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Efficient production of D-1,2,4-butanetriol from D-xylose by engineered Escherichia coli whole-cell biocatalysts
Received date: 03 Feb 2018
Accepted date: 06 Apr 2018
Published date: 03 Jan 2019
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We have developed a whole-cell bioconversion system for the production of D-1,2,4-butanetriol (BT) from renewable biomass. A plasmid pETduet-xylB-yjhG-T7-adhP-T7-mdlC was constructed and transformed to Escherichia coli BL21(DE3) to obtain the whole cells of E. coli BL21-XYMA capable of bioconversion D-xylose to BT. Then, the factors including carbon sources, nitrogen sources, metal ions, and culture conditions (pH, temperature, IPTG) were identified, and their effects on the whole-cell activity for BT production were investigated. To obtain the highest whole-cell activity, the optimal cultivation parameters are: 15 g·L−1 yeast extract, 5 g·L−1 sucrose, 3 g·L−1 KH2PO4, 5 g·L−1 NaCl, 3 g·L−1 NH4Cl, 0.25 g·L−1 MgSO4∙7H2O and 1 mL·L−1 the mixture of trace elements. With the optimized whole cells of E. coli BL21-XYMA, 60 g·L−1 of xylose was converted to 28 g·L−1 BT with a molar yield of 66.0%, which is higher than those reported in the biotechnological system.
Shewei Hu , Qian Gao , Xin Wang , Jianming Yang , Nana Xu , Kequan Chen , Sheng Xu , Pingkai Ouyang . Efficient production of D-1,2,4-butanetriol from D-xylose by engineered Escherichia coli whole-cell biocatalysts[J]. Frontiers of Chemical Science and Engineering, 2018 , 12(4) : 772 -779 . DOI: 10.1007/s11705-018-1731-x
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