Research on normal human bone marrow CFU-GM by limiting dilution microculture

Abstract

By using the newly developed technique of limiting dilution microculture we have succeeded in cultivating CFU-GM of human bone marrow. The clones, developed by this method, could be clearly and well identified, and the cells could be easily separated as compared with those developed by semisolid agar culture. By using linear regression analysis,³H-Td R and scanning electron microscopy, this method has been further proved to be superior in accuracy to other procedures.

Keywords

limiting dilution microculture / human CFU-GM / scanning electron microscopy

References

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