Novel polymorphic EST-based microsatellite marker isolation and characterization from Poncirus trifoliata (Rutaceae)

Abstract

Novel Poncirus trifoliata simple sequence repeat (SSR) markers were developed to evaluate their utility for genetic diversity and breeding studies of P. trifoliata and related species. A total of 108 primer pairs were characterized by PCR amplification experiments. Among these, 61 were polymorphic and transferable to other citrus species. The number of alleles per locus ranged from 2 to 6, with an average of 2.37 alleles per locus. The expected heterozygosity and observed heterozygosity ranged from 0 to 0.83 and 0 to 1.00, respectively. These novel polymorphic SSR markers will be useful for citrus cultivar identification and evaluation as well as breeding studies.

Keywords

microsatellite / polymorphism / Poncirus trifoliata

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Acknowledgements

This work was supported by the Ministry of Science and Technology of China (2011CB100601) and the National Natural Science Foundation of China (31330066).ƒ

The online version of this article at http://dx.doi.org/10.15302/J-FASE-2015048 contains supplementary material (Appendix A).

Manosh Kumar Biswas, Peng Chen, Mohamed Hamdy Amar and Xiuxin Deng declare that they have no conflict of interest or financial conflicts to disclose.‚This article does not contain any studies with human or animal subjects performed by any of the authors.

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Higher Education Press and Springer-Verlag Berlin Heidelberg