Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings

Abstract

We have studied the effect of the engineered nanomaterial Taunit, containing multiwalled carbon nanotubes (MWCNTs), on the growth of Onobrychis arenaria seedlings and investigated whether affected plants uptake and accumulate MWCNTs. We found that 100 μg/mL and 1000 μg/mL of Taunit stimulated the growth of roots and stems, and enhanced the peroxidase activity in these parts of plants. Microscopy studies showed the presence of MWCNTs in the root and leaf tissues of seedlings exposed to Taunit, suggesting that MWCNTs have a capacity to penetrate the cell walls, accumulate in roots and translocate to the leaves. Thus the stimulating effect of MWCNTs on seedlings of O. arenaria may be associated with the primary uptake and accumulation of MWCNTs by plant roots followed by translocation to the other plant tissues.

Keywords

multiwalled carbon nanotubes / plants / electron microscopy

References

[1] Handy R D, Owen R, Valsami-Jones E. The ecotoxicology of nanoparticles and nanomaterials: current status, knowledge gaps, challenges, and future needs. Ecotoxicology, 2008, 17(5): 315-325

[2] Moore M N. Do nanoparticles present ecotoxicological risks for the health of the aquatic environment? Environment International, 2006, 32(8): 967-976

[3] Ma X, Geiser-Lee J, Deng Y, Kolmakov A. Interactions between engineered nanoparticles (ENPs) and plants: phytotoxicity, uptake and accumulation. The Science of the total environment, 2010, 408(16): 3053-3061

[4] Navarro E, Baun A, Behra R, Hartmann N B, Filser J, Miao A J, Quigg A, Santschi P H, Sigg L. Environmental behavior and ecotoxicity of engineered nanoparticles to algae, plants, and fungi. Ecotoxicology, 2008, 17(5): 372-386

[5] Ruffini Castiglione M, Cremonini R. Nanoparticles and higher plants. Cariologia, 2009, 62: 161-165

[6] Berhanu D, Dybowska A, Misra S K, Stanley C J, Ruenraroengsak P, Boccaccini A R, Tetley T D, Luoma S N, Plant J A, Valsami-Jones E. Characterisation of carbon nanotubes in the context of toxicity studies. Environmental Health : A Global Access Science Source, 2009, 8(Suppl 1): S3

[7] Yuliang Z, Genmei X, Zhifang C. Are carbon nanotubes safe? Nature Nanotechnology, 2008, 4: 191-192

[8] Poland C A, Duffin R, Kinloch I, Maynard A, Wallace W A, Seaton A, Stone V, Brown S, Macnee W, Donaldson K. Carbon nanotubes introduced into the abdominal cavity of mice show asbestos-like pathogenicity in a pilot study. Nature Nanotechnology, 2008, 3(7): 423-428

[9] Khodakovskaya M, Dervishi E, Mahmood M, Xu Y, Li Z, Watanabe F, Biris A S. Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth. ACS Nano, 2009, 3(10): 3221-3227

[10] Wild E, Jones K C. Novel method for the direct visualization of in vivo nanomaterials and chemical interactions in plants. Environmental Science & Technology, 2009, 43(14): 5290-5294

[11] Lin S, Reppert J, Hu Q, Hudson J S, Reid M L, Ratnikova T A, Rao A M, Luo H, Ke P C. Uptake, translocation, and transmission of carbon nanomaterials in rice plants. Small, 2009, 5(10): 1128-1132

[12] Cañas J E, Long M, Nations S, Vadan R, Dai L, Luo M, Ambikapathi R, Lee E H, Olszyk D. Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species. Environmental Toxicology and Chemistry, 2008, 27(9): 1922-1931

[13] Tkachev A G, Zolotukhin I V. The equipment and technique for synthesis of solid-state nanostructures. Moscow. Mashinostroenie, 2007, 1: 316

[14] Padu E K. Properties of peroxidases and phenylalanine ammonia-lyase in wheat stems during secondary cell wall formation and lignifications. Physiologia Plantarum, 1995, 42: 408-415

[15] Boyarkin A N. The method for fast evaluation of peroxidase activity. Russian Journal of Biochemistry, 1951, 16: 352-355

[16] Pausheva Z P. Plant cell cytology, practical approach. Moscow: Kolos, 1974, 288

[17] Barrena R, Casals E, Colón J, Font X, Sánchez A, Puntes V. Evaluation of the ecotoxicity of model nanoparticles. Chemosphere, 2009, 75(7): 850-857

[18] Chehab E W, Eich E, Braam J. Thigmomorphogenesis: a complex plant response to mechano-stimulation. Journal of Experimental Botany, 2008, 60(1): 43-56

[19] Ostin A, Kowalyczk M, Bhalerao R P, Sandberg G. Metabolism of indole-3-acetic acid in Arabidopsis. Plant Physiology, 1998, 118(1): 285-296

[20] Woodward A W, Bartel B. Auxin: regulation, action, and interaction. Annals of Botany, 2005, 95(5): 707-735

[21] Andreeva V A. Peroxidase and its role in plant defense mechanism. Moscow: Nauka, 1988, 128

[22] Liu Q, Chen B, Wang Q, Shi X, Xiao Z, Lin J, Fang X. Carbon nanotubes as molecular transporters for walled plant cells. Nano Letters, 2009, 9(3): 1007-1010

[23] Serag M F, Kaji N, Gaillard C, Okamoto Y, Terasaka K, Jabasini M, Tokeshi M, Mizukami H, Bianco A, Baba Y. Trafficking and subcellular localization of multiwalled carbon nanotubes in plant cells. ACS Nano, 2011, 5(1): 493-499

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Higher Education Press and Springer-Verlag Berlin Heidelberg