On the induction of sister chromatid exchange by diethylstilbestrol and Mitomycin C in lymphocytes of mothers and their newborn infants

Abstract

Lymphocytes of mothers and their newborn infants were treated with varying concentrations of diethylstilbestrol (10⁻⁶M, 10⁻⁵ M and 10⁻⁴M) and mitomycin C (0.003, 0.009 and 0.03 μg/ml), and the incidence of sister chromatid exchange (SCE) was determined. The results showed that the spontaneous SCE rate in the newborn is less than that of their mothers. A dose-related increase in SCEs was oberserved in cells exposed to the alkylating chemical mitomycin C, while increases in SCEs were not noted in cultures exposed to diethylstilbestrol. No different susceptibilities of lymphocytes from mothers and newborns to the chemical induction were observed.

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