Influence of influent on anaerobic ammonium oxidation in an Expanded Granular Sludge Bed-Biological Aerated Filter integrated system

Abstract

Shortcut nitrification-denitrification, anaerobic ammonium oxidation (ANAMMOX), and methanogenesis have been successfully coupled in an Expanded Granular Sludge Bed-Biological Aerated Filter (EGSB-BAF) integrated system. As fed different synthetic wastewater with chemical oxygen demand (COD) of 300–1200 mg·L^-1 and {NH}_4^{+}-N of 30–120 mg·L^-1 at the outer recycle ratio of 200%, the influence of influent on ANAMMOX in the integrated system was investigated in this paper. The experimental results showed that higher COD concentration caused an increase in denitrification and methanogenesis but a decrease in ANAMMOX; however, when an influent with the low concentration of COD was used, the opposite changes could be observed. Higher influent {NH}_4^{+}-N concentration favored ANAMMOX when the COD concentration of influent was fixed. Therefore, low COD/{NH}_4^{+}-N ratio would decrease competition for nitrite between ANAMMOX and denitrification, which was favorable for reducing the negative effect of organic COD on ANAMMOX. The good performance of the integrated system indicated that the bacterial community of denitrification, ANAMMOX, and methanogenesis could be dynamically maintained in the sludge of EGSB reactor for a certain range of influent.

Keywords

Expanded Granular Sludge Bed-Biological Aerated Filter (EGSB-BAF) integrated system / the influence of influent / anaerobic ammonium oxidation (ANAMMOX) / shortcut nitrification-denitrification / methano-genesis

References

[1] Chamchoi N, Nitisoravut S, Schmidt J E. Inactivation of ANAMMOX communities under concurrent operation of anaerobic ammonium oxidation (ANAMMOX) and denitrification. Bioresource Technology, 2008, 99(9): 3331-3336

[2] Dong X, Tollner E W. Evaluation of Anammox and denitrification during anaerobic digestion of poultry manure. Bioresource Technology, 2003, 86(2): 139-145

[3] Molinuevo B, García M C, Karakashev D, Angelidaki I. Anammox for ammonia removal from pig manure effluents: effect of organic matter content on process performance. Bioresource Technology, 2009, 100(7): 2171-2175

[4] Thamdrup B, Dalsgaard T. Production of N₂ through anaerobic ammonium oxidation coupled to nitrate reduction in marine sediments. Applied and Environmental Microbiology, 2002, 68(3): 1312-1318

[5] Zang D J. The integration of methanogenesis with denitrification and anaerobic ammonium oxidation in an expanded granular sludge bed reactor. Journal of Environmental Sciences (China), 2003, 15(3): 423-432

[6] Ahn Y H, Hwang I S, Min K S. ANAMMOX and partial denitritation in anaerobic nitrogen removal from piggery waste. Water science and technology, 2004, 49(5-6): 145-153

[7] Wang J L, Kang J. The characteristics of anaerobic ammonium oxidation (ANAMMOX) by granular sludge from an EGSB reactor. Process Biochemistry, 2005, 40(5): 1973-1978

[8] Kalyuzhnyi S, Gladchenko M, Mulder A, Versprille B. DEAMOX—new biological nitrogen removal process based on anaerobic ammonia oxidation coupled to sulphide-driven conversion of nitrate into nitrite. Water Research, 2006, 40(19): 3637-3645

[9] Sumino T, Isaka K, Ikuta H, Saiki Y, Yokota T. Nitrogen removal from wastewater using simultaneous nitrate reduction and anaerobic ammonium oxidation in single reactor. Journal of Bioscience and Bioengineering, 2006, 102(4): 346-351

[10] Pathak B K, Kazama F, Saiki Y, Sumino T. Presence and activity of anammox and denitrification process in low ammonium-fed bioreactors. Bioresource Technology, 2007, 98(11): 2201-2206

[11] Pathak B K, Kazama F, Tanaka Y, Mori K, Sumino T. Quantification of anammox populations enriched in an immobilized microbial consortium with low levels of ammonium nitrogen and at low temperature. Applied Microbiology and Biotechnology, 2007, 76(5): 1173-1179

[12] Kalyuzhnyi S V, Gladchenko M A, Kang H, Mulder A, Versprille A. Development and optimisation of VFA driven DEAMOX process for treatment of strong nitrogenous anaerobic effluents. Water science and technology, 2008, 57(3): 323-328

[13] Ruscalleda M, López H, Ganigué R, Puig S, Balaguer M D, Colprim J. Heterotrophic denitrification on granular anammox SBR treating urban landfill leachate. Water science and technology, 2008, 58(9): 1749-1755

[14] Udert K M, Kind E, Teunissen M, Jenni S, Larsen T A. Effect of heterotrophic growth on nitritation/anammox in a single sequencing batch reactor. Water science and technology, 2008, 58(2): 277-284

