Purification and concentration of gluconic acid from an integrated fermentation and membrane process using response surface optimized conditions

Abstract

A response surface method was used to optimize the purification and concentration of gluconic acid from fermentation broth using an integrated membrane system. Gluconobacter oxydans was used for the bioconversion of the glucose in sugarcane juice to gluconic acid (concentration 45 g∙L⁻¹) with a yield of 0.9 g∙g⁻¹. The optimum operating conditions, such as trans-membrane pressure (TMP), pH, cross-flow rate (CFR) and initial gluconic acid concentration, were determined using response surface methodology. Five different types of polyamide nanofiltration membranes were screened and the best performing one was then used for downstream purification of gluconic acid in a flat sheet cross-flow membrane module. Under the optimum conditions (TMP= 12 bar and CFR= 400 L∙h⁻¹), this membrane retained more than 85% of the unconverted glucose from the fermentation broth and had a gluconic acid permeation rate of 88% with a flux of 161 L∙m⁻²∙h⁻¹. Using response surface methods to optimize this green nanofiltration process is an effective way of controlling the production of gluconic acid so that an efficient separation with high flux is obtained.

Key words： gluconic acid; optimized nanofiltration; green processing; process intensification

Acknowledgements

The authors thank the Department of Science and Technology, Government of India (DST) for the grants under the DST-FIST Program and the University Grant Commission, Government of India for financial assistance under the Dr. D.S. Kothari Post-Doctoral Fellowship (No.F.4-2/2006 (BSR)/EN/16-17/ 0001).

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