On the basis of preparation of anti-metastatic recombinant FN polypeptides, CH50 and CH56, we further studied the function of these polypeptides. The capacity of CH50 binding with melanoma cells (ED50 30 mM) was higher than that of CH56 (ED50 45 mM). Both of the polypeptides could significantly suppress the binding of melanoma B16 cells to laminin. There was no significant difference in the inhibitory effect between two polypeptides. In the experimental metastasis of melanoma cells, both of CH50 and CH56 could significantly inhibit the metastasis of the tumor cells, and reduce the number of lung metastasis by about 80 %. Our results suggest that III-11 and ED-A repeats influenced, to some extent, the binding capacity of bifunctional-domain polypeptide to cells, but did not affect the inhibition of the polypeptide on the metastasis of melanoma cells. The presence and connection of cell I and Hep II domains are the elements which determine the ability of recombinant FN polypeptides to inhibit the metastasis of tumor cells.
Growth hormone releasing peptide (GHRP-6) is a synthetic hexapeptide which specifically stimulates secretion of growth hormone (GH) by pituitary somatotrophs. Phorbel ester, 1,2 tetradecanoylphorbol 13 acetate (TPA) can also stimulate releasing of GH. The precise intracellular mechanism has not been entirely deciphered. We used cell cultures of human pituitary somatotrophinomas to investigate the relation between GHRP-6 and TPA on membrane phosphatidylinositol (PI) turnover and GH secretion. The results showed that the working mechanisms of GHRP-6 and TPA are not identical, although they all can stimulate GH secretion in human pituitary somatotrophinomas. This indicates that PI-PKC signal transduction system may play a crucial role in the regulation of GH secretion.
The purpose of this study were to confirm whether the modified treatment with L-glutamic acid could attenuate the calcification of the GA-fixed valves and improve its biocompatibility. Pericardial valves were routinely treated with GA and valves were treated with GA and 8 % L-glutamic acid. The valves treated with these methods were implanted subcutaneously in rats. Calcium deposits of the valves collected at the 7th, 21st, 60th, 90th day were assessed by atomic absorption spectroscopy, and the pathologic changes were examined by light and electron microscopy. Cultured endothelial cells (ECs) were seeded onto the valves. The cell counts were determined at the 1st, 4th, 7th, 10th day after seeding. PGI2 in culture medium was tested at the 10th day. Transmission and scanning electron microscopy were used to observe the growth of ECs on the valves. Results showed that subsequent treatment with L-glutamic acid could significantly mitigate calcification of bovine pericardial valves as compared with simple GA-fixed valves (P<0. 01). ECs seeded on the GA treated valves died within 4 days. On the valves treated by modified method, ECs could proliferate and release PGI2. It is concluded that treatment with L-glutamic acid can markedly inhibit the calcification and improve the biocompatibility of bioprosthetical valves.
The present study was undertaken to figure out the source of urinary endothelin (ET) and the clinical significance of its possible variations. Urinary ET levels were measured by radioimmunoassay (RIA) in 17 healthy newborns and 20 asphyxiated neonates on days 1,3,7 after birth. Plasma ET concentrations of healthy premature infants on day 7 and urinary ET levels in 10 healthy children were also observed at the same time. Results showed that: (1) Urinary ET levels and ET excretion rates were higher than plasma ET in preterm infants on days 7 after birth; (2) Both in preterm and full term infants, urinary ET concentrations fell from the 1st day to the 7th day after birth, ET excretion rates elevated markedly at the end of the 1st week, and they were significantly higher than that of children; (3) Urinary ET levels of asphyxiated group on days 1 and 3 were much higher than those of healthy neonates, and positively correlated with the severity of the illness and urinary NAG. We conclude that: (1) urinary ET mainly comes from the production in renal cells; (2) ET levels in healthy neonatal urine reflect the maturity of kidney; (3) measurement of urinary ET levels in asphyxiated neonates is helpful to judge the degree and to evaluate the prognosis of renal injury.
Urinary epidermal growth factor (EGF) excretion in normal newborn as well as neonates with asphyxia was investigated by using radioimmunoassay, and serum creatinine (Scr) levels determined at the same time. The results showed that in severe asphyxia group the ratio of urinary EGF to urinary creatinine (Cr) (EGF/Cr), an index reflecting EGF excretion, was decreased on the first day (P<0. 05) and reached the lowest level on the third day (P<0. 01). However, EGF/Cr values were decreased only on the third day in neonates with mild asphyxia (P<0. 05). On the seventh day, EGF/Cr values of neonates with asphyxia rose to normal. There were a negative correlation between urinary EGF/ Cr and Scr. It is suggested that EGF may play a role in the repair of acute renal injury after asphyxia and the detection of urinary EGF concentration is useful in the judgment of severity of renal injury and in the evaluation of the recovery of renal tubule after injury.
