Antibacterial and biological properties of silver-loaded coralline hydroxyapatite

Abstract

The antibacterial and biological properties of silver-loated coralline hydroxyapatite (Ag-CHA) as a new antibacterial implant material were investigated in this study. Compared to other antibiotic and chemical bactericidal agents, Ag⁺ does not bring bacterial resistance to drugs and has less toxicity. The porous CHA was formed by hydrothermal exchange, then Ag⁺ was loated onto CHA through ion exchange and adsorption. The microstructure and composition of Ag-CHA were characterized by scanning electron microscopy (SEM), Rutherford backscattering spectrometry (RBS), and energy dispersive spectrometry (EDS). Antibacterial activity of Ag-CHA on the clinical strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) was evaluated by the flat plate diffusion method. The antibacterial activity of Ag-CHA was found to be correlated with the concentration of Ag⁺ in a dose-dependent manner, which indicated that the optimal antibacterial and biocompatible effects of Ag-CHA could be obtained with Ag⁺ concentrations from 5×10^-5 to 1×10^-4 mol/L.

Keywords

silver-loated loralline hydroxyapatite (Ag-CHA) / antibacterial activity / cytotoxicity

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Acknowledgements

We are grateful for the support of the Medical and Health Research Fund of PLA (Grant No. 2006163045), the National Innovation Fund of Medium or Small Science and Technology Enterprise (Grant No. 206404), Guangzhou Science and Technology Key Project (Grant No. 206402) and Guangzhou Huadu County Science and Technology Project (Grant No. HD06G006).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg