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Protein aggregation in association with delayed
neuronal death in rat model of brain ischemia
- GE Pengfei1, FU Shuanglin1, LI Wenchen1, WANG Chonghao1, ZHOU Chuibing1, LUO Yinan1, LUO Tianfei2
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1.Department of Neurosurgery, First Hospital of Jilin University; 2.Norman Bethune Medicine School, Jilin University
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History
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| Published |
| 05 Mar 2008 |
| Issue Date |
| 05 Mar 2008 |
To investigate the relationship between protein aggregation and delayed neuronal death, we adopted rat models of 20 min ischemia. Brain ischemia was produced using the 2-vessel occlusion (2VO) model in rats Light microscopy, transmission electronic microscopy and Western blot analysis were performed for morphological analysis of neurons, and protein detection. The results showed delayed neuronal death took place at 72 h after ischemia-reperfusion, protein aggregates formed at 4 h after reperfusion and reached the peak at 24 h after reperfusion, and Western blot analysis was consistent with transmission electronic microscopy. We conclude that protein aggregation is one of the important factors leading to delayed neuronal death.
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References
1. Kirino T Delayedneuronal death in the gerbil hippocampus following ischemiaBrain Res 1982 239(1)5769
2. Martone M E Jones Y Z Young S J Ellisman M H Zivin J A Hu B R Modificationof postsynaptic densities after transient cerebral ischemia: a quantitativeand three dimensional ultrastructural studyNeuroscience 1999 19(6)19881997
3. Hu B R Janelidze S Ginsberg M D Busto R Perez-Pinzon M Sick T J Siesjö B K Liu C L Protein aggregation after focalbrain ischemia and reperfusionJ Cereb BloodFlow Metab 2001 21(7)865875
4. Grune T Jung T Merker K Davies K J Decreased proteolysiscaused by protein aggregates, inclusion bodies, plaques, lipofuscin,ceroid and aggresomes during oxidative stress, aging and diseaseInt J Biochem Cell Biol 2004 36(12)25192530
5. Ardley C H Hung C C Robinson A P The aggravating role of the ubiquitin proteasome systemin neurodegenerationFEBS Letters 2005 579(3)571576
6. Hegde N A Ubiquitin-proteasomemediated local protein degradation and synaptic plasticityProg Neurobiol 2004 73(5)311357
7. Kopito R R Aggresomes,inclusion bodies and protein aggregationTrends Cell Biol 2000 10(12)524530
8. Stefani M Dobson C M Protein aggregation and aggregatetoxicity: new insights into protein folding, misfolding diseases andbiological evolutionJ Mol Med 2003 81(11)678699
9. Squier C T Oxidativestress and protein aggregation during biological agingExp Gerontol 2001 36(9)15361550
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