Organic and inorganic phosphorus uptake by bacteria in a plug-flow microcosm

Abstract

Phosphorus (P) is a vital nutrient for sustaining natural water productivity. Both particulate and dissolved forms of organic and inorganic P are potentially important sources of bioavailable P for primary and secondary producers. A microcosm system to imitate the bacterial community in Plym river sediment and pore water is described and bacterial uptake rates for inorganic and organic phosphorus are presented in this paper. The aim of this study was to investigate the uptake of two organic phosphorus compounds (phytic acid and D-glucose-6-phosphate) by freshwater bacteria. The bioreactors comprise glass columns packed with two types of small glass beads on which bacterial biofilm can develop. The glass beads with different porosity were introduced to simulate River SPM. The selected P compounds spiked into the inflow of the microcosm, and measured the step change of P concentration in the outflow to investigate the behavior of bacterial uptake of nutrients. The results showed that organic phosphorus was converted into inorganic phosphorus but the conversion rate depended on the type of phosphorus species. One experiment suggested that phytic acid (refractory) could displace phosphate from the biofilm surface; the other experiment showed that D-glucose-6-phosphate (labile) could be hydrolysed and utilized easily by the bacteria. The results also suggested that bacteria might break down the C-P bonds to utilize the carbon. Further experiments should investigate the effect of varying the C:N:P ratio in the microcosm system to determine which nutrient limits bacteria uptake.

Keywords

organic phosphorus / bacteria / uptake

References

[1] de Groot C J, Golterman H L. On the presence of organic phosphate in some camargue sediments-evidence for the importance of phytate. Hydrobiologia, 1993, 252(1): 117–126

[2] Turner B L, Papházy M J, Haygarth P M, McKelvie I D. Inositol phosphates in the environment. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 2002, 357(1420): 449–469

[3] Cotner J B, Wetzel R G. Uptake of dissolved inorganic and organic phosphorus compounds by phytoplankton and bacterioplankton. Limnology and Oceanography, 1992, 37(2): 232–243

[4] Nicholson D, Dyhrman S, Chavez F, Paytan A. Alkaline phosphatase activity in the phytoplankton communities of Monterey Bay and San Fransisco Bay. Limnology and Oceanography, 2006, 51(2): 874–883

[5] Vanderzee C, Roevros N, Chou L. Phosphorus speciation, transformation and retention in the Scheldt estuary (Belgium/The Netherlands) from the freshwater tidal limits to the North Sea. Marine Chemistry, 2007, 106(1-2): 76–91

[6] Cotner J B, Ammerman J W, Peele E R, Bentzen E. Phosphorus-limited bacterioplankton growth in the Sargasso Sea. Aquatic Microbial Ecology, 1997, 13(2): 141–149

[7] Labry C, Delmas D, Herbland A. Phytoplankton and bacterial alkaline phosphatase activities in relation to phosphate and DOP availability within the Gironde plume waters (Bay of Biscay). Journal of Experimental Marine Biology and Ecology, 2005, 318(2): 213–225

[8] Monbet P, McKelvie I D, Saefumillah A, Worsfold P J. A protocol to assess the enzymatic release of dissolved organic phosphorus species in waters under environmentally relevant conditions. Environmental Science & Technology, 2007, 41(21): 7479–7485

[9] Heath R T. Microbial Turnover of Organic Phosphorus in Aquatic Systems. London: CABI Publishing, 2005, 133–204

[10] Drummond L, Maher W. Determination of phosphorus in aqueous solution via formation of the phosphoantimonylmolybdenum blue complex. Re-examination of optimum conditions for the analysis of phosphate. Analytica Chimica Acta, 1995, 302(1): 69–74

[11] McKelvie I D. Separation, Preconcentration and Speciation of Organic Phosphorus in Environmental Samples. London: CABI Publishing, 2005, 1-20

[12] Miettinen I T, Vartiainen T, Martikainen P J. Phosphorus and bacterial growth in drinking water. Applied and Environmental Microbiology, 1997, 63(8): 3242–3245

[13] Sathasivan A, Ohgaki S, Yamamoto K, Kamiko N. Role of inorganic phosphorus in controlling regrowth in water distribution system. Water Science and Technology, 1997, 35(8): 37–44

