Behavior of aqueous stable colloidal nano-C₆₀ aggregates exposed to TX100 micelles under different environmental conditions

Abstract

C₆₀, as one of carbon nanomaterials widely used in various fields, could be released into the water environment thus exerting some potential health risks to human beings. This work examined the behavior of aqueous stable colloidal nano-C₆₀ (nC₆₀) aggregates under different environmental conditions including Polyethylene glycol octylphenol ether (TX100) micelles concentration, pH, and reaction time when exposed to TX100 micelles. Results show that the nC₆₀ aggregates became more dispersive and restored the capability of generating the singlet oxygen when exposed to TX100 micelles. With the increase of TX100 concentration, smaller average size of nC₆₀ aggregates was observed in dynamic light scattering (DLS) analysis, the fluorescence intensity of TX100 was more quenched by nC₆₀ aggregates, and the kinetic rate constant of generating the singlet oxygen for nC₆₀ aggregates was improved. The mean size of nC₆₀ aggregates in the presence of TX100 had no obvious variations when the pH ranged from 4 to 8. The longer reaction time between nC₆₀ aggregates and TX100 led to a higher kinetic rate constant of generating the singlet oxygen. Collective data suggest that variations in physicochemical properties of nC₆₀ aggregates are strongly dependent on the surrounding media under different environmental conditions and directly govern nC₆₀’s transport behavior and potential toxicity.

Keywords

nano-C₆₀ (nC₆₀) aggregates / photochemical reactivity / artificial biological membrane

References

[1] Lens M. Recent progresses in application of fullerenes in cosmetics. Recent Patents on Biotechnology, 2011, 5(2): 67–73

[2] Lin C M, Lu T Y. C₆₀ fullerene derivatized nanoparticles and their application to therapeutics. Recent Patents on Nanotechnology, 2012, 6(2): 105–113

[3] Nierengarten J F. Chemical modification of C₆₀ for materials science applications. New Journal of Chemistry, 2004, 28(10): 1177–1191

[4] Kroto H W, Allaf A W, Balm S P. C₆₀: buckminsterfullerene. Chemical Reviews, 1991, 91(6): 1213–1235

[5] Jafvert C T, Kulkarni P P. Buckminsterfullerene’s (C₆₀) octanol-water partition coefficient (K_ow) and aqueous solubility. Environmental Science & Technology, 2008, 42(16): 5945–5950

[6] Fortner J D, Lyon D Y, Sayes C M, Boyd A M, Falkner J C, Hotze E M, Alemany L B, Tao Y J, Guo W, Ausman K D, Colvin V L, Hughes J B. C₆₀ in water: nanocrystal formation and microbial response. Environmental Science & Technology, 2005, 39(11): 4307–4316

[7] Sayes C M, Fortner J D, Guo W, Lyon D, Boyd A M, Ausman K D, Tao Y J, Sitharaman B, Wilson L J, Hughes J B, West J L, Colvin V L. The differential cytotoxicity of water-soluble fullerenes. Nano Letters, 2004, 4(10): 1881–1887

[8] Song M, Yuan S, Yin J, Wang X, Meng Z, Wang H, Jiang G. Size-dependent toxicity of nano-C₆₀ aggregates: more sensitive indication by apoptosis-related Bax translocation in cultured human cells. Environmental Science & Technology, 2012, 46(6): 3457–3464

[9] Lee J, Fortner J D, Hughes J B, Kim J H. Photochemical production of reactive oxygen species by C₆₀ in the aqueous phase during UV irradiation. Environmental Science & Technology, 2007, 41(7): 2529–2535

[10] Fortner J D, Lyon D Y, Sayes C M, Boyd A M, Falkner J C, Hotze E M, Alemany L B, Tao Y J, Guo W, Ausman K D, Colvin V L, Hughes J B. C₆₀ in water: nanocrystal formation and microbial response. Environmental Science & Technology, 2005, 39(11): 4307–4316

[11] Brant J, Lecoanet H, Hotze M, Wiesner M. Comparison of electrokinetic properties of colloidal fullerenes (n-C₆₀) formed using two procedures. Environmental Science & Technology, 2005, 39(17): 6343–6351

