A cytoprotective graphene oxide-polyelectrolytes nanoshell for single-cell encapsulation

Abstract

Graphene oxide (GO) has been increasingly utilized in the fields of food, biomedicine, environment and other fields because of its benign biocompatible. We encapsulated two kinds of GO with different sizes on yeast cells with the assistance of polyelectrolytes poly (styrene sulfonic acid) sodium salt (PSS) and polyglutamic acid (PGA) (termed as Y@GO). The result does not show a significant difference between the properties of the two types of Y@GO (namely Y@GO1 and Y@GO2). The encapsulation layers are optimized as Yeast/PGA/PSS/PGA/GO/PGA/PSS based on the morphology, dispersity, colony-forming unit, and zeta potential. The encapsulation of GO increases the roughness of the yeast. It is proved that the Y@GO increases the survival time and enhance the activity of yeast cells. The GO shell improves the resistance of yeast cells against pH and salt stresses and extends the storage time of yeast cells.

Graphical abstract

Keywords

GO / yeast / polyelectrolyte / cytoprotection / nanomaterials

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Acknowledgements

This work is supported by the National Key Research and Development Project of China (Grant No. 218YFA0903000), the National Natural Science Foundation of China (Grant Nos. 21606013, 21301015), National Mega-project for Innovative Drugs (Grant No. 2019ZX09721001-007-002), and Shenzhen Science and Technology Project (Grant No. JCYJ20180507183842516).

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2020 Higher Education Press