Determination and occurrence of endocrine disrupting compounds, pharmaceuticals and personal care products in fish (Morone saxatilis)

Abstract

Endocrine disrupting compounds (EDCs), pharmaceuticals and personal care products (PPCPs) have attracted much attention due to widespread contamination in aquatic environment. In this study, we determined 13 EDCs and PPCPs in fish blood, bile and muscle by using gas chromatography-mass spectrometry (GC-MS). The limits of quantitation (LOQ) were in the ranges of 0.23–2.54, 0.22–2.36 ng·mL⁻¹, and 0.24–2.57 ng·g⁻¹ dry weight (dw) for fish blood, bile and muscle, respectively. Recoveries of target compounds spiked into sample matrices and passed through the entire analytical procedure ranged from 65% to 95%, from 60% to 92% and from 62% to 91% for blood, bile and muscle, respectively. The methods were applied to the analysis of fish from a lake in California. Target compounds were relatively low in bile, and only bisphenol A (BPA) and diclofenac were measurable near the LOQ. Seven of 13 compounds were detected in blood, with total concentrations up to 39 ng·mL⁻¹. Only BPA was frequently found in muscle, with mean concentration of 7.26 ng·g⁻¹ dw. The estimated daily intake of BPA through fish consumption for U.S. resident was significantly lower than the tolerable daily intake recommended by the European Food Safety Authority. This study showed that the exposure to the bisphenol A from fish diet is unlikely to pose a health risk.

Graphical abstract

Keywords

endocrine disrupting compounds (EDCs) / pharmaceuticals and personal care products (PPCPs) / fish / bisphenol A (BPA) / risk assessment

References

[1] Boxall A B A, Rudd M A, Brooks B W, Caldwell D J, Choi K, Hickmann S, Innes E, Ostapyk K, Staveley J P, Verslycke T, Ankley G T, Beazley K F, Belanger S E, Berninger J P, Carriquiriborde P, Coors A, Deleo P C, Dyer S D, Ericson J F, Gagné F, Giesy J P, Gouin T, Hallstrom L, Karlsson M V, Larsson D G, Lazorchak J M, Mastrocco F, McLaughlin A, McMaster M E, Meyerhoff R D, Moore R, Parrott J L, Snape J R, Murray-Smith R, Servos M R, Sibley P K, Straub J O, Szabo N D, Topp E, Tetreault G R, Trudeau V L, Van Der Kraak G. Pharmaceuticals and personal care products in the environment: what are the big questions? Environmental Health Perspectives, 2012, 120(9): 1221–1229

[2] Ratola N, Cincinelli A, Alves A, Katsoyiannis A, Occurrence of organic microcontaminants in the wastewater treatment process. A mini review. Journal of Hazardous Materials, 2012, 239–249: 1–18

[3] Gracia-Lor E, Sancho J V, Serrano R, Hernández F. Occurrence and removal of pharmaceuticals in wastewater treatment plants at the Spanish Mediterranean area of Valencia. Chemosphere, 2012, 87(5): 453–462

[4] Al Aukidy M, Verlicchi P, Jelic A, Petrovic M, Barcelò D. Monitoring release of pharmaceutical compounds: occurrence and environmental risk assessment of two WWTP effluents and their receiving bodies in the Po Valley, Italy. Science of the Total Environment, 2012, 438: 15–25

[5] Verlicchi P, Al Aukidy M, Zambello E. Occurrence of pharmaceutical compounds in urban wastewater: removal, mass load and environmental risk after a secondary treatment—a review. Science of the Total Environment, 2012, 429: 123–155

[6] Yu Y, Wu L, Chang A C. Seasonal variation of endocrine disrupting compounds, pharmaceuticals and personal care products in wastewater treatment plants. Science of the Total Environment, 2013, 442: 310–316

[7] Gibson R, Smith M D, Spary C J, Tyler C R, Hill E M. Mixtures of estrogenic contaminants in bile of fish exposed to wastewater treatment works effluents. Environmental Science & Technology, 2005, 39(8): 2461–2471

[8] Brown J N, Paxéus N, Förlin L, Larsson D G. Variations in bioconcentration of human pharmaceuticals from sewage effluents into fish blood plasma. Environmental Toxicology and Pharmacology, 2007, 24(3): 267–274

[9] Corcoran J, Winter M J, Tyler C R. Pharmaceuticals in the aquatic environment: a critical review of the evidence for health effects in fish. Critical Reviews in Toxicology, 2010, 40(4): 287–304

[10] Brooks B W, Chambliss C K, Stanley J K, Ramirez A, Banks K E, Johnson R D, Lewis R J. Determination of select antidepressants in fish from an effluent-dominated stream. Environmental Toxicology and Chemistry, 2005, 24(2): 464–469

[11] Ramirez A J, Mottaleb M A, Brooks B W, Chambliss C K. Analysis of pharmaceuticals in fish using liquid chromatography-tandem mass spectrometry. Analytical Chemistry, 2007, 79(8): 3155–3163

[12] Azzouz A, Souhail B, Ballesteros E. Determination of residual pharmaceuticals in edible animal tissues by continuous solid-phase extraction and gas chromatography-mass spectrometry. Talanta, 2011, 84(3): 820–828

