Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
Juan Wang , Bin Jia , Zexiong Xie , Yunxiang Li , Yingjin Yuan
Front. Chem. Sci. Eng. ›› 2018, Vol. 12 ›› Issue (4) : 806 -814.
Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles
The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.
synthetic biology / genome rearrangement / prodeoxyviolacein / SCRaMbLE / Saccharomyces cerevisiae
Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature
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