To investigate the reversing effects of DDPH on cardiac hypertrophy and increased collagen content in left ventricle tissue of rats, cardiac hypertrophy of rats were induced by partial narrowing of abdominal aorta. 4 weeks after operation, the rats were given DDPH for 8 weeks. 12 weeks later, it was found that in model group, LVW/WHW and WHW/BW increased by 39. 0 % and 36. 9 % than those in control group; collagen content increased by 1. 5 times. IΦ/EΦ, LS decreased (P<0. 01), MMW/EΦ, WZ increased (P<0. 01). The above-mentioned changes in two DDPH groups could be partly or completely reversed. It is concluded that DDPH could reverse cardiac hypertrophy of rats induced by partial narrowing of abdominal aorta and reduce collagen content in left ventricle tissue.
By using receptor autoradiography to observe the distribution and density of receptors, the effects of propranolol, a β-blocker, on β-adrenergic receptor of experimental acute myocardial infarction (AMI) were studied. One week after ligation of proximate left anterior descend (LAD) coronary artery, [3H] DHA binding sites were markedly decreased in both infarctregion and non-infarct region. After treatment of propranolol (100 μg/kg), the [3H] DHA binding sites were obviously increased in the infarct region, and they were further decreased in the non-infarct region. The ratio of [3H] DHA binding sites of the infarct region to non-infarct region was from 0. 24 at LAD ligation to 0. 87 after propranolol treatment, which was close to 0. 97 of control group (sham operation). The results indicated that the propranolol acted directly on myocardial β- adrenergic through the receptor regulation of the balance of β-receptors between the infarct region and non-infarct region, and improvement of the myocardial consonation and contraction synergism, thereby protecting the heart affected by AMI.
The aim of this study was to explore appropriate index for load evaluation by analyzing the changes of pulmonary ventilation (VE) and heart rate (HR) in 35 student volunteers under the condition of static posture and weight holding. Each subject was asked to perform 7 kinds of static postures and 2 kinds of weight holdings. The HR was continuously measured by a remote detecting device. VE was recorded by a ventimeter for an average of 5 min. The results showed that HR was positively correlated to VE under static activities (r=0. 89), which suggested that VE as well as HR could to some degrees reflect the static load. It was better to use VE to indicate the load of weight holdings than to use HR, which, on the other hand, was more sensible in reflection of the posture load. It is better to use relative value of both of VE and HR to reflect the static load. In addition, arm-overheaded posture increase HR more significantly than any other activities.
A new drug delivery system (DDS), ciprofloxacine/tricalcium phosphate delivery capsule (CTDC) was developed by loading a broad-spectrum antibiotic ciprofloxacine into the central cylindrical cavity of tricalcium phosphate capsule. The new system showed good biocompatibility and could degrade gradually in our preliminary studies.In vitro study showed that CTDC could mantain high-level and long-term releae of ciprofloxacine and that ultrasonic irradiation within the range of physical therapy could increase the drug release amount from CTDC (P<0. 02), and might become a new technique to achieve the control of drug release from DDS.
The effect of anti-hypertensive drug amlodipine on regression of cardiovascular hypertrophy due to hypertension was studied by using cultured smooth muscle cells derived from arteries of spontaneously hypertensive rats (SHR) and measuring [3H]-TdR and [3H]-Leucine binding. 48 h after adding amlodipine, [3H] -TdR binding in arterial smooth muscle cells from SHRin vitro was reduced by 50. 5 % and [3H]-Leucine binding was reduced by 56. 2 % as compared with neuropeptide Y(NPY)-treated group. However, there was no significant change in cell number. The results showed that amlodipine could effectively inhibit increase of DNA and protein synthesis of vascular smooth muscle cell (VSMC) due to NPY. It indicates that amlodipine is of great significance on regression of genesis and development of cardiovascular hypertrophy due to hypertension.
Presented in this paper are the results of ultrasounic examination in 29 cases of hemangiomas of soft tissue. Out of the 29 cases, 20 lesions were revealed as heterogeneous echoic masses; 6 as anechoic areas and 3 as solid masses. Hyperechoic foci of calcifications or phlebolithes were detected in 16 cases. Intramuscular hemangiomas were sonographically diagnosed in 15 patients; hemangiomas of soft tissue in 8, pseudoaneurysm in 3; deformity of the arteriovenous fistula in 1 and hemangiosarcoma in 2. The diagnosis in 28 patients were surgically and pathologically confirmed and the remaining one was confirmed by aspiration and cytological examination. The diagnostic accuracy of the ultrasound was 100 %.