[14] Appenzeller B M R, Batté M, Mathieu L, Block J C, Lahoussine V, Cavard J, Gatel D.Effect of adding phosphate to drinking water on bacterial growth in slightly and highly corroded pipes. Water Research, 2001, 35(4): 1100–1105

[15] Lucena F, Frias J, Ribas F. A new dynamic approach to the determination of biodegradable dissolved organic carbon in water. Environmental Science & Technology, 1990, 12(4): 343–347

[16] Frias J, Ribas F, Lucena F. A method for the measurement of biodegradable organic carbon in waters. Water Research, 1992, 26(2): 255–258

[17] Kaplan L A, Newbold J D. Measurement of streamwater biodegradable dissolved organic carbon with a plug-flow bioreactor. Water Research, 1995, 29(12): 2696–2706

[18] Søndergaard M, Worm J. Measurement of biodegradable dissolved organic carbon (BDOC) in lake water with a bioreactor. Water Research, 2000, 35(10): 2505–2513

[19] Badr E A, Tappin A, Achterberg E.Distributions and seasonal variability of dissolved organic nitrogen in two estuaries in SW England. Marine Chemistry, 2008, 110(3-4): 153–164

[20] Kirkman H N, Gaetani G F. Regulation of glucose-6-phosphate dehydrogenase in human erythrocytes. Journal of Biological Chemistry, 1986, 261(9): 4033–4038

[21] Steer T E, Gee J N, Johnson I T, Gibson G R. Biodiversity of human faecal bacteria isolated from phytic acid enriched chemostat fermenters. Current Issues in Intestinal Microbiology, 2004, 5(2): 23–39

[22] Taylor S A, Steer T E, Gibson G R. Diet, bacteria and colonic cancer. Nutrition & Food Science, 1999, 99(4): 187–193

[23] Grases F, March P. Determination of phytic acid based on inhibition of crystalline growth of calcium oxalate monohydrate. Analytica Chimica Acta, 1989, 219(1): 89–95

[24] Monbet P, McKelvie I D, Worsfold P J. Dissolved organic phosphorus speciation in the waters of the Tamar estuary (SW England). Geochimica et Cosmochimica Acta, 2009, 73(4): 1027–1038

[25] Suzumura M, Kamatani A. Mineralization of inositol hexaphosphate in aerobic and anaerobic marine sediments: implications for the phosphorus cycle. Geochimica et Cosmochimica Acta, 1995, 59(5): 1021–1026

[26] Celi L, Lamacchia S, Ajmone-Marsan F, Barberis E. Interaction of inositol hexaphosphate on clays: adsorption and charging phenomena. Soil Science, 1999, 164(8): 574–585

[27] Anderson G, Williams E G, Moir J O. A comparison of the sorption of inorganic phosphate and inositol-hexaphosphate by six acid soils. Journal of Soil Science, 1974, 25(1): 51–62

[28] Badr E A. Environmental assessment of biogeochemical cycling of dissolved organic carbon (DOC) and nitrogen (DON) in natural waters. Dissertation for the Doctoral Degree. Plymouth: University of Plymouth, 2005

[29] Norrman B. Filtration of Water Samples for Doc Studies. Marine Chemistry, 1993, 41(1-3): 239–242

[30] Amako K, Umeda A.An improved method for observation of bacterial growth using the scanning electron microscope. Journal of Electron Microscopy, 1977, 26(2): 155–159

[31] Miles A A, Misra S S, Irwin J O. The estimation of the bactericidal power of the blood. Journal of Hygiene, 1938, 38(06): 732–749

[32] Prescott L M, Harley J P, Klein D A. Microbiology. London: McGraw-Hill London, 1999, 98-160

[33] Omaka N O. Flow injection techniques for investigating the biogeochemistry of nutrients in natural waters. Dissertation for the Doctoral Degree. Plymouth: University of Plymouth, 2005

Acknowledgements

The authors would like to thank Prof. Paul Worsfold, Dr. Miranda Keith-Roach, Dr Alan Tappin and Prof. Ian McKelvie for the help and thanks to the National Water Pollution Control Special Project for fund (No. 2008ZX07313-004-05c) and Beijing Normal University Youth Science Foundation (No. 105503GK).

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