Improving prodeoxyviolacein production via multiplex SCRaMbLE iterative cycles

Abstract

The synthetic chromosome rearrangement and modification by loxP-mediated evolution (SCRaMbLE) system has been used to improve prodeoxyviolacein (PDV) production in haploid yeast containing chromosome synV. To rapidly and continuously generate genome diversification with the desired phenotype, the multiplex SCRaMbLE iterative cycle strategy has been developed for the screening of high PDV production strains. Whole-genome sequencing analysis reveals large duplications, deletions, and even the whole genome duplications. The deletion of YER151C is proved to be responsible for the increase. This study demonstrates that artificial DNA rearrangement can be used to accelerate microbial evolution and the production of biobased chemicals.

Graphical abstract

Keywords

synthetic biology / genome rearrangement / prodeoxyviolacein / SCRaMbLE / Saccharomyces cerevisiae

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Acknowledgements

We thank the National Program on Key Basic Research Project of China (2014CB745100) and the National Natural Science Foundation of China (21750001 and 21621004) for funding.

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11705–018-1739-2 and is accessible for authorized users.

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