Preparation of hemicellulolic oligosaccharides from Chamaecyparis obtuse (Hinoki) slurry using commercial enzymes

Abstract

Wood biomass is anticipated to serve as a substitute for carbon source, which has no feedstock competition with foods. Biomass is commonly used for the production of bio-ethanol by a series of processes such as pretreatment, enzymatic degradation, and fermentation. Hemicellulose, constituting 20 wt-% – 40 wt-% of biomass materials, contains various kinds of saccharides known to be bioactive substrates. Practical usage of hemicellulose is generally limited to its conversion to bio-ethanol. Here, we aimed to prepare hemicellulolic oligosaccharides, more valuable products other than ethanol. Therefore, the Hinoki slurry was treated with lime at room temperature for 3 h, and then neutralized with HCl. The resulting sample was treated with 13 types of commercial enzymes, and the saccharides produced in the supernatant were evaluated. The result showed that the commercial enzyme Cellulase SS (Nagase & Co., LTD.) effectively degraded the slurry to produce disaccharides and trisaccharides. Analysis of sugar components by liquid chromatography/mass spectrography (LC/MS) after the derivation with ethyl 4-aminobenzoate (ABEE) showed that mannobiose, mannotriose, and cellobiose were the major oligosaccharides. These results indicate valuable oligosaccharides can be successfully produced from Hinoki softwood slurry.

Keywords

hemicellulolic oligosaccharides / Chamaecyparis obtuse (Hinoki) / commercial enzyme / manno-oligosaccharide / cello-oligosaccharide

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Acknowledgments

This work was supported in part by Industry Development Division, Department of Industry and Labor, Okayama Prefectural Government and the Yakumo Foundation for Environmental Science.

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg