Frontiers of Chemical Science and Engineering >

2010 , Vol. 4 >Issue 1: 96 - 101

DOI: https://doi.org/10.1007/s11705-009-0309-z

Research articles

LC-ELSD analysis for simultaneous determination of five components in the Xue-Fu-Zhu-Yu capsules

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  • 1.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China；Medical College of Chinese People''s Armed Police Forces, Tianjin 300162, China 3. Tianjin Hongrentang Pharmaceutical Co., Ltd., Tianjin 300122, China; 2.School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China; 3. ;

Published date: 05 Mar 2010

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Abstract

A reliable and accurate liquid chromatography with evaporative light scattering detection (LC-ELSD) method was developed for simultaneous determination of five constituents in Xue-Fu-Zhu-Yu capsule, a widely used prescription of traditional Chinese medicine (TCM). The chromatographic separation was performed on a Symmetry C18 column (150mm×4.6mm, 5.0μm) using gradient elution of acetonitrile and water (containing 1.0% acetic acid). The flow rate was 0.6mL/min and the temperature of column was 30°C. A good linearity correlation was obtained over the investigated concentration ranges. Recoveries of the five components varied from 97.31% to 99.84% (RSD<4.0%). The validated method was successfully applied to the quality assessment of Xue-Fu-Zhu-Yu capsule.

Cite this article

Ying GAO, Wenyuan GAO, Xuan DONG, Fengge LI, Xinying YANG, . LC-ELSD analysis for simultaneous determination of five components in the Xue-Fu-Zhu-Yu capsules[J]. Frontiers of Chemical Science and Engineering, 2010 , 4(1) : 96 -101 . DOI: 10.1007/s11705-009-0309-z

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