Very low density lipoprotein (VLDL) was incubated with CuCl₂(10 (μmol/ L) at room temperature for 24 hours. The thiobarbituric acid reactive substance (TBARS) was much higher and the electrophoretic mobility was much faster in VLDL after incubation with CuCl₂ than that in VLDL without incubation with CuCl₂. It demonstrated that VLDL was oxidatively modified by Cu²⁺. Endothelial cells were pretreated with normal VLDL (N-VLDL) and oxidatively modified VLDL (O-VLDL) and then the adhesion of monocyte to endothelial cells was assayed. We observed that O-VLDL at all the concentrations used enhanced monocyte adhesion to endothelial cells significantly. The results suggest that oxidative modification of VLDL may play a role in the early stage of atherogenesis by increasing monocyte adhesion to endothelial cells.

Nested RT-PCR, done by using degenerated primer pair, was used to detect hepatitis C virus RNA (HCV RNA) in serum, plasma, liver and peripheral blood mononuclear cells (PBMC) of 30 patients with acute and chronic posttransfusion hepatitis C and 7 asymptomatic anti-HCV positive subjects. The results showed that the percentages of both the plus and minus strands of HCV RNA in PBMC of the patients with chronic hepatitis C was significantly higher than that with acute hepatitis C and asymptomatic anti-HCV positive subjects (P < 0. 05–0. 001). In 17 patients who were subjected to biopsy, the positive rate of the both strands of HCV RNA in PBMC of the patients with AH was lower than that of CAH (P <0. 05). In serum and plasma of all 37 cases, the minus strand of HCV RNA was not detected. Both plus and minus strands in liver of one patient with AH were positive, but the minus strand in PBMC negative. In 6 patients with CAH whose both strands in liver were positive, both strands in PBMC in 5 patients were also found. The present data confirmed that PBMC of the patients with hepatitis C were infected by HCV and the longer the infection time, the bigger the possibility of PBMC infection by HCV. The patients with active liver disease (CAH) had higher positive rate of minus strands of HCV RNA in PBMC. The results suggested that HCV may not only infect PBMC but also replicate in PBMC, and that the occurrence of minus strand of HCV RNA is associated with activity of liver disease.

By employing the pUC19 as a backbone, the regulatory and signal sequences which encode kanamycin resistance, and mycobacterial plasmid origin of replication (oriM) were cloned into the pUC19. The recombinant E. Coli-mycobacteria shuttle expression plasmid pBCG-8000 was constructed. The pBCG-8000 was able to replicate in both E. Coli and mycobacteria (including BCG) systems, and to confer stable kanamycin resistance upon transformants. The study should facilitate the development of BCG and other mycobacteria into multivalent vaccine vectors.

We used the flow cytometric immunoassay to study the correlation between the tumor-suppressor gene product p53⁻ and the DNA ploidy in 30de novo cases of acute nonlymphocytic leukemia (ANLL). The results showed that 15 cases were negative and the other 15 cases were positive expression for p53. As compared with p53 negative (p53) cases, the patients with positive p53 (p53⁺) had higher percentage of bone marrow blasts and lower peripheral leukocyte and platelet counts, which had no influence on the complete remission rate. Before treatment, DNA diploidy was seen in 18 cases including 12 p53⁻ cases, and DNA aneuploidy in 12 cases including 9 p53⁺. After therapy, aneuploidy could be transformed into diploidy. Patients with P53⁺ or having aneuploidy in complete remission were at risk for early relapse. We believe that p53 may be involved in the process of leukemogenesis and progression of ANLL.

HCV infection among heterosexuals with multiple partners and family members of patients with hepatitis C, and HCV RNA in the body fluid of these patients were investigated. The results showed that the HCV infection in heterosexuals with multiple partners, which was related to sexual activity, was much higher than that of healthy pregnant women. The HCV RNA in the saliva, semen or vaginal discharge of patients with hepatitis C was at detectable level. Among the patient’s relatives, none of the children but 2 spouses were found to be infected with HCV. We concluded that sexual contact might play some role in HCV transmission.

The present study was designed to utilize the chemiluminescence (CL) method for O²⁻ analysis in ischemia and reperfusion of the whole rat liver. The results indicated that the O²⁻ level was lower after 30 and 60 min ischemia. After 40 min reperfusion, the level of O²⁻ increased obviously, reaching about 8. 3-fold (30 min ischemia) and 9. 0-fold (60 min ischemia) (P<0. 001,P<0. 01) respectively. The results also show that Chinese Traditional Medicines ligustrazine and salvia compound can scavenge O²⁻ effectively.

The selection of proper treatment is based on identification of the causes of massive hemorrhage of gastrointestinal tract after pericardial devascularization. The combined use of prostaglandin E₁ indirect portal vein angiography, DSA of celiac artery and color Doppler can provide detailed information about portal vein system, including the presence of embolism, spongioid changes and devasculization of left gastric vein and left gastric artery and the direction of blood flow. If these techniques failed to reveal the causes of digestive tract bleeding, the endoscopy may show lesions of gastric mucosa, which could be accountable for the bleeding.

In order to identify the high risk factors that determine subphrenic infection after hepatectomy, 187 liver resections performed during January, 1985 to December, 1990 in Department of Surgery of Tongji Hospital were reviewed. Thirteen patients developed subphrenic infection with the incidence of subphrenic infection being 6. 95 %. The results of retrospective study on 187 liver resections demonstrated that the high risk factors related to subphrenic infection after hepatectomy were as follows: (1) The extent of liver resection was associated with subphrenic infection. (2) The incidence of subphrenic infection after liver resection of primary liver cancer patients with hepatic cirrhosis was higher than that of non-cirrhotic patients. (3) Intraoperative estimated blood loss greater than 1500 ml was found to be a significant risk factor in the development of postoperative subphrenic infection. (4) Adequate postoperative drainage of subdiaphragm and the raw surface of the liver was one of critical factors of decreasing subphrenic infection after liver resection.

