The effect of high cholesterol, high fat diet on rabbit plasma β-VLDL and its interaction with macrophage

Abstract

Rabbits were fed on 1 % cholesterol and 10 % rape seed oil (group A) or lard (group B). Plasma cholesterol and triglyceride level of group A was strikingly lower than that of group B. No difference was found in the lipid composition of β-VLDLs between both groups. However, apo E content of β-VLDL from group A was higher and apo B (higher molecular weight) was lower than that of group B respectively. β-VLDL of group A caused greater accumulation of cholesterol in mouse peritoneal macrophage than that of group B. Therefore, we assume that the good effect of poly-unsalurated fatty acid on atherosclerosis results not from decreasing accumulation of cholesterol in macrophages but from enhancing elimination of β-VLDL by the liver.

Keywords

poly-unsaturated fatty acid / β-VLDL / maciophage / atherosclerosis / foam cell

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