Effects of L-THP on Ca²⁺ overload of cultured rat cardiomyocytes during hypoxia and reoxygenation

Abstract

The effects of L-tetrahydropalmatine (L-THP) on the cultured rat cardiomyocytes during hypoxia and reoxygenation and the mechanism of L-THP treating reperfusion-arrythmias were studied. The concentration of intracellular free calcium ([Ca²⁺]_i) of single cultured ventricular myocyte was determined by using EPC-9 light-electricity measurement system. It was found that L-THP (100 μmol/L) could reduce the [Ca²⁺]_i augmentation in single cultured ventricular myocyte during hypoxia and reoxygenation. Verapamil (10 μmol/L) had the similar effect. It was concluded that LTHP could inhibit the Ca²⁺ overload of cultured rat cardiomyocytes during hypoxia and reoxygenation.

Keywords

hypoxia-reoxygenation injury / free calcium / cardiomyocyte culture / L-tetrahydropalmatine

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