Aerobic granulation of pure bacterial strain

Abstract

The objective of this study is to cultivate aerobic granules by pure bacterial strain, Bacillus thuringiensis, in a sequencing batch reactor. Stable granules sized 2.0–2.2 mm were formed in the reactor after a five-week cultivation. These granules exhibited excellent settling attributes, and degraded phenol at rates of 1.49 and 1.19 g phenol/(g VSS·d) at 250 and 1500 mg/L of phenol concentration, respectively. Confocal laser scanning microscopic test results show that Bacillus thuringiensis was distributed over the initial small aggregates, and the outer edge of the granule was away from the core regime in the following stage.

References

1. Beun J J, Hendriks A, Loosdrecht M C M van, Morgenroth E, Wilderer P A, Heijnen J J . Aerobic granulation in a sequencing batch reactor. Water Research, 1999, 33: 2283–2290. doi:10.1016/S0043-1354(98)00463-1
2. Peng D C, Bernet N, Delgenes J P, Moletta R . Aerobicgranular sludge–a case report. WaterResearch, 1999, 33: 890–893. doi:10.1016/S0043-1354(98)00443-6
3. Tay J H, Liu Q S, Liu Y . Microscopic observation of aerobic granulation in sequentialaerobic sludge blanket reactor. Journalof Applied Microbiology, 2001, 91: 168–175. doi:10.1046/j.1365-2672.2001.01374.x
4. Su K Z, Yu H Q . Formation and characterizationof aerobic granules in a sequencing batch reactor treating soybean-processingwastewater. Environmental Science &Technology, 2005, 39: 2818–2828. doi:10.1021/es048950y
5. Adav S S, Chen M Y, Lee D J, Ren N Q . Degradationof phenol by Acinetobactor strainisolated from aerobic granules. Chemosphere, 2007, 67: 1566–1572. doi:10.1016/j.chemosphere.2006.11.067
6. Adav S S, Chen M Y, Lee D J, Ren N Q . Degradationof phenol by aerobic granules and isolated yeast Candida tropicalis. Biotechnologyand Bioengineering, 2007, 96: 844–852. doi:10.1002/bit.21148
7. Adav S S, Lee D J, Ren N Q . Biodegradation of pyridine using aerobic granules inthe presence of phenol. Water Research, 2007, 41: 2903–2910. doi:10.1016/j.watres.2007.03.038
8. Jiang H L, Tay J H, Maszenan A M, Tay S T L . Bacterialdiversity and function of aerobic granules engineered in a sequencingbatch reactor for phenol degradation. Appliedand Environmental Microbiology, 2004, 70: 6767–6775. doi:10.1128/AEM.70.11.6767-6775.2004
9. Jiang H L, Tay J H, Maszenan A M, Tay S T L . Enhancedphenol biodegradation and aerobic granulation by two coaggregatingbacterial strains. Environmental Science &Technology, 2006, 40: 6137–6142. doi:10.1021/es0609295
10. Jiang H L, Tay S T L, Maszenan A M, Tay J H . Physiologicaltraits of bacterial strains isolated from phenol-degrading aerobicgranules. FEMS Microbiology Ecology, 2006, 57: 182–191. doi:10.1111/j.1574-6941.2006.00114.x
11. Jiang H L, Maszenan A M, Tay J H . Bioaugmentation of coexistence of two functionally similarbacterial strains in aerobic granules. Applied Microbiology and Biotechnology, 2007, 75: 1191–1200. doi:10.1007/s00253-007-0917-9
12. Adav S S, Lee D J . Physiological characterizationand interactions of isolates in phenol degrading aerobic granules. Applied Microbiology and Biotechnology, 2008. doi: . doi: 10.1007/s00253-008-1370-0
13. Chen M Y, Lee D J, Tay J H . Distributions of extracellular polymeric substances inaerobic granules. Applied Microbiologyand Biotechnology, 2007, 73: 1463–1469. doi:10.1007/s00253-006-0617-x
14. APHA. . TheStandard Methods for the Examination of Water and Wastewater, 20thed. Washington, DC: American Public Health Association, 1998 .
15. Tay J H, Liu Q S, Liu Y . Characteristics of aerobic granules grown on glucoseand acetate in sequential aerobic sludge blanket reactors. Environmental Technology, 2002, 23: 931–36. doi:10.1080/09593332308618363
16. Pinchuk I V, Bressollie P, Verneuil B, Fenet B, Sorokulova I B, Megraud F, Urdaci M C . In vitroanti-helicobacter pylori activityof the probiotic strain bacillus subtilis 3 is due to secretion of antibiotics. Antimicrobial Agents and Chemotherapy, 2001, 45: 3156–3161. doi:10.1128/AAC.45.11.3156-3161.2001
17. Park S C, Lee J R, Shin S O, Park Y, Lee S Y, Hahm K S . Characterization of a heat-stable protein with antimicrobial activityfrom Arabidopsis thaliana. Biochemical and Biophysical Research Communications, 2008. doi: . doi: 10.1016/j.bbrc. 2007.07.188
18. Naclerio G, Ricca E, Sacco M, Felic M . Antimicrobialactivity of a newly identified bacteriocin of bacillus cereus. Applied and Environmental Microbiology, 1993, 59: 4313–4316
19. Novotny J F, Perry J J . Characterization of bacteriocinsfrom two strains of Bacillus thermoleovorans, a thermophilic hydrocarbon-utilizing species. Applied and Environmental Microbiology, 1992, 58: 2393–2396
20. Kiaenhammer T R . Bacteriocins of lactic acid bacteria. Biochimie, 1988, 70: 337–349. doi:10.1016/0300-9084(88)90206-4