Streptomyces ghanaensis VITHM1 mediated green synthesis of silver nanoparticles: Mechanism and biological applications

Abstract

We present the microbial green synthesis of silver nanoparticles (NPs) by Streptomyces ghanaensis VITHM1 strain (MTCC No. 12465). The secondary metabolites in the cell free supernatant of this bacterium when incubated with 1 mmol/L AgNO₃, mediated the biological synthesis of AgNPs. The synthesized AgNPs were characterized by UV-visible spectrum, X-ray diffraction (XRD), atomic force microscope, scanning electron microscopy equipped with energy dispersive spectroscopy, transmission electron microscopy, FT-IR spectroscopy, dynamic light scattering and zeta potential. They were highly stable and, spherical in shape with the average size of 30‒50 nm. The secondary metabolites involved in the formation of AgNPs were identified gas chromatography-mass spectrography. The 3D structure of the unit cell of the synthesized AgNPs was determined using XRD data base. The synthesized AgNPs exhibited significant antibacterial activity against tested bacterial pathogens, and did not show haemolysis on human red blood cells. This green synthesis could provide a new platform to explore and use AgNPs as antibacterial therapeutic agents.

Graphical abstract

Keywords

Streptomyces ghanaensis VITHM1 / nanoparticles / 3D structure / antibacterial activity

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Acknowledgments

We are thankful to the management of VIT University, Vellore, India for providing the facilities to carry out this study.

Conflict of Interests

Both the authors declare that there is no conflict of interests.

Ethical Statement

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Electronic Supplementary Material

Supplementary material is available in the online version of this article at http://dx.doi.org/10.1007/s11705-016-1599-6 and is accessible for authorized users.

Funding

RIGHTS & PERMISSIONS

2016 Higher Education Press and Springer-Verlag Berlin Heidelberg