Enhancement of B cell responses by the interaction of CD2 with LFA-3

Abstract

Potential effect of the interaction of CD₂ with its ligand LFA-3 on B cell responses in vitro was studied to evaluate the action of CD₂-LFA-3 in immune responses. It was found that: 1) addition of autologous erythrocytes to uniractional mononuclear cells resulted in enhancement of PWM-induced lymphocyte proliferation, and had no significant influence on non-mitogen stimulation; 2) autologous erythrocytes potentiated the synthesis of immunoglo bulins; and 3) costimulating effects of autologous RBC can be depressed by preincubation of PBMNC with anti-CD₂ monoclonal antibody (WuTll). The results suggest that preincubation of mononuclear cells with anti-CD₂ McAb has a down-regulating effects on B cell responses and the interaction of CD₂ with its ligand LFA-3 expressed by RBC is closely related to immune responses.

Keywords

CD₂ / lymphocyte function associated antigen 3 (LFA-3) / lymphocyte proliferation test / antibody synthesis / anti-CD₂ McAb

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