Study on biologic activity for membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus

Abstract

Using DPH fluorescence probe, the membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus (EHFV) was labeled. The membrane lipid fluidity was obviously decreased from the membrane lipid fluorescence polarization. The membrane lipid fluidity of lymphocyte, monocyte and neutrophilic granulocyte was dynamically observed. After culturing the cells for 1, 6, 24 and 72 h, it was found that all the membrane lipid fluidity of the infected cells was decreased obviously with the longer the culturing time, the more obvious it. Compared with the normal control groups, there was a significant difference statistically (P < 0. 05–0. 01). It was suggested that the decrease of the membrane lipid fluidity of normal bone marrow cell with infection of EHFV had correlation with the degree of virus invading and cellfunction injury.

Keywords

epidemic hemorrhagic fever virus / bone marrow cell membrane lipid fluidity

References

[1] 1989,4:250

[2] ShinitzkyM, BarenholzY. Fluidity parameters of lipid regions determined by fluorescence polarization. Biochimbiophys Acta, 1978, 515: 367-367

[3] YanovichS. Dynamic parameters of membrane lipids in normal and leukemia human lymphocytes isolated from peripheral blood and bone marrow. Cancer Res, 1978, 38: 4654-4654

[4] 1988, 1:41

[5] 1992,6:435