Anti-viral activity of hairpin ribozyme directed against HBV core regionin vitro

Abstract

To study the preparation and cleavage of hairpin ribozyme (HpRz) directed against the transcript of HBV core regionin vitro, HRz gene designed by computer targeting the transcript of HBV core gene was cloned into the vector p1. 5 between 5′-cis-Rz and 3′-cis-Rz.³²P-labeled HpRz transcript proved whether the vector fit for the preparation of hairpin ribozyme.³²P-labeled pKC transcript containing HBV core region as targets-RNA was transcribed by using T₇ RNA polymerase and purified by PAGE. Cold HpRz transcript was incubated with³²P-labeled target-RNAs under different conditions and radioautographed after denaturing polyacrylamide gel electrophoresis. The results showed that HpRz had the ability of cleavage at 37°C and 12 mmol/L MgCl₂ and the design of ribozyme was correct. It is concluded that HpRz preparedin vitro possesses specific catalytic activity, indicating that it is possible for HpRz to intracellularly inhibit the replication of HBV. It may be developed into a nucleic acid drug in the treatment of hepatitis B in the future.

Keywords

hairpin ribozyme / hepatitis B virus / plasmid-independent transcription / RNA cleavage

References

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