iTRAQ-based quantitative analysis of cancer-derived secretory proteome reveals TPM2 as a potential diagnostic biomarker of colorectal cancer

Abstract

Colorectal cancer (CRC) is a leading cause of cancer-related deaths worldwide. We aimed to find novel molecules as potential biomarkers for the early diagnosis of CRC. A serum-free conditioned medium was successfully collected from three pairs of CRC tissue and adjacent normal tissue. iTRAQ-based quantitative proteomic analysis was applied to compare the differences in secretome between primary CRC mucosa and adjacent normal mucosa. A total of 145 kinds of proteins were identified. Of these proteins, 29 were significantly different between CRC and normal tissue. Tropomyosin 2 β (TPM2) exhibited the most significant differences; as such, this protein was selected for further validation. Quantitative real-time PCR indicated that the mRNA expression of TPM2 significantly decreased in the CRC tissue compared with the paired adjacent normal tissue. Immunohistochemical analysis also confirmed that TPM2 was barely detected at protein levels in the CRC tissue. In summary, this study revealed potential molecules for future biomarker applications and provided an efficient approach for the differential analysis of cancer-associated secretome. TPM2 may be valuable for the early diagnosis of CRC.

Keywords

iTRAQ / secretome / colorectal cancer / TPM2

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Acknowledgements

This work was financially supported by National Basic Research Program of China (973 Program, No. 2011CB910700) and National Natural Science Foundation of China (No. 81201966).

Compliance with ethics guidelines

Yiming Ma, Ting Xiao, Quan Xu, Xinxin Shao, and Hongying Wang declare that they have no conflict of interest. All procedures related to human samples were approved by the Review Board of Chinese Academy of Medical Sciences Cancer Institute. Informed consent was obtained from all patients for inclusion in the study.

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2016 Higher Education Press and Springer-Verlag Berlin Heidelberg