Frontiers of Chemical Science and Engineering >
Mercury removal and recovery by immobilized Bacillus megaterium MB1
Received date: 06 Nov 2011
Accepted date: 20 Feb 2012
Published date: 05 Jun 2012
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From several mercury removing microorganisms, we selected Bacillus megaterium MB1, which is non-pathogenic, broad-spectrum mercury resistant, mercuric ion reducing, heat tolerant, and spore-forming, as a useful bacterium for bioremediation of mercury pollution. In this study, mercury removal performance of the immobilized B. megaterium MB1 was investigated to develop safe, efficient and stable catalytic bio-agent for mercury bioremediation. The results showed that the alginate gel immobilized B. megaterium MB1 cells efficiently removed 80% of mercury from the solution containing 10 mg/L mercuric chloride within 24 h. These cells still had high activity of mercury removal even after mercuric ion loading was repeated for nine times. The analysis of mercury contents of the alginate beads with and without immobilized B. megaterium MB1 suggested that a large portion of reduced metallic mercury was trapped in the gel beads. It was concluded that the alginate gel immobilized B. megaterium MB1 cells have potential to remove and recover mercury from mercury-containing water.
Key words： mercury removal; immobilized bacteria; alginate gel; bioremediation
Meifang CHIEN , Ryo NAKAHATA , Tetsuya ONO , Keisuke MIYAUCHI , Ginro ENDO . Mercury removal and recovery by immobilized Bacillus megaterium MB1[J]. Frontiers of Chemical Science and Engineering, 2012 , 6(2) : 192 -197 . DOI: 10.1007/s11705-012-1284-3
| 1 |
Harada M. Minamata disease: methylmercury poisoning in Japan caused by environmental pollution. Critical Reviews in Toxicology, 1995, 25(1): 1-24
|
| 2 |
Barkay T, Miller S M, Summers A O. Bacterial mercury resistance from atoms to ecosystems. FEMS Microbiology Reviews, 2003, 27(2-3): 355-384
|
| 3 |
Osborn A M, Bruce K D, Strike P, Ritchie D A. Distribution, diversity and evolution of the bacterial mercury resistance (mer) operon. FEMS Microbiology Reviews, 1997, 19(4): 239-262
|
| 4 |
Bogdanova E S, Bass I A, Minakhin L S, Petrova M A, Mindlin S Z, Volodin A A, Kalyaeva E S, Tiedje J M, Hobman J L, Brown N L, Nikiforov V G. Horizontal spread of mer operons among gram-positive bacteria in natural environments. Microbiology, 1998, 144(3): 609-620
|
| 5 |
Laddaga R A, Chu L, Misra T K, Silver S. Nucleotide sequence and expression of the mercurial-resistance operon from Staphylococcus aureus plasmid pI258. Proceedings of the National Academy of Sciences of the United States of America, 1987, 84(15): 5106-5110
|
| 6 |
Kiyono M, Omura T, Inuzuka M, Fujimori H, Pan-Hou H. Nucleotide sequence and expression of the organomercurial-resistance determinants from a Pseudomonas K-62 plasmid pMR26. Gene, 1997, 189(2): 151-157
|
| 7 |
Wang Y, Moore M, Levinson H S, Silver S, Walsh C, Mahler I. Nucleotide sequence of a chromosomal mercury resistance determinant from a Bacillus sp. with broad-spectrum mercury resistance. Journal of Bacteriology, 1989, 171(1): 83-92
|
| 8 |
Huang C C, Narita M, Yamagata T, Itoh Y, Endo G. Structure analysis of a class II transposon encoding the mercury resistance of the Gram-positive Bacterium bacillus megaterium MB1, a strain isolated from minamata bay, Japan. Gene, 1999, 234(2): 361-369
|
| 9 |
Narita M, Chiba K, Nishizawa H, Ishii H, Huang C C, Kawabata Z, Silver S, Endo G. Diversity of mercury resistance determinants among Bacillus strains isolated from sediment of Minamata Bay. FEMS Microbiology Letters, 2003, 223(1): 73-82
|
| 10 |
Chen Y M, Lin T F, Huang C, Lin J C, Hsieh F M. Degradation of phenol and TCE using suspended and chitosan-bead immobilized Pseudomonas putida. Journal of Hazardous Materials, 2007, 148(3): 660-670
|
| 11 |
Wang X, Gai Z, Yu B, Feng J, Xu C, Yuan Y, Lin Z, Xu P. Degradation of carbazole by microbial cells immobilized in magnetic gellan gum gel beads. Applied and Environmental Microbiology, 2007, 73(20): 6421-6428
|
| 12 |
Okino S, Iwasaki K, Yagi O, Tanaka H. Removal of mercuric chloride by immobilized cells of genetically modified Pseudomonas putida PpY101/pSR134. Journal of Environmental Biotechnology, 2001, 1: 41-47
|
| 13 |
Chien M F, Narita M, Lin K H, Matsui K, Huang C C, Endo G. Organomercurials removal by heterogeneous merB genes harboring bacterial strains. Journal of Bioscience and Bioengineering, 2010, 110(1): 94-98
|
| 14 |
Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd ed. New York: Cold Spring Harbor Laboratory, Cold Spring Harbor, 1989, A2. 2
|
| 15 |
Sinha A, Khare S K. Mercury bioremediation by mercury accumulating Enterobacter sp. cells and its alginate immobilized application. Biodegradation, 2012, 23(1): 25-34
|
| 16 |
Kiyono M, Omura H, Omura T, Murata S, Pan-Hou H. Removal of inorganic and organic mercurials by immobilized bacteria having mer-ppk fusion plasmids. Applied Microbiology and Biotechnology, 2003, 62(2-3): 274-278
|
| 17 |
Harel P, Mingot L, Junter G A. Cadmium removal from dilute aqueous solution by beads of polysaccharide gels usable for microbial cell immobilization. International Biodeterioration & Biodegradation, 1996, 37(3-4): 239-240
|
| 18 |
Lázaro N, Sevilla A L, Morales S, Marqués A M. Heavy metal biosorption by gellan gum gel beads. Water Research, 2003, 37(9): 2118-2126
|
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