This article, together with literatures, discusses one case of arrhythmia right ventricular dysplasia (ARVD) we treated. This disease should be taken into account in some arrhythmias from right ventricle, especially refractory arrhythmias in youths. Some non-invasive techniques such as echocardiography, cardial single photon emission computed tpmography (SPECT), and magnetic resonance imaging (MRI) play more and more important role in the diagnosis of ARVD. These techniques can provide specific data for the early diagnosis.

Thein vivo release rate of levonorgestrel-containing silicon capsule (in vitro releasing rate: 6 μg/d) placed in rabbits was determined. The results showed that the average release per year was 8. 32 μg withr being 0. 96000. The release pattern of the levonorgestrel-containing silicon capsule system was proved to be zero-order release. Our study provided some experimental data for clinical use of the system for birth control.

To investigate the pathogenesis of retina lesions caused by intraocular pressure elevation, activities and distribution of enzymes in retina including lactic dehydrogenase (LDH), succinate dehydrogenase (SDH), adenosinetriphosphatase (AT-Pase), acid phosphatase (ACP), cholinesterase (ChE), cytochrome oxidase (CCO), nucleotidase (5’-Nase) and glucose-6-phosphatase (G6Pase) were determined histochemically in 30 rabbits. It was found that 1) in the early stage of intraocular pressure elevation, the activities of LDH, SDH, ATPase, ACP, and ChE in retina were increased, while the activities of CCO, 5’-Nase decreased; 2) in the late stage of intraocular pressure elevation, the activities of all these enzymes but ACP, which showed a reduced activity, were close to the normal level; 3) in superoxide dismutase. (SOD-CCE) treated group, except the slight increase of LDH and G6Pase activities, the activities of the remaining enzymes were near to normal. Our results suggest that the various histochemical changes in retina induced by intraocular pressure elevation were compensatory in the early stage and were beneficial to the supply of energy needed in retinal tissue and cellular metabolism; while in the late stage, the lesion of retina cells developed due to decompensation. SOD-CCE could alleviate the retinal lesions caused by intraocular pressure elevation, and can be used as auxiliary drug for the treatment of intraocular pressure elevation.

Coronary artery fistula (CAF) is an abnormal communication between a coronary artery and a cardiac chamber, great vessel, or other vascular structures. The presence of the fistula is usually identified by angiography. In this paper, the diagnosis of left coronary artery-right ventricle fistula was made by color Doppler before angiography? and it was confirmed by surgery. Color Doppler flow imaging is a non-invasive method which can reveal the proximal dilatation, the course and the draining site of CAF.

In this study, we have examined the effects of exposure to high temperature, carbon monoxide or a combination of both conditions in a model system, the rat and in industrial workers. In the rat liver, HSP70 mRNA and HSP70 synthesis were measured by dot hybridization and western blot. The results showed that after a heat stress HSP70 mRNA and its product, HSP70 increased significantly and there was a synergism in the combined effects of high temperature and carbon monoxide exposure on the induction of HSP70 mRNA and HSP70 synthesis. Heat played a major role in this induction. The presence of antibodies to human HSP27, HSP60, HSP70, HSC73, HSP89 α and β in workers exposed to heat, carbon monoxide was also measured by western blot using purified HSPs as antigens. Plasma free amino acids were measured in the same group of workers. The incidence of antibodies to HSP27 and HSP70 was significantly higher in the workers working in an environment with extreme heat, and high carbon monoxide emission than in a control group. The carbon monoxide exposed group showed the highest incidence of antibodies to HSPs. Although our previous results indicated that workers had an insufficient protein intake, plasma free amino acids tended to increase, especially in methionine and tryptophan two kinds of amino acids which are absent from the main stress protein, HSP70. These results suggest that the major problems that these workers may face are how to facilitate the use of plasma free amino acids and reduce the inhibition of synthesis of normal proteins when they are exposed to occupational harmful factors. These results also add new information on the measurement of HSPs as a potential biomonitor to assess whether organisms are experiencing metabolic stress within their environment.

Presented in this paper is a report of the prospective studies on rectal cancer. By using the method of anticancer agent gelatin sponge to form embolism and by ligating the bilateral arteria ilica internal, we performed Miles’ operation on 64 cases of rectal cancer. Only 24 cases in the control group had Miles’ operation. The amount of blood transfused during operation, duration of operation, days of hospitalization, infection rate of incision on the peritoneum, and relapse rate in treated group were significantly different from those in the control group (P<0. 05).

The preliminary experience in the clinical use of Anyang Type 2 esophageal intubation instrument, designed by ourselves, in 11 patients with severe esophageal obstruction due to advanced carcinoma of the esophageal was reported. The diagnosis of all 11 cases was confirmed by barium-meal roentgenography. After intubation, all the patients could take liquid or soft diet by mouth. There was no operative mortality and the patients survived 2 to 14 months (mean 5. 7 months). All of them could take food by mouth before death. The clinical application proved that the intubation has been a safe and suitable management.

Gastric and gallbladder emptying in 113 patients with functional dyspepsia (FD) were evaluated by real-time ultrasonography (RUS) after a liquid-fat meal by the patients, and compared with 15 healthy volunteers. The results showed that in FD group 69 patients (61.06%) had delayed gastric emptying, and 28 patients (24. 77%) had gallbladder hypokinesia. Among them both delayed gastric emptying and gallbladder hypokinesia were found in 11 patients (9. 7%), 44 patients (38. 93%) had normal gastric emptying and 85 patients (75. 22%) had normal gallbladder emptying.