Detection of Escherichia coli in wastewater based on enzyme immunoassay

Abstract

This research describes a fast detection method on the basis of enzyme-linked immunosorbent assay (ELISA) for Escherichia coli in drainage of wastewater treatment plants. Optimized conditions such as the reaction format (sandwich or direct), the concentrations of diluted horse radish peroxidase (HRP)-E. coli conjugate, and anti-HPR antibody and pretreatment of E. coli were studied. Those results showed that the linear range of detection for E. coli was 10 cfu/mL– 6 × 10⁴ cfu/mL. Compared with conventional methods, it is a convenient and sensitive detection method with low cost.

Keywords

Escherichia coli, fast detection, enzyme-linked immunosorbent assay (ELISA), wastewater treatment plant