Biodegradation and detoxification of the triphenylmethane dye coomassie brilliant blue by the extracellular enzymes from mycelia of Lactarius deliciosus

Abstract

Fungi play an important role in dying wastewater treatment. In this work, the mycelia of Lactarius deliciosus exhibited an excellent capacity in decolorizing coomassie brilliant blue (CBB). The results demonstrated that the mycelia could treat CBB with high concentrations over a broad range of pH and temperature. The decolorization rate of 99.19% and the removal rate of 16.31 mg·L^‒1·h were realized. The mycelia could be recycled from decolorizing process for 19 times, indicating a good re-usability. It verified that the lignin peroxidase (121.65 U·L^‒1) and manganese peroxidase (36.77 U·L^‒1) were involved in the degradation and decolorization process of CBB. Toxicity assessments indicated the seed germination rate was up to 82.22% while inhibition to Escherichia coli decreased dramatically and no significant effect on Caenorhabditis elegans growth was found. The removal of CBB was a synergistic process accomplished by adsorption and biodegradation. The mycelia could be used for eco-friendly CBB treatment.

Graphical abstract

Keywords

fungus mycelia / biodegradation / extracellular enzymes / coomassie brilliant blue / Lactarius deliciosus

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Acknowledgments

This work was supported by the Anhui Provincial Program on Key Research and Development Project (Grant No. 202004a06020021), the National Natural Science Foundation of China (Grant No. 21606002), the Natural Science Foundation of Anhui Province (CN) (Grant No. 1708085QC64) and the Undergraduate Research Training Programs for Innovation (Grant Nos. 201910357069, S201910357427).

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11705-020-1952-7 and is accessible for authorized users.

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