A clinical study was conducted to compare the analgestic effect of clonidine with those of sumatriptan and their mixture and their effects on hemodynamics. 40 patients undergoing elective total hysterectomy were randomly divided into 4 groups in terms of the epidurally administered drugs with 10 patients in each group (group C1: clonidine 150 μg, group C2: clonidine 75 μg, group S: sumatriptan 6 mg and group S+C:clonidine 75 μg + sumatriptan 3 mg). MAP, HR, SpO2, VT VAS, VRS and ePDT were measured in the initial 4 h. The demographic data and the doses of intraoperative local anesthetics among the 4 groups were not staistically different. It was found that no significant difference in the pre- and postadministration values of HR, SpO2 and VT. A obvious reduction of MAP appeared in the groups of C1 and C2, but in the other groups the hemodynamical parameters were stable. The groups of C1, C2 and S+C showed significant increase in VAS and VRS, along with increase of ePDT when comapred with the pre-drug level. There was no obvious alteration in group S after the drug administration. It was concluded that (1) single use of sumatriptan is ineffective in the dose given in this study; (2) small dose (150 μg and 75 μg) of clonidine has epidurally analgesic effects; (3) combined use of sumatriptan and clonidine is an acceptable way in epidural analgesia, in terms of its analgesic effect and hemodynamic changes.
The purpose of this study was to determine the prevalence, clinical significance, and embolic potential of thoracic aortic plaque in patients with cerebral ischemia and to further study the correlation of aortic plaque with carotid or heart disease. We used transesophageal echography (TEE) to evaluate potential source of emboli in aortic arch and heart, and duplex in carotid artery. A atherosclerotic lesion of aortic arch was defined as normal (0), mild plaque (1), moderate plaque (2) and protruding plaque or mobile plaque (3). 75 of 100 patients were found to have atherosclerotic lesion in aortic arch. 16 of 75 patients over degree 2 exhibited no pathologic finding of heart or carotid and 4 of 16 patients were classified as degree 3. The pathologic findings of heart and carotid were significantly correlated with aortic plaque. Age, diabetes, CAD were also significantly correlated with aortic plaque. Aortic atherosclerosis was common in cerebral ischemia. Aortic plaque might be responsible for not only some unexplained embolic events, but also for some of the embolie stroke in patient who have carotid artery or heart disease. Age, diabetes, CAD might be important risk factors in the development of atherosclerotic lesion in the aortic arch.
The condition of acute cholangitis of severe type (ACST) develops very rapidly and the prognosis is poor. The main clinical feature is that there is an obvious dynamic variation, which is the principal factor for the early occurrence of shock and death. In this study the Japanese long ear rabbits were used and biliary tract pressure increasing and splanchnic nervous plexus blocking experiments were conducted. Our results indicate that 0. 6 % of lidocaine can be used to block the right celiac plexus. It can avoid the decrease of blood pressure due to the pressure increase of the biliary tract, and the decrease of blood pressure due to the pressure increase of the biliary tract can be corrected by local anesthesia. 8 cases in conformity with the ACST diagnostic standards received the right renal capsule block injection for the purpose of stopping the celiac plexus, 6 cases of whom had a return of blood pressure to various extent, suggesting that the splanchnic nervous activity in the occurrence of ACST is of great significance. The technique may provide a new approach for the clinical treatment of ACST.
From Jan. 1995 to June 1996, 6 cases of traumatic defect of the tibia were treated by remodeling and reduction of fractured ends and osteotomy and distraction at the proximal metaphysics of the tibia. In 6 patients, aged 19 to 54, with the limb-length discrepancy being at least 3 to 4. 5 cm, the tibia was lengthened by 3 cm in 3 cases, 4 to 4. 5 cm in 3 cases. The consolidation and maturation of the bone were accomplished within 3 to 6 months. The authors believe that this procedure is simple, less invasive and involves no bone-graft.
The paper introduces a new type instrument for rapid measuring ATP. The system consists of a micromodule ATP sensor and an instrument for measuring weak light transmitted by optic fiber. The micromodule ATP sensor mainly is composed of enzyme membrane, a probe and a bundle of optic fiber. The instrument measuring weak light consists of photomultiplier, high voltage power, pulse amplifier and counter. The instrument was characterized by simple structure, small size, rapid response time (<5 s), high sensitivity (10−12 mol/L), stable performance (measuring the same sample for 50 times, CV < 5 %), long enzyme storage time (>3 months).