Inhibitive effects of three compositae plants on Microcystis aeruginosa

Abstract

Based on common phenomena of biochemical interaction between plants and microorganisms, the inhibitive effects of three common terrestrial compositae plants, namely Artemisia lavandulaefolia DC., Conyza canadensis (L.) Cronq., and Kalimeris indica (L.) Sch.-Bip. on the blue algae Microcystis aeruginosa was studied. Live compositae plants are co-cultivated with algae in two different inoculation doses for 10 days in 5-pools incubators, in order to exclude the influence of bacteria and nutrients. The results show that Artemisia lavandulaefolia DC has the most inhibitive potential among the three plants as evidenced by the most drastic decrease in optical density (OD₆₈₀) of the algae. The inhibition rate is 93.3% (with initial inoculation dose of 2.0 × 10⁶ Cells/mL) and 89.3% (with initial inoculation dose of 4.0 × 10⁶ Cells/mL) respectively on the 10^th day of cultivation. The average inhibition rate during the later half of the experiment is 0.76 (with initial inoculation dose of 2.0 × 10⁶ Cells/mL) and 0.71 (with initial inoculation dose of 4.0 × 10⁶ Cells/mL), respectively. Logistic model analysis shows that compositae plants such as A. lavandulaefolia DC. causes the reduction of the habitat's carrying capacity of algae. ANOVA analysis is used to determine the similarity and differences between every experimental group and an average inhibitive rate model is used to evaluate the inhibition effects. The results show that A. lavandulaefolia DC., which grow well in the aquatic environment, may have a great potential in controlling algae bloom in eutrophic water.

Keywords

allelopathy / compositae plants / Microcystis aeruginosa / inhibition rate / logistic model analysis

References

[1] AndersonD M. Toxic algal blooms and red tides: A global perspective. In: Okaichi T, Anderson D M, Nemoto T, eds. Red Tides: Biology Environmental Science and Toxicology. New York: Elsevier, 1989, 11–16

[2] SmaydaT J. Primary production and the global epidemic of phytoplankton blooms in the sea: A linkage? In: Cosper E M, Bricelj V M, Carpenter E J, eds. Novel Phytoplankton Blooms, Coastal and Estuarine Studies Number 35. New York: Springer-Verlag, 1989: 449–484

[3] SmaydaT J. Novel and nuisance phytoplankton blooms in the sea: Evidence for a global epidemic. In: Graneli E, ed. Toxic Marine Phytoplankton: Fourth International Conference, Lund, Sweden, June 26–30, Vol 21. New York: Elsevier Science, 1989: 29–40

[4] HallegraeffG M. A review of harmful algal blooms and their apparent global increase. Phycologia, 32: 79–99

[5] Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB). Global Ecology and Oceanography of Harmful Algal Blooms, Science Plan. In: Gilbert P, Pitcher G., eds. Scientific Committee on Oceanic Research (SCOR) and Intergovernmental Oceanographic Commission (IOC), Baltimore and Paris , 2001: 86

[6] ZingoneA, EnevoldsenH O. The diversity of harmful algal blooms: A challenge for science and management. Ocean and Coastal Management, 2000, 43: 725–748

[7] SunX X, ChoiJ K, KimE K. A preliminary study on the mechanism of harmful algal bloom mitigation by use of sophorolipid treatment. Journal of Experimental Marine Biology and Ecology, 2004, 304: 35–49

[8] RiceE L. Allelopathy. 2nd.Orlando: Academic Press, 1984, 12

[9] NarwalS S. Allelopathy in ecological agriculture. Allelopathy in Ecological Agriculture and Forest2000: 11–32

[10] HuH Y. Research progress on phyto-allelopathic algae control. Ecology and Environment, 2006, 15(1): 153–157 (in Chinese)

[11] ZhuangY Y, ZhaoF, DaiS G. Algal growth inhibition by phytotoxins. Advances in Environmental Science, 1995, 6(3): 44–49 (in Chinese)

[12] ZhangW H, ZhouL F, WuX G, SongL R. Allelopathic effect of Acorus calamus on Microcystis aeruginosa. China Environmental Science, 2006, 26(3): 355–358 (in Chinese)

[13] ZhanY L, LuP, WuX F. A Review of researches on phyto-allelopathy in algal inhibition. Environmental Science and Management, 2006, 7(31): 50–52

[14] LiY J, JinX C, NianY G, HuS R, HuX Z. Artificial floating island technology and its applied research. Technology of Water Treatment, 2007, 33(10): 49–51 (in Chinese)

[15] ZhouK. The research progress of compositae on allelopathy. Acta Ecological Sinica, 2004, 24(8): 1780–1788 (in Chinese)

[16] LiF H. The History and status in soilless culture advances. Heilonjiang Institute of Agricultural Modernization Chinese Academy of Sciences, 1999, 15(4): 313–514 (in Chinese)

[17] SavvasD, ManosG. Automated composition control of nutrient solution in closed soilless culture systems. Journal of Agricultura Engineering Research, 1999, 73(1): 29–33

[18] A1tunluH. Postharvest quality of cucumber grown by soilless culture. Acta Horticulture, 2000, 517: 287–292

[19] BohmeM, OuahidA, ShabanN. Reaction of some vegetable crops to treatments with lactate as bioregulator and fertilizer. Acta Horticulture, 2000, 514(P4): 33–40

[20] SongL, LiuY, GanX, ZhuY, YuM. FACHB-collection: General introduction and the strain list. Acta Hydrobiologica Sinica, 1999, 23(Suppl) 537–546

[21] ZhangX, HuH Y, MemY J. Inhibitory effect of extract from barley straw on the growth of Microcystis aeruginosa. Acta Scientiae Circumstantiae, 2007, 27(12): 1984–1987 (in Chinese)

[22] DingH J, PengQ, ZhangW H, WuX G, ZhouL F, FangT. Inhibition Effect of wetland plants on Microcystis aeruginosa. China Environmental Science, 2006, 26(3): 355–358 (in Chinese)

[23] HuX W, DongY Y, ZhangX P, YeF B. The measurement of anabaena flos-aquae with visible spectrophotography. Journal of Huazhong Agricultural, 2002, 21(3): 296–297 (in Chinese)

[24] SchanzF, ZahlerU. Prediction of algal growth in batch cultures. Aquatic Sciences-Research Across Boundaries, 1981, 43(1): 103–113

[25] ParkM H, HanM S, AhnC Y, KimH S, YooB D, OhH M. Growth inhibition of bloom-forming cyanobacterium Microcystis aeruginosa by rice straw extract. The Society for Applied Microbiology, Letters in Applied Microbiology, 2006, 43: 307–312

[26] ChenS Y, XiongD Q. The fuzzy set theory and model for assessing lake eutrophication. Journal of Lake Science, 1993, 5(2): 144–152 (in Chinese)

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 20877060), and the Project of the State Key Laboratory of Freshwater Ecology and Biotechnology (Grant No. 2005 FB06). The authors would like to thank School of Resource and Enviormental Science, Wuhan University for its finical support as well (Water Environment Research & Data Sharing Platform in the Middle Reaches of the Yangtse River, Grant No. WERDSPMYR-0606).

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg