Performance of completely autotrophic nitrogen removal over nitrite process under different aeration modes and dissolved oxygen

Abstract

In this study, three sequential batch biofilm reactors (SBBRs) were operated for 155 days to evaluate the performance of completely autotrophic nitrogen removal over nitrite (CANON) process under different aeration modes and dissolved oxygen (DO). Synthetic wastewater with 160-mg NH₄⁺-N/L was fed into the reactors. In the continuously-aerated reactor, the efficiency of the ammonium nitrogen conversion and total nitrogen (TN) removal reached 80% and 70%, respectively, with DO between 0.8–1.0 mg/L. Whereas in the intermittently-aerated reactor, at the aeration/non-aeration ratio of 1.0, ammonium was always under the detection limit and 86% of TN was removed with DO between 2.0–2.5 mg/L during the aeration time. Results show that CANON could be achieved in both continuous and intermittent aeration pattern. However, to achieve the same nitrogen removal efficiency, the DO needed in the intermittently-aerated sequential batch biofilm reactor (SBBR) during the aeration period was higher than that in the continuously-aerated SBBR. In addition, the DO in the CANON system should be adjusted to the aeration mode, and low DO was not a prerequisite to CANON process.

References

1. Chen S K, Juaw C K, Chen S S . Nitrification and denitrification of high-strength ammoniumand nitrite wastewater with biofilm reactors. Water Science and Technology, 1991, 23: 1417–1425
2. Yoo H, Ahn K H, Lee H J, Kwak Y J, Song K G . Nitrogen removal from synthetic wastewaterby simultaneous nitrification denitrification (SND) via nitrite inan intermittently-aerated reactor. WaterResearch, 1999, 33(1): 145–154. doi:10.1016/S0043-1354(98)00159-6
3. Jetten M S M, Horn H J, van Loosdrecht M C M . Towards a more sustainable municipalwastewater treatment system. Water Scienceand Technology, 1997, 35(9): 171–180. doi:10.1016/S0273-1223(97)00195-9
4. Strous M, van Gerven E, Zheng P, Kuenen J G, Jetten M S M . Ammonium removal from concentrated wastestreams with the anaerobic ammonium oxidation (ANAMMOX) process indifferent configurations. Water Research, 1997, 31(8): 1955–1962. doi:10.1016/S0043-1354(97)00055-9
5. Graff A A, Mulder A, de Bruijn P, Jetten M S, Robertson L A, Kuenen J G . Anaerobic oxidation of ammonium is a biologically mediatedprocess. Applied & Environmental Microbiology, 1995, 61(4): 1246–1251
6. Kuai L, Verstraete W . Ammonium removal by the oxygen-limitedautotrophic nitrification-denitrification system. Applied & Environmental Microbiology, 1998, 64(11): 4500–4506
7. Pynaert K, Smets B F, Wyffels S, Beheydt D, Siciliano S D, Verstraete W . Characterization of an autotrophic nitrogen-removingbiofilm from a highly loaded labscale rotating biological contactor. Applied & Environmental Microbiology, 2003, 69(6): 3626–3635. doi:10.1128/AEM.69.6.3626-3635.2003
8. Strous M . Microbiologyof anaerobic ammonium oxidation. Dissertation for the Doctoral Degree. Delft, the Netherlands:Delft University of Technology, 2000, 68–81
9. Sliekers A O, Third K A, Abma W, Kuenen J G, Jetten M S . CANON and anammox in a gas-lift reactor. FEMS Microbiology Letters, 2005, 218(2): 339–344. doi:10.1016/S0378-1097(02)01177-1
10. Hippen A, Rosenwinkel K H, Baumgarten G, Seyfried C F . Aerobic deammonification: A new experience in the treatment of wastewaters. Water Science and Technology, 1997, 35(10): 111–120. doi:10.1016/S0273-1223(97)00211-4
11. Rosenwinkel K H, Cornelius A . Deammonification in the moving-bedprocess for the treatment of wastewater with high ammonia content. Chemical Engineering & Technology, 2005, 28(1): 49–52. doi:10.1002/ceat.200407070
12. Hao X D, Heijnen J J, van Loosdrecht M C M . Model-based evaluation of temperatureand inflow variations on a partial nitrification-ANAMMOX biofilm process. Water Research, 2002, 36(19): 4839–4849. doi:10.1016/S0043-1354(02)00219-1
13. Jetten M S M, Strous M, van de Pas-Schoonen K T, Schalk J, van Dongen U G, van de Graaf A A, Logemann S, Kuenen J G . The anaerobic oxidation ofammonium. FEMS Microbiology Reviews, 1998, 22(5): 421–437. doi:10.1111/j.1574-6976.1998.tb00379.x
14. Hao X D, Heijnen J J, van Loosdrecht M C M . Sensitivity analysis of a biofilm modeldescribing a one-stage completely autotrophic nitrogen removal (CANON)process. Biotechnology and Bioengineering, 2002, 77(3): 266–277. doi:10.1002/bit.10105
15. Nielsen M, Bollmann A, Sliekers O, Jetten M, Strous M, Schmidt I, Larsen L H, Nielsen L P, Revsbech N P . Kinetics, diffusional limitation and microscale distribution of chemistryand organisms in a CANON reactor. FEMSMicrobiology Ecology, 2005, 51(2): 247–256. doi:10.1016/j.femsec.2004.09.003
16. Gong Z, Liu S T, Yang F L, et al.. Characterization of functional microbial communityin a membrane-aerated biofilm reactor operated for completely autotrophicnitrogen removal. Bioresource Technology, 2008, 99(8): 2749–2756. doi:10.1016/j.biortech.2007.06.040
17. Helmer C, Tromm C, Hippen A, Rosenwinkel K H, Serfried C F, Kunst S . Single stage biological nitrogen removal by nitrificationand anaerobic ammonium oxidation in biofilm systems. Water Science and Technology, 2001, 43(1): 311–320
18. Liao D X, Li X M, Zeng G M, Yang Q, Yang G J . Studies on deammonification in singleSBR biofilm. China Environmental Science, 2005, 25(2): 222–225 (in Chinese)
19. Kaam R V, Anne Archard D, Alliet M, Lopez S, Albast C . Aeration mode, shear stress and sludgerheology in a submerged membrane bioreactor: Some keys of energy saving. Desalination, 2006, 199(1–3): 482–484
20. You K, Fu J X, Ju R . Experimental studies on the effect of intermittence aerationon membrane bioreactor. Industrial Safetyand Environmental Protection, 2006, 32(7): 16–18 (in Chinese)
21. Pollice A, Tandoi V, Lestingi C . Influence of aeration and sludge retention time on ammoniumoxidation to nitrite and nitrate. WaterResearch, 2002, 36(10): 2541–2546. doi:10.1016/S0043-1354(01)00468-7
22. Zhang H, Bai X Y, Li J, Lu P . Study on anew way of shortcut nitrification with intermittent aeration. Environmental Protection of Xinjiang, 2005, 27(4): 28–32 (in Chinese)
23. Guo J S, Fang F, Luo B F, Qin Y, Yang G H . Start-up experiment on nitrificationof mid-low ammonium concentration wastewater in SBR biofilm reactor. Environmental Science, 2006, 27(4): 686–690 (in Chinese)
24. State EnvironmentalProtection Administration of China. . Methods of Monitoring Waterand Wastewater in Chinese. 4th ed. Beijing: Chinese EnvironmentalScience Press, 2002 (in Chinese)
25. Third K A, Sliekers A O, Kuenen J G, Jetten M S M . The CANON(completely autotrophic nitrogen-removal over nitrite) under ammoniumlimitation: Interaction and competition between three groups of bacteria. Systematic and Applied Microbiology, 2001, 24(4): 588–596. doi:10.1078/0723-2020-00077
26. Zheng P, Hu B L, Xu X Y . Aerobic metabolism of mixed culture of ammonium oxidation. Journal of Zhejiang University (Agriculture &Life Science), 2000, 26(5): 521–526 (in Chinese)
27. Wyffels S, Boeckx K, Zhang D, van Cleemput O, Chen G, Verstraete W . Nitrogen removal from sludge reject water by a two-stageoxygen-limited autotrophic nitrification denitrification process. Water Science and Technology, 2004, 49(5–6): 57–64