The effect of interleukin-6 on the proliferation of prostate cancer cellsin vitro and the modulation of this procedure

Abstract

The role of interleukin-6 (IL-6) in the growth of an androgen-independent prostate cancer cell line (PC-3m) was defined and the effect of dexamethasone, which was previously shown to modulate IL-6/IL-6 receptor (IL-6R) on this procedure was investigated. By using a pretty sensitive and specific enzyme immunoassay (ELISA), it was found that PC-3m produced certain IL-6, but there was no difference in IL-6 secretion between the group with or without dexamethasone treatment. It was also found that PC-3m cells could not be stimulated to grow by exogenous IL-6 (P>0. 05), while it could be inhibited to grow by anti-IL-6 monoclonal antibody and dexamethasone with a dose-dependent fashion. Our observation indicated that IL-6 acted as an autocrine growth factor for PC-3m, and dexamethasone could inhibit cell proliferation by a mechanism independent of its effect on IL-6 mRNA expression.

Keywords

interleukin-6 / prostate cancer / dexamethasone / crystal violet

References

[1] KellerE T, WanagatJ, ErchlerW B. Molecular and cellular biology of interleukin-6 and its receptor. Front in Bios, 1996, 12340-340

[2] MasatoO, ChungL, RyoichiO. Interleukin-6 as a paracrine and autocrine growth factor in human prostatic carcinoma cellsin vitro. Cancer Res, 1997, 57141-141

[3] DegeorgesA, TatoudR, Fauvel-LafeveF, et al. . Stromal cells from human benign prostate hyperplasia produce a growth-inhibitory factor for LNCaP prostate cancer cells, identified as interleukin-6. Int J Cancer, 1996, 68207-207

[4] SiegsmundM J, YamazakiH, PastanI. Interleukin-6 receptor mRNA in prostate carcinomas and benign prostate hyperplasia. J Urol, 1994, 1511396-1396

[5] BorsellinoB, BelldegrunA, BonavidaB. Endogenous interleukin6 is a resistance factor for cis-Diamminedichloroplatinum and etoposide-mediated cytotoxity of human prostate carcinoma cell lines. Cancer Res, 1995, 554633-4633