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Frontiers of Optoelectronics

Front. Optoelectron.    2015, Vol. 8 Issue (2) : 170-176     DOI: 10.1007/s12200-015-0480-4
Vascular distribution imaging of dorsal skin window chamber in mouse with spectral domain optical coherence tomography
Jian GAO1,Xiao PENG1,Peng LI2,*(),Zhihua DING2,Junle QU1(),Hanben NIU1
1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
2. State Key Laboratory of Modern Optical Instrumentation, Department of Optical Engineering, Zhejiang University, Hangzhou 310027, China
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Doppler optical coherence tomography or optical Doppler tomography (ODT) has been demonstrated to spatially localize flow velocity mapping as well as to obtain images of microstructure of samples simultaneously. In recent decades, spectral domain Doppler optical coherence tomography (OCT) has been applied to observe three-dimensional (3D) vascular distribution. In this study, we developed a spectral domain optical coherence tomography system (SD-OCT) using super luminescent diode (SLD) as light source. The center wavelength of SLD is 835 nm with a 45-nm bandwidth. Theoretically, the transverse resolution, axial resolution and penetration depth of this SD-OCT system are 6.13 μm, 6.84 μm and 3.62 mm, respectively. By imaging mouse model with dorsal skin window chamber, we obtained a series of real-time OCT images and reconstructed 3D images of the specific area inside the dorsal skin window chamber by Amira. As a result, we can obtain the clear and complex distribution images of blood vessels of mouse model.

Keywords optical coherence tomography (OCT)      mouse      dorsal skin window chamber      vascular distribution     
Corresponding Authors: Peng LI   
Just Accepted Date: 11 March 2015   Online First Date: 01 April 2015    Issue Date: 24 June 2015
 Cite this article:   
Peng LI,Zhihua DING,Junle QU, et al. Vascular distribution imaging of dorsal skin window chamber in mouse with spectral domain optical coherence tomography[J]. Front. Optoelectron., 2015, 8(2): 170-176.
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Peng LI
Zhihua DING
Junle QU
Hanben NIU
Jian GAO
Fig.1  Schematic diagram of spectral-domain OCT system. CMOS: complementary metal oxide semiconductor; PC: personal computer
Fig.2  Output spectrum curve of the optical source
Fig.3  Dorsal skin window chamber model
Fig.4  Vascular distribution images of dorsal skin window chamber mouse model. (a) Real-time OCT image; (b) 3D image of the vascular distribution; (c) and (d) blood vessel distributions at two different depths, which are 80 and 320 μm, respectively. Scale bar in (a) is 300 μm in longitudinal direction, while the scale bar in transverse direction is 100 μm. Scale bars in (b), (c) and (d) are 100 μm
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