Electron microscopic observation of interaction between normal mononuclear cells and platelets from ITP children

Abstract

We used radioimmunoassay to detect platelet-associated IgG and also observed with the electron microscope the interaction between normal mononuclear cells and platelets from ITP children as well as the effect of SPA treatment on the interaction. It was confirmed that ITP was related to the increase of platelet-associated IgG and that platelet-associated IgG did exist on ITP platelets. It was also made clear preliminarily that platelet-associated IgG was bound to ITP platelets mainly through its F_{a b} terminus with its F_c fragment exposed freely.

Keywords

platelet / mononuclear cells / idiopathic thrombocytopenic purpura (ITP) / staphylococcal protein A (SPA)

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