[15] Chen H, Liu S, Yang F, Xue Y, Wang T. The development of simultaneous partial nitrification, ANAMMOX and denitrification (SNAD) process in a single reactor for nitrogen removal. Bioresource Technology, 2009, 100(4): 1548-1554

[16] Xiao Y, Zeng G M, Yang Z H, Liu Y Sh, Ma Y H, Yang L, Wang R J, Xu ZhY. Coexistence of nitrifiers, denitrifiers and Anammox bacteria in a sequencing batch biofilm reactor as revealed by PCR-DGGE. Journal of Applied Microbiology, 2009, 106(2): 496-505

[17] Wang C C, Lee P H, Kumar M, Huang Y T, Sung S, Lin J G. Simultaneous partial nitrification, anaerobic ammonium oxidation and denitrification (SNAD) in a full-scale landfill-leachate treatment plant. Journal of Hazardous Materials, 2010, 175(1-3): 622-628

[18] Güven D, Dapena A, Kartal B, Schmid M C, Maas B, van de Pas-Schoonen K, Sozen S, Mendez R, Op den Camp H J, Jetten M S, Strous M, Schmidt I. Propionate oxidation by and methanol inhibition of anaerobic ammonium-oxidizing bacteria. Applied and Environmental Microbiology, 2005, 71(2): 1066-1071

[19] Strous M, Pelletier E, Mangenot S, Rattei T, Lehner A, Taylor M W, Horn M, Daims H, Bartol-Mavel D, Wincker P, Barbe V, Fonknechten N, Vallenet D, Segurens B, Schenowitz-Truong C, Médigue C, Collingro A, Snel B, Dutilh B E, Op den Camp H J, van der Drift C, Cirpus I, van de Pas-Schoonen K T, Harhangi H R, van Niftrik L, Schmid M, Keltjens J, van de Vossenberg J, Kartal B, Meier H, Frishman D, Huynen M A, Mewes H W, Weissenbach J, Jetten M S, Wagner M, Le Paslier D. Deciphering the evolution and metabolism of an anammox bacterium from a community genome. Nature, 2006, 440(7085): 790-794

[20] Kartal B, Rattray J, van Niftrik L A, van de Vossenberg J, Schmid M C, Webb R I, Schouten S, Fuerst J A, Damsté J S, Jetten M S, Strous M. Candidatus “Anammoxoglobus propionicus” a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria. Systematic and Applied Microbiology, 2007, 30(1): 39-49

[21] Kartal B, van Niftrik L, Rattray J, van de Vossenberg J L, Schmid M C, Sinninghe Damsté J, Jetten M S, Strous M. Candidatus ‘Brocadia fulgida’: an autofluorescent anaerobic ammonium oxidizing bacterium. FEMS Microbiology Ecology, 2008, 63(1): 46-55

[22] Rattray J E, Geenevasen J A, van Niftrik L, Rijpstra W I, Hopmans E C, Strous M, Schouten S, Jetten M S, Sinninghe Damsté J S. Carbon isotope-labelling experiments indicate that ladderane lipids of anammox bacteria are synthesized by a previously undescribed, novel pathway. FEMS Microbiology Letters, 2009, 292(1): 115-122

[23] Tang C J, Zheng P, Wang C H, Mahmood Q. Suppression of anaerobic ammonium oxidizers under high organic content in high-rate Anammox UASB reactor. Bioresource Technology, 2010, 101(6): 1762-1768

[24] Sabumon P C. Anaerobic ammonia removal in presence of organic matter: a novel route. Journal of Hazardous Materials, 2007, 149(1): 49-59

[25] Zhang D J, Yan Q, Zu B. Anaerobic ammonium oxidation, methanogenesis and shortcut nitrification-denitrification by EGSB-BAF integrated system. Research of Environmental Science,2009, 22(4): 467-472 (in Chinese)

[26] Ministry of Environmental protection of the People’s Republic of China. Water and exhausted water monitoring analysis method. 4th ed. Beijing: China Environmental Science Press, 2002 (in Chinese)

[27] Zu B, Zhang D J, Yan Q. Determination of 16S rRNA gene sequence for a new ANAMMOX bacterial species. Environmental Sciences, 2008, 29(2): 469-473 (in Chinese)

[28] IWA task group on mathematical modelling for design and operation of biological wasterwater treatment. Activated sludge models ASM1, ASM2, ASM2d and ASM3. IWA Publishing in its Scientific and Technical Report series, 2002, 10-24 (in Chinese)

[29] Yang Y, Zuo J E, Shen P, Gu X S. Influence of temperature, pH value and organic substance on activity of ANAMMOX sludge. Environmental Sciences, 2006, 27(4): 691-695 (in Chinese)

[30] Bernet N, Dangcong P, Delgenès J P, Moletta R. Nitrification at low oxygen concentration in biofilm reactor. Journal of Environmental Engineering ASCE, 2001, 127(3): 266-271

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