment of the water-extractable genotoxic potential of soil samples from contaminated sites. Ecotoxicology and Environmental Safety, 2000, 46(1): 73–80

[15] China Environmental Protection Bureau. Standard Methods for Examination of Water and Wastewater. 4th ed. Beijing: Chinese Environmental Science Press, 2004 (in Chinese)

[16] Rusjan D, Strlič M, Pucko D, Korošec-Koruza Z. Copper accumulation regarding the soil characteristics in Sub-Mediterranean vine yards of Slovenia. Geoderma, 2007, 141(1–2): 111–118

[17] Liang W, Chen L, Sui L, Yu J, Wang L, Shi H. Assessment of detoxification of microcystin extracts using electrochemical oxidation. Journal of Environmental Science and Health. Part A, Toxic/Hazardous Substances & Environmental Engineering, 2011, 46(10): 1102–1112

[18] Majer B J, Grummt T, Uhl M, Knasmüller S. Use of plant bioassays for the detection of genotoxins in the aquatic environment. Acta Hydrochimica et Hydrobiologica, 2005, 33(1): 45–55

[19] Marcato-Romain C E, Guiresse M, Cecchi M, Cotelle S, Pinelli E. New direct contact approach to evaluate soil genotoxicity using the Vicia faba micronucleus test. Chemosphere, 2009, 77(3): 345–350

[20] Końca K, Lankoff A, Banasik A, Lisowska H, Kuszewski T, Góźdź S, Koza Z, Wojcik A. A cross-platform public domain PC image-analysis program for the comet assay. Mutation Research, 2003, 534(1–2): 15–20

[21] Xu J, Wu L, Chang A C, Zhang Y. Impact of long-term reclaimed wastewater irrigation on agricultural soils: a preliminary assessment. Journal of Hazardous Materials, 2010, 183(1–3): 780–786

[22] Yao H, Zhang S, Xue X, Yang J, Hu K, Yu X. Influence of the sewage irrigation on the agricultural soil properties in Tongliao City, China. Frontiers of Environmental Science & Engineering, 2013, 7(2): 273–280

[23] Singh A, Sharma R K, Agrawal M, Marshall F M. Risk assessment of heavy metal toxicity through contaminated vegetables from waste water irrigated area of Varanasi, India. Tropical Ecology, 2010, 51(2S): 375–387

[24] Duan R, Fedler C B, Sheppard C D. Field study of salt balance of a land application system. Water, Air, & Soil Pollution, 2011, 215(1–4): 43–54

[25] Fonseca A, Herpin U, Paula A M, Victória R L, Melfi A J. Agricultural use of treated sewage effluents: agronomic and environmental implications and perspectives for Brazil. Scientia Agricola, 2007, 64(2): 194–209

[26] Girotti S, Ferri E N, Fumo M G, Maiolini E. Monitoring of environmental pollutants by bioluminescent bacteria. Analytica Chimica Acta, 2008, 608(1): 2–29

[27] Acheson C M, Zhou Q, Shan Y, Sayles G D, Kupferle M J. Comparing the solid phase and saline extract Microtox assays for two polycyclic aromatic hydrocarbon-contaminated soils. Environmental Toxicology and Chemistry, 2004, 23(2): 245–251

[28] Shen K, Shen C, Lu Y, Tang X, Zhang C, Chen X, Shi J, Lin Q, Chen Y. Hormesis response of marine and freshwater luminescent bacteria to metal exposure. Biological Research, 2009, 42(2): 183–187

[29] Wang L J, Liu S S, Yuan J, Liu H L. Remarkable hormesis induced by 1-ethyl-3-methyl imidazolium tetrafluoroborate on Vibrio qinghaiensis sp.-Q67. Chemosphere, 2011, 84(10): 1440–1445

[30] Frische T, Höper H. Soil microbial parameters and luminescent bacteria assays as indicators for in situ bioremediation of TNT-contaminated soils. Chemosphere, 2003, 50(3): 415–427

[31] Niemi R M, Heiskanen I, Ahtiainen J H, Rahkonen A, Mäntykoski K, Welling L, Laitinen P, Ruuttunen P. Microbial toxicity and impacts on soil enzyme activities of pesticides used in potato cultivation. Applied Soil Ecology, 2009, 41(3): 293–304

[32] Tang J, Wang M, Wang F, Sun Q, Zhou Q. Eco-toxicity of petroleum hydrocarbon contaminated soil. Journal of Environmental Sciences-China, 2011, 23(5): 845–851

[33] Chen Y, Wang C, Wang Z, Huang S. Assessment of the contamination and genotoxicity of soil irrigated with wastewater. Plant and Soil, 2004, 261(1–2): 189–196

[34] Lin D, Zhou Q, Xie X, Liu Y. Potential biochemical and genetic toxicity of triclosan as an emerging pollutant on earthworms (Eisenia fetida). Chemosphere, 2010, 81(10): 1328–1333

[35] Zhu J, Zhao Z Y, Lu Y T. Evaluation of genotoxicity of combined soil pollution by cadmium and phenanthrene on earthworm. Journal of Environmental Sciences-China, 2006, 18(6): 1210–1215

Acknowledgements

This study was supported by grants from the National Water Pollution Control and Management Technology Major Projects of China (2008ZX07314-006 and 2012ZX07307-001-006).

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg