Erythromycin biosynthesis is a highly complicated process, which involves both primary metabolism and secondary metabolism. The specific activities of the key enzymes related to glucose metabolism such as hexose kinase (HK), glucose-6-phosphate dehydrogenase (6-PDH), phosphofructokinase (PFK), and isocitrate dehydrogenase (ICD), were determined in Saccharopolyspora erythraea. The specific activities of the enzymes involved in secondary metabolism, such as methylmalonyl-coenzyme A mutase (MCM) and methylmalonyl-coenzyme A transcarboxylase (MCT), were detected as well. Some organic acids contained in fermentation broth were also analyzed. The results show that Co2+ is able to increase erythromycin biosynthesis. It maybe due to Co2+ improving the specific activities of methylmalonyl-coenzyme A mutase and methylmalonyl-coenzyme A transcarboxylase. Meanwhile, it also enhances the flux of the glucose metabolism pathway.
DU Wen, CHEN Changhua
. Effects of Co2+ on the erythromycin biosynthesis[J]. Frontiers of Chemical Science and Engineering, 2007
, 1(3)
: 246
-250
.
DOI: 10.1007/s11705-007-0044-2