Effects of hydraulic retention time on nitrification activities and population dynamics of a conventional activated sludge system

Abstract

The effects of hydraulic retention time (HRT) on the nitrification activities and population dynamics of a conventional activated sludge system fed with synthetic inorganic wastewater were investigated over a period of 260 days. When the HRT was gradually decreased from 30 to 5 h, the specific ammonium-oxidizing rates (SAOR) varied between 0.32 and 0.45 kg \mathrm{N}{\mathrm{H}}_{\mathrm{4}}^{+}-\mathrm{N} (kg mixed liquor suspended solids (MLSS)·d)^-1, and the specific nitrate-forming rates (SNFR) increased from 0.11 to 0.50 kg \mathrm{N}{\mathrm{O}}_{\mathrm{3}}^{-}-\mathrm{N} (kg MLSS·d)^-1, showing that the decrease in HRT led to a significant increase in the nitrite oxidation activity. According to fluorescence in situ hybridization (FISH) analysis results, the proportion of ammonia-oxidizing bacteria (AOBs) among the total bacteria decreased from 33% to 15% with the decrease in HRT, whereas the fraction of nitrite-oxidizing bacteria (NOBs), particularly the fast-growing Nitrobacter sp., increased significantly (from 4% to 15% for NOBs and from 1.5% to 10.6% for Nitrobacter sp.) with the decrease in HRT, which was in accordance with the changes in SNFR. A short HRT favored the relative growth of NOBs, particularly the fast-growing Nitrobacter sp., in the conventional activated sludge system.

Keywords

ammonia-oxidizing bacteria / hydraulic retention time / nitrification activity / nitrite-oxidizing bacteria / population dynamics

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Acknowledgements

Funding for this research was provided by the National Natural Science Foundation of China (Grant No. 20921140094) and the the Knowledge Innovation Project of Chinese Academy of Sciences (KSCX2-YW-G-054).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg