Effect of monocyte chemotactic protein-1 on the intraperitoneal adhesion formation

Abstract

In order to study the role of monocyte chemotactic protein-1 (MCP-1) in the infra-peritoneal adhesion formation, 23 infertile patients undergoing laparoscopic operation were divided into two groups: experimental group including 12 patients with intra-peritoneal adhesion and control group including 11 patients without intra-peritoneal adhesion. Peritoneal fluid (PF) and peritoneum were collected from these patients during laparoscopic examination. The expression levels of MCP-1 protein and MCP-1 mRNA were detected by using enzyme-linked immunosorbent assay (ELISA) and dot blot analysis method respectively. It was found that the levels of MCP-1 protein in PF of the patients with peritoneal adhesion were significantly higher than in the control group (0. 44 ± 0. 11 ng/ ml vs 0. 19 ± 0. 09 ng/ml respectively,P < 0. 01). The level of MCP-1 mRNA in the peritoneum of the patients with peritoneal adhesion was significantly higher than in the control group (48. 61 ± 3. 72 vs 19. 87 ± 2. 54 respectively,P < 0. 01). It was suggested that MCP-1 might play a role in the adhesion formation, and chemotactic cytokines expressing in the peritoneal mesothelial cells might be take part in the process.

Keywords

peritoneal adhesion / monocyte chemotactic protein-1 / peritoneal fluid / peritoneum

References

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