[13] K’oreje K O, Demeestere K, de Wispelaere P, Vergeynst L, Dewulf J, van Langenhove H. From multi-residue screening to target analysis of pharmaceuticals in water: development of a new approach based on magnetic sector mass spectrometry and application in the Nairobi River basin, Kenya. Science of the Total Environment, 2012, 437: 153–164

[14] Kleywegt S, Pileggi V, Yang P, Hao C, Zhao X, Rocks C, Thach S, Cheung P, Whitehead B. Pharmaceuticals, hormones and bisphenol A in untreated source and finished drinking water in Ontario, Canada—occurrence and treatment efficiency. Science of the Total Environment, 2011, 409(8): 1481–1488

[15] Osorio V, Marcé R, Pérez S, Ginebreda A, Cortina J L, Barceló D. Occurrence and modeling of pharmaceuticals on a sewage-impacted Mediterranean river and their dynamics under different hydrological conditions. Science of the Total Environment, 2012, 440: 3–13

[16] Wang L, Ying G G, Zhao J L, Yang X B, Chen F, Tao R, Liu S, Zhou L J. Occurrence and risk assessment of acidic pharmaceuticals in the Yellow River, Hai River and Liao River of north China. Science of the Total Environment, 2010, 408(16): 3139–3147

[17] Beyer J, Jonsson G, Porte C, Krahn M M, Ariese F. Analytical methods for determining metabolites of polycyclic aromatic hydrocarbon (PAH) pollutants in fish bile: a review. Environmental Toxicology and Pharmacology, 2010, 30(3): 224–244

[18] Pettersson M, Adolfsson-Erici M, Parkkonen J, Förlin L, Asplund L. Fish bile used to detect estrogenic substances in treated sewage water. Science of the Total Environment, 2006, 366(1): 174–186

[19] Kallio J M, Lahti M, Oikari A, Kronberg L. Metabolites of the aquatic pollutant diclofenac in fish bile. Environmental Science & Technology, 2010, 44(19): 7213–7219

[20] Mehinto A C, Hill E M, Tyler C R. Uptake and biological effects of environmentally relevant concentrations of the nonsteroidal anti-inflammatory pharmaceutical diclofenac in rainbow trout (Oncorhynchus mykiss). Environmental Science & Technology, 2010, 44(6): 2176–2182

[21] Ramirez A J, Brain R A, Usenko S, Mottaleb M A, O’Donnell J G, Stahl L L, Wathen J B, Snyder B D, Pitt J L, Perez-Hurtado P, Dobbins L L, Brooks B W, Chambliss C K. Occurrence of pharmaceuticals and personal care products in fish: results of a national pilot study in the United States. Environmental Toxicology and Chemistry, 2009, 28(12): 2587–2597

[22] World Health Organization. Joint FAO/WHO Expert Meeting to Review Toxicological and Health Aspects of Bisphenol A: Summary Report including Report of Stakeholder Meeting on Bisphenol A. Ottawa, Canada: World Health Organization, 2010

[23] Ackerman L K, Noonan G O, Heiserman W M, Roach J A, Limm W, Begley T H. Determination of bisphenol A in U.S. infant formulas: updated methods and concentrations. Journal of Agricultural and Food Chemistry, 2010, 58(4): 2307–2313

[24] Cao X L, Corriveau J, Popovic S. Levels of bisphenol A in canned soft drink products in Canadian markets. Journal of Agricultural and Food Chemistry, 2009, 57(4): 1307–1311

[25] Noonan G O, Ackerman L K, Begley T H. Concentration of bisphenol A in highly consumed canned foods on the U.S. market. Journal of Agricultural and Food Chemistry, 2011, 59(13): 7178–7185

[26] Schecter A, Malik N, Haffner D, Smith S, Harris T R, Paepke O, Birnbaum L. Bisphenol A (BPA) in U.S. food. Environmental Science & Technology, 2010, 44(24): 9425–9430

[27] Ye X, Kuklenyik Z, Needham L L, Calafat A M. Measuring environmental phenols and chlorinated organic chemicals in breast milk using automated on-line column-switching-high performance liquid chromatography-isotope dilution tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 2006, 831(1–2): 110–115

[28] Zhang Z, Alomirah H, Cho H S, Li Y F, Liao C, Minh T B, Mohd M A, Nakata H, Ren N, Kannan K. Urinary bisphenol A concentrations and their implications for human exposure in several Asian countries. Environmental Science & Technology, 2011, 45(16): 7044–7050

[29] Kang J H, Kondo F, Katayama Y. Human exposure to bisphenol A. Toxicology, 2006, 226(2–3): 79–89

[30] U.S. Census Bureau. Health & Nutrition: Food Consumption and Nutrition, Per Capita Consumption of Major Food Commodities.

[31] Walpole S C, Prieto-Merino D, Edwards P, Cleland J, Stevens G, Roberts I. The weight of nations: an estimation of adult human biomass. BMC Public Health, 2012, 12(1): 439–444

[32] European Food Safety Authority. Opinion of the scientific panel on food additives, flavourings, processing aids and materials in contact with food on a request from the commission related to 2,2-bis(4-hydroxyphenyl) propane (bisphenol A). EFSA Journal, 2006, 428: 1–75

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