[12] Xie B, Xu Z H, Guo W H, Li Q L. Impact of natural organic matter on the physicochemical properties of aqueous C₆₀ nanoparticles. Environmental Science & Technology, 2008, 42(8): 2853–2859

[13] Chang X J, Vikesland P J. Effects of carboxylic acids on nC₆₀ aggregate formation. Environmental Pollution, 2009, 157(4): 1072–1080

[14] Chen K L, Elimelech M. Influence of humic acid on the aggregation kinetics of fullerene (C₆₀) nanoparticles in monovalent and divalent electrolyte solutions. Journal of Colloid and Interface Science, 2007, 309(1): 126–134

[15] Hungerbuehler H, Guldi D M, Asmus K D. Incorporation of C₆₀ into artificial lipid membranes. Journal of the American Chemical Society, 1993, 115(8): 3386–3387

[16] Andrievsky G V, Kosevich M V, Vovk M, Shelkovsky V S, Vashchenko L A. On the production of an aqueous colloidal solution of fullerenes. Journal of the Chemical Society. Chemical Communications, 1995, 12(12): 1281–1282

[17] Brant J A, Labille J, Bottero J Y, Wiesner M R. Characterizing the impact of preparation method on fullerene cluster structure and chemistry. Langmuir, 2006, 22(8): 3878–3885

[18] Zhang B, Cho M, Hughes J B, Kim J H. Translocation of C₆₀ from aqueous stable colloidal aggregates into surfactant micelles. Environmental Science & Technology, 2009, 43(24): 9124–9129

[19] Deguchi S, Alargova R G, Tsujii K. Stable dispersions of fullerenes, C60 and C70, in water. Preparation and characterization. Langmuir, 2001, 17(19): 6013–6017

[20] Mchedlov-Petrossyan N O, Klochkov V K, Andrievsky G V. Colloidal dispersions of fullerene C₆₀ in water: some properties and regularities of coagulation by electrolytes. Journal of the Chemical Society, Faraday Transactions, 1997, 93(24): 4343–4346

[21] Kumbhakar M, Goel T, Mukherjee T, Pal H. Role of micellar size and hydration on solvation dynamics: a temperature dependent study in Triton-X-100 and Brij-35 micelles. Journal of Physical Chemistry B, 2004, 108(50): 19246–19254

[22] Kumbhakar M, Nath S, Mukherjee T, Pal H. Solvation dynamics in triton-X-100 and triton-X-165 micelles: effect of micellar size and hydration. The Journal of Chemical Physics, 2004, 121(12): 6026–6033

[23] Behera K, Dahiya P, Pandey S. Effect of added ionic liquid on aqueous Triton X-100 micelles. Journal of Colloid and Interface Science, 2007, 307(1): 235–245

[24] Kumbhakar M, Goel T, Mukherjee T, Pal H. Nature of the water molecules in the palisade layer of a triton X-100 micelle in the presence of added salts: a solvation dynamics study. Journal of Physical Chemistry B, 2005, 109(29): 14168–14174

[25] Das P, Mallick A, Purkayastha P, Haldar B, Chattopadhyay N. Fluorescence resonance energy transfer from TX-100 to 3-acetyl-4-oxo-6,7-dihydro-12 H-indolo-[2,3-a] quinolizine in premicellar and micellar environments. Journal of Molecular Liquids, 2007, 130(1–3): 48–51

[26] Haag W R, Hoigne J, Gassman E, Braun A MSinglet oxygen in surface waters-Part I: furfuryl alcohol as a trapping agent. Chemosphere, 1984, 13(5–6): 631–640

[27] Li Y, Zhang W, Niu J F, Chen Y S. Mechanism of photogenerated reactive oxygen species and correlation with the antibacterial properties of engineered metal-oxide nanoparticles. ACS Nano, 2012, 6(6): 5164–5173

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 21177084), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (2010.40th), Shanghai Jiao Tong University Medical and Engineering Crossing Research Fund, Shanghai Jiao Tong University SMC-B Research Fund, and Intercollegiate cooperative project supported by Shanghai Jiaotong University(2013).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg