Spatial and seasonal variations in bacterial communities of the Yellow Sea by T-RFLP analysis

Abstract

Four typical coastal sites (rocky shore, sandy shore, mud flat shore, and artificial harbor) at the Yellow Sea were chosen to investigate the spatial and seasonal variations in bacterial communities. This was accomplished by using terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR amplified 16S rDNA fragments. Two kinds of tetrameric restriction enzymes, HhaI and MspI, were used in the experiment to depict the bacterial community diversity in different marine environments. It was found that the community compositions digested by the two enzymes separately were different. However, the results of bacterial community diversity derived from them were similar. The MDA analysis results of T-RFLP profiles coming from HhaI and MspI both exhibited a significant seasonal community shift for bacteria and a relatively low spatial variation among the four locations. With HhaI as the sample, the pair wise T-tests also revealed that variations were minor between each pair of marine environments, with R ranging from 0.198 to 0.349. However, the bacterial community structure in the mud flat site depicted a larger difference than each of the other three sites (R ranging from 0.282 to 0.349).

Keywords

terminal restriction fragment length polymorphism (T-RFLP) / bacterial community structure / marine microbial diversity / HhaI / MspI

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Acknowledgements

This research was supported by the 908 Special Program from State Oceanic Administration—Investigation and Evaluation on Marine Medicinal Organism Sources (No. 908-01-ST12).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg