Detoxification and concentration of corn stover hydrolysate and its fermentation for ethanol production

Abstract

Environmental and energy concerns have increased interest in renewable energy sources, particularly biofuels. Thus the fermentation of glucose from sulfuric acid-hydrolyzed corn stover for the production of bioethanol has been explored using a combined acid retardation and continuous-effect membrane distillation treatment process. This process resulted in the separation of the sugars and acids from the acid-catalyzed hydrolysate, the removal of most of the fermentation inhibitors from the hydrolysate and the concentration of the detoxified hydrolysate. The recovery rate of glucose from the sugar-acid mixture using acid retardation was greater than 99.12% and the sulfuric acid was completely recovered from the hydrolysate. When the treated corn stover hydrolysate, containing 100 g/L glucose, was used as a carbon source, 43.06 g/L of ethanol was produced with a productivity of 1.79 g/(L∙h) and a yield of 86.31%. In the control experiment, where glucose was used as the carbon source these values were 1.97 g/(L∙h) and 93.10% respectively. Thus the integration of acid retardation and a continuous-effect membrane distillation process are effective for the production of fuel ethanol from corn stover.

Graphical abstract

Keywords

corn stover / hydrolysate / acid retardation / continuous-effect membrane distillation / ethanol fermentation

References

[1] Kim I, Seo Y H, Kim G Y, Han J I. Co-production of bioethanol and biodiesel from corn stover pretreated with nitric acid. Fuel, 2015, 143: 285–289

[2] Koppram R, Nielsen F, Albers E, Lambert A, Waennstroem S, Welin L, Zacchi G, Olsson L. Simultaneous saccharification and co-fermentation for bioethanol production using corncobs at lab, PDU and demo scales. Biotechnology for Biofuels, 2013, 6(1): 2

[3] Karin O, Andreas R, Mats G, Guido Z. Fuel ethanol production from steam-pretreated corn stover using SSF at higher dry matter content. Biomass and Bioenergy, 2006, 30(10): 863–869

[4] Zaldivar J, Nielsen J, Olsson L. Fuel ethanol production from lignocellulose: A challenge for metabolic engineering and process integration. Applied Microbiology and Biotechnology, 2001, 56(1-2): 17–34

[5] Taylor M P, Mulako I, Tuffin M, Cowan D. Understanding physiological responses to pretreatment inhibitors in ethanologenic fermentations. Journal of Biotechnology, 2012, 7(9): 1169–1181

[6] Mingjie J, Ming W L, Venkatesh B, Bruce E D. Two-step SSCF to convert AFEX-treated switchgrass to ethanol using commercial enzymes and Saccharomyces cerevisiae 424A(LNH-ST). Bioresource Technology, 2010, 101(21): 8171–8178

[7] Yomano L P, York S W, Shanmugam K T, Ingram L O. Deletion of methylglyoxal synthase gene (mgsA) increased sugar co-metabolism in ethanol-producing Escherichia coli. Biotechnology Letters, 2009, 31(9): 1389–1398

[8] Huang R, Su R. Qi W, Zhang M, He Z. Fractionation of lignocellulose by formic acid pretreatment. Chinese Journal of Process Engineering, 2008, 8(6): 1103–1107

[9] Heinonen J, Tamminen A, Uusitalo J, Sainio T. Ethanol production from wood via concentrated acid hydrolysis, chromatographic separation, and fermentation. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 2012, 87(5): 689–696

[10] Moe S T, Janga K K, Hertzberg T, Hagg M B, Oeyaas K, Dyrset N. Saccharification of lignocellulosic biomass for biofuel and biorefinery applications—a renaissance for the concentrated acid hydrolysis? Energy Procedia, 2012, 20: 50–58

[11] Wang L, Chen H. Increased fermentability of enzymatically hydrolyzed steam-exploded corn stover for butanol production by removal of fermentation inhibitors. Process Biochemistry, 2011, 46(2): 604–607

[12] Martinez A, Rodriguez M E, Wells M L, York S W, Preston J F, Ingram L O. Detoxification of dilute acid hydrolysates of lignocellulose with lime. Biotechnology Progress, 2001, 17(2): 287–293

[13] Sainio T, Turku I, Heinonen J. Adsorptive removal of fermentation inhibitors from concentrated acid hydrolyzates of lignocellulosic biomass. Bioresource Technology, 2011, 102(10): 6048–6057

[14] Cho D H, Lee Y J, Um Y, Sang B I, Kim Y H. Detoxification of model phenolic compounds in lignocellulosic hydrolysates with peroxidase for butanol production from Clostridium beijerinckii. Applied Microbiology and Biotechnology, 2009, 83(6): 1035–1043

[15] Purwadi R, Niklasson C, Taherzadeh M J. Kinetic study of detoxification of dilute-acid hydrolyzates by Ca(OH)₂. Journal of Biotechnology, 2004, 114(1-2): 187–198

[16] Nanguneri S R, Hester R D. Acid/sugar separation using ion exclusion resins: A process analysis and design. Separation Science and Technology, 1990, 25(13-15): 1829–1842

[17] Neuman R P, Rudge S R, Ladisch M R. Sulfuric acid-sugar separation by ion exclusion. Reactive Polymers, Ion Exchangers. Sorbents, 1987, 5(1): 55–61

[18] Hatch M J, Dillon J A. Acid retardation: Simple physical method for separation of strong acids from their salts. Industrial & Engineering Chemistry Process Design and Development, 1963, 2(4): 253–263

[19] Liu J, Qin Y, Li P, Zhang K, Liu Q, Liu L. Separation of the acid-sugar mixtures by using acid retardation and further concentration of the eluents by using continuous-effect membrane distillation. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 2016, 91(4): 1105–1112

[20] Chen J, Zhang Y, Wang Y, Ji X, Zhang L, Mi X, Huang H. Removal of inhibitors from lignocellulosic hydrolyzates by vacuum membrane distillation. Bioresource Technology, 2013, 144: 680–683

[21] Yao K, Qin Y, Yuan Y, Liu L, He F, Wu Y. A continuous-effect membrane distillation process based on hollow fiber AGMD module with internal latent-heat recovery. AIChE Journal, 2013, 59(4): 1278–1297

[22] Yang W, Li P, Bo D, Chang H, Wang X, Zhu T. Optimization of furfural production from D-xylose with formic acid as catalyst in a reactive extraction system. Bioresource Technology, 2013, 133: 361–369

[23] Zhang K, Qin Y, He F, Liu J, Zhang Y, Liu L. Concentration of aqueous glycerol solution by using continuous-effect membrane distillation. Separation and Purification Technology, 2015, 144: 186–196

[24] Liu J. The research of key points on biobutanol production using corn stover. Dissertation for the Doctoral Degree. Tianjin: Tianjin University, 2015, 64–76

[25] Seidel-Morgenstern A, Schulte M, Epping A. Fundamentals and General Terminology, in Preparative Chromatography. Weinheim: Wiley-VCH Verlag, 2012, 7–46

[26] Clark T A, Mackie K L. Fermentation inhibitors in wood hydrolysates derived from the softwood Pinus radiata. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), 1984, 34B(2): 101–110

[27] Grassmanned B P, Sawisto E B H. Physical principles of chemical engineering. Oxford: Pergamon Press, 1971

[28] Almeida e Silva J B, Lima U A, Taqueda M E S, Guaragna F G. Use of response surface methodology for selection of nutrient levels for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolyzate. Bioresource Technology, 2003, 87(1): 45–50

[29] Yue G J. An introduction to cellulosic ethanol engineering. Beijing: Chemical Industry Press, 2014, 5–29 (in Chinese)

[30] Sierkstra L N, Silljé H H W, Verbakel J M A, Verrips C T. The glucose-6 phosphate isomerase reaction is essential for normal glucose repression in Saccharomyces cerevisiae. FEBS Journal, 1993, 214(1): 121–127

[31] Maiorella B, Blanch H W, Wilke C R. By-product inhibition effects on ethanolic fermentation by Saccharomyces cerevisiae. Biotechnology and Bioengineering, 1983, 25(1): 103–121

[32] Torija M J, Rozes N, Poblet M, Guillamon J M, Mas A. Effects of fermentation temperature on the strain population of Saccharomyces cerevisiae. International Journal of Food Microbiology, 2003, 80(1): 47–53

[33] Phisalaphong M, Srirattana N, Tanthapanichakoon W. Mathematical modeling to investigate temperature effect on kinetic parameters of ethanol fermentation. Biochemical Engineering Journal, 2006, 28(1): 36–43

[34] Zhang K C. Alcohol and Distilling Wine Craft. Beijing: China Light Industry Press, 1995, 246–247 (in Chinese)

[35] Cai L Y, Ma Y L, Ma X X, Lv J M. Improvement of enzymatic hydrolysis and ethanol production from corn stalk by alkali and N-methylmorpholine-N-oxide pretreatments. Bioresource Technology, 2016, 212: 42–46

[36] Messaoudi Y, Smichi N, Bouachir F, Gargouri M. Fractionation and biotransformation of lignocelluloses-based wastes for bioethanol, xylose and vanillin production. Waste and Biomass Valorization, 2017, DOI: https://doi.org/10.1007/s12649-017-0062-3

Acknowledgments

The authors gratefully acknowledge the financial support of the National Natural Science Foundation of China (Grant No. 21376175).

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