Decolorization of azo dyes by a salt-tolerant Staphylococcus cohnii strain isolated from textile wastewater

Abstract

The salt-tolerant Staphylococcus cohnii strain, isolated from textile wastewater, has been found effective on decolorizing several kinds of azo dyes with different structures. The optimal conditions for azo dye acid red B (ARB) decolorization by S. cohnii were determined to be pH= 7.0 and 30°C. The decolorization efficiency increased with the increase of the salinity concentration, and around 90% of ARB (100 mg·L^-1) could be decolorized in 24 h when the salinity concentration was up to 50 g·L^-1. Moreover, the strain could still decolorize 19% of ARB in 24 h even when the NaCl concentration was increased to 150 g·L^-1. Meanwhile, the dependence of the specific decolorization rate by S. cohnii on the ARB concentration could be described with Michaelis-Menten kinetics (K_m = 585.7 mg·L^-1, V_max = 109.8 mg·g cell^-1·h^-1). The addition of quinone redox mediator, named 2-hydroxy-1,4-naphthoquinone and anthraquinone-2,6-disulfonate, significantly accelerated the decolorization performance of S. cohnii. Furtherly, the activities of azoreductase (0.55 μmol·mg protein^-1·min^-1) and Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase (8.9 μmol·mg protein^-1·min^-1) have been observed in the crude cell extracts of S. cohnii. The decolorization products of ARB were analyzed by HPLC-MS, and the results indicated the reductive pathway was responsible for azo dye decolorization by S. cohnii.

Keywords

Staphylococcus cohnii / decolorization / salt / azoreductase / Nicotineamide adenine dinucleotide-dichlorophenol indophenol (NADH-DCIP) reductase

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Acknowledgements

This research was supported by Fujian Province Key Project of Science and Technology (No. 2011N0036) and Xiamen Science and Technology Plan Project (No. 3502Z20113034).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg