Effect of different gas releasing methods on anaerobic fermentative hydrogen production in batch cultures

Abstract

Decreasing hydrogen partial pressure can not only increase the activity of the hydrogen enzyme but also decrease the products inhibition, so it is an appropriate method to enhance the fermentative hydrogen production from anaerobic mixed culture. The effect of biogas release method on anaerobic fermentative hydrogen production in batch culture system was compared, i.e., Owen method with intermediately release, continuous releasing method, and continuous releasing+ CO₂ absorbing. The experimental results showed that, at 35°C, initial pH 7.0 and glucose concentration of 10 g·L^-1, the hydrogen production was only 28 mL when releasing gas by Owen method, while it increased two times when releasing the biogas continuously. The cumulative hydrogen production could reach 155 mL when carbon dioxide in the gas stream was continuously absorbed by 1 mol·L^-1 NaOH. The results showed that acetate was dominated, accounting for 43% in the dissolved fermentation products in Owen method, whereas the butyrate predominated and reached 47%–53% of the total liquid end products when releasing gas continuously. It is concluded that the homoacetogenesis could be suppressed when absorbing CO₂ in the gas phase in fermentative hydrogen production system.

Keywords

batch fermentation / hydrogen production / biogas releasing / hydrogen pressure / homoacetogenesis

References

[1] Hallenbeck P C, Ghosh D. Advances in fermentative biohydrogen production: the way forward? Trends in Biotechnology, 2009, 27(5): 287-297

[2] Lee H S, Vermaas W F J, Rittmann B E. Biological hydrogen production: prospects and challenges. Trends in Biotechnology, 2010, 28(5): 262-271

[3] Li J, Zheng G, He J, Chang S, Qin Z. Hydrogen-producing capability of anaerobic activated sludge in three types of fermentations in a continuous stirred-tank reactor. Biotechnology Advance, 2009, 27(5): 573-577

[4] Lee H S, Krajmalinik-Brown R, Zhang H, Rittmann B E. An electron-flow model can predict complex redox reactions in mixed-culture fermentative BioH₂: Microbial ecology evidence. Biotechnology and Bioengineering, 2009, 104(4): 687-697

[5] Valdez-Vazquez I, Ríos-Leal E, Carmona-Martínez A, Muñoz-Páez K M, Poggi-Varaldo H M. Improvement of biohydrogen production from solid wastes by intermittent venting and gas flushing of batch reactors headspace. Environmental Science & Technology, 2006, 40(10): 3409-3415

[6] Owen W F, Stuckey D C, Healy J B Jr, Young L Y, McCarty P L. Bioassay for monitoring biochemical methane potential and anaerobic toxicity. Water Research, 1979, 13(6): 485-492

[7] Logan B E, Oh S E, Kim I S, van Ginkel S. Biological hydrogen production measured in batch anaerobic respirometers. Environmental Science & Technology, 2002, 36(11): 2530-2535

[8] Tanisho S, Kuromoto M, Kadokura N. Effect of CO₂ removal on hydrogen production by fermentation. International Journal of Hydrogen Energy, 1998, 23(7): 559-563

[9] Oh S E, van Ginkel S, Logan B E. The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. Environmental Science & Technology, 2003, 37(22): 5186-5190

[10] Ren N, Guo W, Wang X, Xiang W, Liu B, Wang X, Wang X, Ding J, Chen Z. Effects of different pretreatment methods on fermentation types and dominant bacteria for hydrogen production. International Journal of Hydrogen Energy, 2008, 33(16): 4318-4324

[11] APHA. Standard Methods for Examination of Water and Wastewater. 19th ed. Washington D C: American Public Health Association, 1998

[12] Park W, Hyun S H, Oh S E, Logan B E, Kim I S. Removal of headspace CO₂ increases biological hydrogen production. Environmental Science & Technology, 2005, 39(12): 4416-4420

[13] Minton N P, Clarke D J. Clostridia—Biotechnology Handbook. Vol. 3. New York: Plenum, 1989

[14] Ohwaki K, Hungate R E. Hydrogen utilization by clostridia in sewage sludge. Applied and Environmental Microbiology, 1977, 33(6): 1270-1274

[15] Ragsdale S W, Pierce E.Acetogenesis and the Wood-Ljungdahl pathway of CO₂ fixation. Biochimica et Biophysica Acta, 2008, 1784(12): 1873-1898

[16] Lay J J, Lee Y J, Noike T. Feasibility of biological hydrogen production from organic fraction of municipal solid waste. Water Research, 1999, 33(11): 2579-2586

[17] Hawkes F R, Dinsdale R, Hawkes D L, Hussy I. Sustainable fermentative hydrogen production: challenges for process optimization. International Journal of Hydrogen Energy, 2002, 27(11-12): 1339-1347

[18] Hu B, Chen S L. Pretreatment of methanogenic granules for immobilized hydrogen fermentation. International Journal of Hydrogen Energy, 2007, 32(15): 3266-3273

[19] Wang J L, Wan W. Comparison of different pretreatment methods for enriching hydrogen-producing bacteria from digested sludge. International Journal of Hydrogen Energy, 2008, 33(12): 2934-2941

[20] Chen C C, Lin C Y, Lin M C. Acid-base enrichment enhances anaerobic hydrogen production process. Applied Microbiology and Biotechnology, 2002, 58(2): 224-228

[21] Hafez H, Nakhla G, Naggar H, Elbeshbishy E, Baghchehsaraee B. Effect of organic loading on a novel hydrogen bioreactor. International Journal of Hydrogen Energy, 2010, 35(1): 81-92

[22] Lee H S, Rittmann B E. Evaluation of metabolism using stoichiometry in fermentative biohydrogen. Biotechnology and Bioengineering, 2009, 102(3): 749-758

[23] Oh S E, Zuo Y, Zhang H, Guiltinan M J, Logan B E, Regan J M. Hydrogen production by Clostridium acetobutylicum ATCC 824 and megaplasmid-deficient mutant M5 evaluated using a large headspace volume technique. International Journal of Hydrogen Energy, 2009, 34(23): 9347-9353

Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grant No. 51178136), State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (2010DX06), the National High Technology Research and Development Program of China (No. 2006AA05Z109) and Harbin Science and Technology Bureau (2009RFXXS004).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg